This work reports for the first time a resonance Raman study of the mixed-valence and fully reduced forms of Paracoccus pantotrophus bacterial cytochrome c peroxidase. The spectra of the active mixed-valence enzyme show changes in the structure of the ferric peroxidatic heme compared to the fully oxidized enzyme; these differences are observed upon reduction of the electron-transferring heme and upon full occupancy of the calcium site. For the mixed-valence form in the absence of Ca2+, the peroxidatic heme is six-coordinate and low-spin on the basis of the frequencies of the structure-sensitive Raman lines: the enzyme is inactive. With added Ca2+, the peroxidatic heme is five-coordinate high-spin and active. The calcium-dependent spectral differences indicate little change in the conformation of the ferrous electron-transferring heme, but substantial changes in the conformation of the ferric peroxidatic heme. Structural changes associated with Ca2+ binding are indicated by spectral differences in the structure-sensitive marker lines, the out-of-plane low-frequency macrocyclic modes, and the vibrations associated with the heme substituents of that heme. The Ca2+-dependent appearance of a strong gamma(15) saddling-symmetry mode for the mixed-valence form is consistent with a strong saddling deformation in the active peroxidatic heme, a feature seen in the Raman spectra of other peroxidases. For the fully reduced form in the presence of Ca2+, the resonance Raman spectra show that the peroxidatic heme remains high-spin.

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This seminar was a presentation of S.Valtchev on the methods of wireless energy transfer. The seminar was invited by the colleagues of the Power Processing Laboratory of TU Delft, the Netherlands.

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Software transactional memory (STM) is a promising programming model that adapts many concepts borrowed from the databases world to control concurrent accesses to memory (RAM) locations. In this paper we propose a new classification for the active states of a transaction; a new memory quiescing algorithm, to allow the safe transition of a memory block form transactional to non-transactional space; we compare word and object transactional grain units; and evaluate the cost of consistent state validation, arguing that this cost can be minimized by performing partial validation on problematic code regions.

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The richness of Late Jurassic vertebrates in Portugal is known since the 19th century by Paul Choffat, Henri Sauvage and other. The Kimmeridgian Guimarota fauna assemblage is the best known, followed by the fauna of Lourinhã formation. Here is presented an attempt to provide a checklist of the reptiles and amphibians of the Late Jurassic. Amphibia: Lissamphibia (Celtedens, cf. Marmorerpeton, Discoglossidae indet.). Chelonia: Eucryptodira (Pleurosternidae indet., Platychelyidae indet., Plesiochelys cf. etalloni, Plesiochelys choffati, Anosteirinae indet.). Squamata: Scincomorpha (Becklesius hoffstetteri; Paramacellodus sp., Saurillodon proraformis, S. henkeli, S. cf. obtusus). Squamata: Anguimorpha (Dorsetisaurus pollicidens, Parviraptor estesi). Crown Lepidosauromorpha (Marmoretta sp.). Choristodera: Cteniogenidae (Ctenogenys reedi). Sauropterygia: Plesiosauria: Cryptoclidoidea: Cryptoclididae indet. Crocodylomorpha (Lisboasaurus estesi, L. mitrocostatus). Crocodyliformes: Neosuchia (Machimosaurus hugii, Goniopholis cf. simus, Goniopholis baryglyphaeus, cf. Bernissartia, Atoposauridae, Theriosuchus guimarotae, cf. Alligatorium, Metriorhynchus sp.). Pterosauria (Rhamphorhynchus sp., Pterodactylus sp.). Dinosauria: Theropoda (Ceratosaurus sp. , Torvosaurus sp., Lourinhanosaurus antunesi, Allosaurus europaeus, Cf. Compsognathus sp., cf. Richardoestesia sp., Dromaeosaurinae indeter., Velociraptorinae indeter., cf. Archaeopteryx sp., aff. Paronychodon). Dinosauria: Sauropoda: Eusauropoda (Dinheirosaurus lourinhanensis, Lourinhasaurus alenquerensis, Lusotitan atalaiensis, Apatosaurus sp.). Dinosauria: Ornithischia: Thyreophora (Dacentrurus armatus, Stegosaurus sp., Dracopelta zbyszewskii). Dinosauria: Ornithischia: Ornithopoda (Phyllodon henkeli, Dryosaurus sp., Hypsilophodon sp., Alocodon kuehnei, Trimucrodon cuneatus, Draconyx loureiroi).

Background: Often, claims in Component-Based Development (CBD) are only sup-
ported by qualitative expert opinion, rather than by quantitative data. This contrasts
with the normal practice in other sciences, where a sound experimental validation of
claims is standard practice. Experimental Software Engineering (ESE) aims to bridge
this gap. Unfortunately, it is common to find experimental validation efforts that are
hard to replicate and compare, to build up the body of knowledge in CBD.

Objectives: In this dissertation our goals are (i) to contribute to evolution of ESE, in
what concerns the replicability and comparability of experimental work, and (ii) to ap-
ply our proposals to CBD, thus contributing to its deeper and sounder understanding.

Techniques: We propose a process model for ESE, aligned with current experimen-
tal best practices, and combine this model with a measurement technique called
Ontology-Driven Measurement (ODM). ODM is aimed at improving the state of prac-
tice in metrics definition and collection, by making metrics definitions formal and ex-
ecutable, without sacrificing their usability. ODM uses standard technologies that can
be well adapted to current integrated development environments.

Results: Our contributions include the definition and preliminary validation of a pro-
cess model for ESE and the proposal of ODM for supporting metrics definition and
collection in the context of CBD. We use both the process model and ODM to perform
a series experimental works in CBD, including the cross-validation of a component
metrics set for JavaBeans, a case study on the influence of practitioners expertise in
a sub-process of component development (component code inspections), and an ob-
servational study on reusability patterns of pluggable components (Eclipse plug-ins).
These experimental works implied proposing, adapting, or selecting adequate ontolo-
gies, as well as the formal definition of metrics upon each of those ontologies.

Limitations: Although our experimental work covers a variety of component models
and, orthogonally, both process and product, the plethora of opportunities for using
our quantitative approach to CBD is far from exhausted.

Conclusions: The main contribution of this dissertation is the illustration, through
practical examples, of how we can combine our experimental process model with
ODM to support the experimental validation of claims in the context of CBD, in a re-
peatable and comparableway. In addition, the techniques proposed in this dissertation
are generic and can be applied to other software development paradigms.

Software transactional memory (STM) is a promising programming model that adapts many concepts borrowed from the databases world to control concurrent accesses to memory (RAM) locations. In this paper we propose a new classification for the active states of a transaction; a new memory quiescing algorithm, to allow the safe transition of a memory block form transactional to non-transactional space; we compare word and object transactional grain units; and evaluate the cost of consistent state validation, arguing that this cost can be minimized by performing partial validation on problematic code regions.

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The characterization of the bottom ashes produced by two Portuguese municipal solid waste incinerators (MSWI) was performed with the aim of assessing the feasibility of using this waste as raw material in the production of glass that can be further processed as glass-ceramics for application in construction. Density and particle size distribution measurements were carried out for physical characterization. Chemical characterization revealed that SiO2, a network glass former oxide, was present in a relatively high content (52-58 wt%), indicating the suitability for this waste to be employed in the development of vitreous materials. CaO, Na2O and K2O, which act as fluxing agents, were present in various amounts (2-17 wt%) together with several other oxides normally present in ceramic and glass raw materials. Mineralogical characterization revealed that the main crystalline phases were quartz (SiO2) and calcite (CaCO3) and that minor amounts of different alkaline and alkaline-earth aluminosilicate phases were also present. Thermal characterization showed that the decomposition of the different compounds occurred up to 1100 °C and that total weight loss was <10 wt%. Heating both bottom ashes at 1400 °C for 2 h resulted in a melt with suitable viscosity to be poured into a mould, and homogeneous black-coloured glasses with a smooth shiny surface were obtained after cooling. The vitrified bottom ashes were totally amorphous as confirmed by X-ray diffraction. The results from the present experimental work indicate that the examined bottom ashes can be a potential material to melt and to obtain a glass that can be further processed as glass-ceramics to be applied in construction. © 2007 Elsevier Ltd. All rights reserved.

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Nanotechnology is having a positive impact on nearly every industry, and in particular in healthcare, where it is extending the limits of molecular diagnostics to the nanoscale-nanodiagnostics. Here we describe an innovative optoelectronic platform for the colorimetric detection of nucleic acids based on oligonucleotide-derivatized gold nanoparticles. The device integrates an amorphous/nanocrystalline biosensor and a light emission source with a gold nanoprobe for specific DNA detection. This low cost, fast and simple optoelectronic platform permits detection of few picomole of nucleic acid without target or signal amplification making it suitable for application in population diagnostics and in point-of-care hand-held devices. © 2007 Elsevier B.V. All rights reserved.

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This paper presents an analytical model for CMOS delay and power estimation in deep sub micrometer technologies. In this paper the EKV transistor model is considered as a way of granting the accuracy of results in the characterization of deep submicron CMOS circuits. The analytical model proposed is valid for a ramp input signal, and takes into account all the operation regions of the transistor as well the influence of the gate-to-drain capacitance. For estimating the power consumption, a simple numerical integration process is applied to the current wave. An application example considering the use of the model for the evaluation of the delay and power consumption associated to a CMOS inverter is considered. The validity of the results obtained with the proposed model for a 1.2V TSMCN65 CMOS inverter is checked against those obtained through Hspice simulation.