Auchere, F., R. Sikkink, C. Cordas, P. Raleiras, P. Tavares, I. Moura, and J. Moura. "
{Overexpression and purification of Treponema pallidum rubredoxin; kinetic evidence for a superoxide-mediated electron transfer with the superoxide reductase neelaredoxin}."
J Biol Inorg Chem. 9 (2004): 839-849.
Abstract{Superoxide reductases are a class of non-haem iron enzymes which catalyse the monovalent reduction of the superoxide anion O2- into hydrogen peroxide and water. Treponema pallidum (Tp), the syphilis spirochete, expresses the gene for a superoxide reductase called neelaredoxin, having the iron protein rubredoxin as the putative electron donor necessary to complete the catalytic cycle. In this work, we present the first cloning, overexpression in Escherichia coli and purification of the Tp rubredoxin. Spectroscopic characterization of this 6 kDa protein allowed us to calculate the molar absorption coefficient of the 490 nm feature of ferric iron
Zhang, S., L. Raniero, E. Fortunato, L. Pereira, N. Martins, P. Canhola, I. Ferreira, N. Nedev, H. Águas, and R. Martins. "
{Characterization of silicon carbide thin films prepared by VHF-PECVD technology}."
Journal of Non-Crystalline Solids. 338-340 (2004): 530-533.
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G. Blair, L. Blair, A. Rashid, A. Moreira, J. Araújo, and R. Chitchyan. "
Engineering Aspect-Oriented Systems."
Aspect-Oriented Software Development . Robert E. Filman, Tzilla Elrad, Siobhan Clarke, Mehmet Aksit ed. Addison-Wesley, 2004.
Abstract