Harold Widom proved in 1966 that the spectrum of a Toeplitz operator \(T(a)\) acting on the Hardy space \(H^p(\mathbb{T})\) over the unit circle \(\mathbb{T}\) is a connected subset of the complex plane for every bounded measurable symbol \(a\) and \(1 < p < \infty\). In 1972, Ronald Douglas established the connectedness of the essential spectrum of \(T(a)\) on \(H^2(\mathbb{T})\). We show that, as was suspected, these results remain valid in the setting of Hardy spaces \(H^p(\Gamma,w)\), \( 1 < p < \infty \), with general Muckenhoupt weights \(w\) over arbitrary Carleson curves \(\Gamma\).

Nitric Oxide Reductase (NOR) is an integral membrane protein performing the reduction of NO to N(2)O. NOR is composed of two subunits: the large one (NorB) is a bundle of 12 transmembrane helices (TMH). It contains a b type heme and a binuclear iron site, which is believed to be the catalytic site, comprising a heme b and a non-hemic iron. The small subunit (NorC) harbors a cytochrome c and is attached to the membrane through a unique TMH. With the aim to perform structural and functional studies of NOR, we have immunized dromedaries with NOR and produced several antibody fragments of the heavy chain (VHHs, also known as nanobodies). These fragments have been used to develop a faster NOR purification procedure, to proceed to crystallization assays and to analyze the electron transfer of electron donors. BIAcore experiments have revealed that up to three VHHs can bind concomitantly to NOR with affinities in the nanomolar range. This is the first example of the use of VHHs with an integral membrane protein. Our results indicate that VHHs are able to recognize with high affinity distinct epitopes on this class of proteins, and can be used as versatile and valuable tool for purification, functional study and crystallization of integral membrane proteins.

A instalação em larga escala de turbinas eólicas equipadas com um gerador de indução duplamente alimentado (GIDA) tem vindo a promover a realização de vários estudos relacionados com potenciais soluções para a sua integração na rede eléctrica. Nesta tese apresenta-se uma técnica de controlo que permite regular as potências activa e reactiva do GIDA de forma estável e independente. A sua viabilidade é suportada não só por um estudo teórico prévio mas também pelos resultados da simulação de modelos desenvolvidos usando o software Matlab?Simulink?. Apesar de este sistema funcionar a velocidade variável (ainda que dentro de certos limites), continua a existir a necessidade de limitar a potência fornecida pela turbina eólica quando a velocidade do vento assume valores superiores ao nominal. Assim, faz-se também uma breve análise de alguns métodos de regulação de potência activos e passivos.

The orange-coloured protein (ORP) from Desulfovibrio gigas is a 12 kDa protein that contains a novel mixed-metal sulfide cluster of the type [S(2)MoS(2)CuS(2)MoS(2)]. Diffracting crystals of the apo form of ORP have been obtained. Data have been collected for the apo form of ORP to 2.25 A resolution in-house and to beyond 2.0 A resolution at ESRF, Grenoble. The crystals belonged to a trigonal space group, with unit-cell parameters a = 43, b = 43, c = 106 A.

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A new faster control method is presented, inattempt to achieve stable operation and higher efficiency of anySeries Loaded Series Resonant (SLSR) power converter, andespecially when the application requires contactless energytransfer. This instantaneously reacting control method is basedon calculated individual energy portions delivered to theresonant circuit. Its viability is demonstrated by simulation of ananalogue circuit implementation.

THE CRETACEOUS SKELETON COAST OF ANGOLA JACOBS, Louis, SMU, Dallas, TX, USA; POLCYN, Michael, SMU, Dallas, TX, USA; MATEUS, Octávio, Museu da Lourinhã, Lourinhã, Portugal; SCHULP, Anne, Natuurhistorisch Museum Maastricht, Maastricht, Netherlands; NETO, André , Universidade Agostinho Neto, Luanda, Angola Cretaceous coastal sediments of Angola present a rich and diverse fauna of marine amniotes, including turtles, mosasaurs, and plesiosaurs. The abundance of mosasaurs in particular suggests a highly productive coastal area. Angola today lies at the northern limit of the Namibian Desert, the so-called Skeleton Coast, which results from prevailing southeasterly winds of the descending limb of the southern Hadley Cell sweeping across the African coast. The Benguela upwelling and a highly productive sea are found today off the Namibian Desert coast. However, the Benguela upwelling system, based on results of DSDP studies, is said to have originated in the late Neogene and therefore cannot explain the productivity found along the length of the West African coast. The explanation is found in the northward drift of Africa through the arid climate zone, and is demonstrated by the tracing of the paleogeographic position of fossil localities through time.

Analisa-se e caracteriza-se por via experimental a ligação entre elementos de betão armado e materiais compósitos, nomeadamente com base nas fibras de vidro. Fabricaram-se vigas de betão armado que foram exteriormente reforçadas com GFRP. Os resultados obtidos experimentalmente foram comparados com os resultados conseguidos por intermédio de modelação computacional, recorrendo-se ao programa de cálculo ATENA 2D. Para melhor modelação de elementos de interface, foram realizados ensaios de corte tendo-se obtido valores que permitiram caracterizar a lei de rotura de Mohr-Coulomb. Os parâmetros estudados foram a evolução das forças máximas absorvidas pelo reforço; as tensões de aderência máximas; a distribuição das tensões de aderência.

A mixture of 70% (w/w) pine biomass and 30% (w/w) plastics (mixture of polypropylene, polyethylene, and polystyrene) was subjected to pyrolysis at 400 °C, for 15 min, with an initial pressure of 40 MPa. Part of the solid residue produced was subjected to extraction with dichloromethane (DCM). The extracted residue (residue A) and raw residue (residue B) were analyzed by weight loss combustion and submitted to the leaching test ISO/TS 21268-2 using two different leachants: DCM (0.2%, v/v) and calcium chloride (0.001 mol/L). The concentrations of the heavy metals Cd, Cr, Ni, Zn, Pb and Cu were determined in the eluates and in the two residues. The eluates were further characterized by determining their pH and the concentrations of benzene, toluene, ethylbenzene and xylenes (BTEX). The presence of other organic contaminants in the eluates was qualitatively evaluated by gas chromatography, coupled with mass spectrometry. An ecotoxicological characterization was also performed by using the bio-indicator Vibrio fischeri. The chemical and ecotoxicological results were analyzed according to the French proposal of Criteria on the Evaluation Methods of Waste Ecotoxicity (CEMWE). Residue A was not considered to be ecotoxic by the ecotoxicological criterion (EC50 (30 min) ≥10%), but it was considered to be ecotoxic by the chemical criterion (Ni ≥ 0.5 mg/L). Residue B was considered to be ecotoxic by the ecotoxicological criterion: EC50 (30 min) ≤ 10%. Besides that, residue B was considered to be hazardous according the European legislation (BTEX concentrations higher than 100 ppb). The results indicate that volatile organic contaminants can be present in sufficient amounts in these residues and their eluates to induce ecotoxicity levels. The extraction of the pyrolysis residue with DCM was an efficient method for removing lighter organic contaminants.

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The co-combustion of sewage sludge (SS) and coal is widely used for the treatment and thermal valorization of SS produced in wastewater treatment plants. The chemical and ecotoxicological properties of the ashes produced in this thermal treatment have not been fully studied. Two combustion tests were performed in a fluidized bed combustor. Colombian coal was used as fuel in test A. A blend (1 + 1) of this coal and a stabilized SS (Biogran®) was used in a second test B. Samples of the bottom and fly ashes trapped in two sequential cyclones were collected. The characterization of the ashes was focused on two main aspects: (1) the bulk content of a set of metals and (2) the characterization of eluates produced according to the European Standard leaching test EN 12457-2. The eluates were submitted to an ecotoxicological characterization for two bio-indicators. In what concerns the bulk content of ashes, both combustion tests have produced ashes with different compositions. The ashes formed during the co-combustion test have shown higher concentrations of metals, namely Cr, Cu, Ni, Pb, Zn and Fe for all ashes. The leaching test has shown low mobility of these elements from the by-products produced during the combustion and co-combustion tests. Cr and Cr(VI) were mainly detected in the eluates of the 1st cyclone ashes produced in both combustion tests and in the 2nd cyclone ashes produced in the co-combustion test.

Considering the ecotoxicity assays, the eluates of bottom and fly ashes for both combustion and co-combustion tests have shown low ecotoxic levels. The micro-crustacean Daphnia magna was generally more sensitive than the bacterium Vibrio fischeri. CEMWE criterion has allowed to classify the bottom ashes for both combustion and co-combustion tests as non-toxic residues and the fly ashes collected in both cyclones as toxic.

The problem of finding the independent number of an undirected graph is formulated as two equivalent Mathematical Programs with Linear Complementarity Constraints (MPLCC). A multistarting Lemke’s method
is introduced for dealing with the first formulation and is able to find a good approximate of the independent
number in a finite number of iterations. A sequential complementary algorithm is also discussed for the second formulation and can find the independent number at least in theory. Some computational experience is included to highlight the efficacy of the complementary approaches for computing the independent number of graphs from the Dimacs collection.