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Nitric Oxide Reductase (NOR) is an integral membrane protein performing the reduction of NO to N(2)O. NOR is composed of two subunits: the large one (NorB) is a bundle of 12 transmembrane helices (TMH). It contains a b type heme and a binuclear iron site, which is believed to be the catalytic site, comprising a heme b and a non-hemic iron. The small subunit (NorC) harbors a cytochrome c and is attached to the membrane through a unique TMH. With the aim to perform structural and functional studies of NOR, we have immunized dromedaries with NOR and produced several antibody fragments of the heavy chain (VHHs, also known as nanobodies (TM)). These fragments have been used to develop a faster NOR purification procedure, to proceed to crystallization assays and to analyze the electron transfer of electron donors. BIAcore experiments have revealed that up to three VHHs can bind concomitantly to NOR with affinities in the nanomolar range. This is the first example of the use of VHHs with an integral membrane protein. Our results indicate that VHHs are able to recognize with high affinity distinct epitopes on this class of proteins, and can be used as versatile and valuable tool for purification, functional study and crystallization of integral membrane proteins.

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In this paper, a poly-phase disc motor innovative feeding and control strategy, based on a variable poles approach, and its application to a high temperature superconductor (HTS) disc motor, are presented. The stator windings may be electronically commutated to implement a 2, 4, 6 or 8 poles winding, thus changing the motor's torque?speed characteristics. The motor may be a conventional induction motor with a conductive disc rotor, or a new HTS disc motor, with conventional copper windings at its two iron semi-stators and a HTS disc as a rotor. The conventional induction motor's operation principle is related with the induced electromotive forces in the conductive rotor. Its behaviour, characteristics and modelling through Steinmetz and others theories are well known. The operation principle of the motor with HTS rotor, however, is rather different and is related with vortices' dynamics and pinning characteristics; this is a much more complex process than induction, and its modelling is quite complicated. In this paper, the operation was simulated through finite-elements commercial software (FLUX2D), whereas superconductivity was simulated by the E-J power law. The electromechanical performance of both motor's computed are compared. Considerations about the systems overall efficiency, including cryogenics, are also discussed.

In this paper, a poly-phase disc motor innovative feeding and control strategy, based on a variable poles approach, and its application to a high temperature superconductor (HTS) disc motor, are presented. The stator windings may be electronically commutated to implement a 2, 4, 6 or 8 poles winding, thus changing the motor's torque?speed characteristics. The motor may be a conventional induction motor with a conductive disc rotor, or a new HTS disc motor, with conventional copper windings at its two iron semi-stators and a HTS disc as a rotor. The conventional induction motor's operation principle is related with the induced electromotive forces in the conductive rotor. Its behaviour, characteristics and modelling through Steinmetz and others theories are well known. The operation principle of the motor with HTS rotor, however, is rather different and is related with vortices' dynamics and pinning characteristics; this is a much more complex process than induction, and its modelling is quite complicated. In this paper, the operation was simulated through finite-elements commercial software (FLUX2D), whereas superconductivity was simulated by the E-J power law. The electromechanical performance of both motor's computed are compared. Considerations about the systems overall efficiency, including cryogenics, are also discussed.

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Os motores de histerese são muito atraentes, numa ampla gama de aplicações devido à característica binário - velocidade e simplicidade de construção. É esperado que o rendimento destes motores seja melhorado aquando do uso de materiais supercondutores de alta temperatura (SAT ? supercondutores de alta temperatura) e, de facto, os motores de histerese têm-se mostrado como, provavelmente, a melhor máquina eléctrica usando materiais SAT. Ambos os motores, quer convencional quer supercondutor (com SAT), são motores de histerese, mas apresentam diferentes fenómenos físicos para o seu funcionamento: o comportamento de histerese nos materiais ferromagnéticos convencionais é devido à falta de linearidade das suas propriedades magnéticas dos materiais ferromagnéticos enquanto a histerese nos materiais supercondutores de alta temperatura é de natureza ohmica e está relacionada com dinâmica de vórtices. Nesta tese aspectos teóricos, experimentais e simulados de ambos os motores são discutidos, realçando-se o princípio de funcionamento de cada um e as características mais relevantes de cada um. As características obtidas, quer por testes experimentais quer por uso do simulador usando elementos finitos (FLUX2D?), foram comparadas com o objectivo de avaliar o rendimento dos motores electromecânicos e a eficiência dos sistemas, incluindo a criogenia para os dispositivos supercondutores de alta temperatura.

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