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Tourism is an industry that has been growing rapidly in the last few years and it is expected that it will continue to grow. Due to the evolution of technology, mobile applications are being increasingly used in all kinds of industries, being one of them tourism. Presently there are already a few mobile applications used to increase the experience of the user when visiting a place, but these mobile applications lack some important features. This paper describes the development of a mobile application with integrated routing algorithms used to increase the experience of the tourists when visiting the city of Avila, Spain. The tourist will have at their disposal real time information about all the monuments available for visit, a full set of predefined circuits with different visit times and degrees of difficulty and also the possibility to create an optimized or personalized circuit combining the user preferences such as visiting time and number of monuments to visit.

A new and never yet reported hetero arylidene-9(10H)-anthrone structure (4) was unexpectedly isolated on reaction of 1,2-dimethyl-3-ethylimidazolium iodide (2) and 9-anthracenecarboxaldehyde (3) under basic conditions. Its structure was unequivocally attributed by X-ray crystallography. No cytotoxicity in human healthy fibroblasts and in two different cancer cell lines was observed indicating its applicability in biological systems. Compound 4 interacts with CT-DNA by intercalation between the adjacent base pairs of DNA with a high binding affinity (Kb = 2.0(± 0.20) x 105 M-1) which is 10x higher than that described for doxorubicin (Kb = 3.2 (±0.23) × 104 M-1). Furthermore, compound 4 quenches the fluorescence emission of GelRed-CT-DNA system with a quenching constant (KSV) of 3.3(±0.3) x 103 M-1 calculated by the Stern-Volmer equation.

Abstract Sandstones are widely used in the building elements of the world’s stone monuments. Alveolization due to salt crystallization-dissolution is the most important degradation pattern found on the Middle Ages’ sandstone façades of St. Leonardo’s Church in Portugal. An outstanding case of widespread distribution of deep and large alveolization patterns found mainly on portals and vaults of its sandstone façades appeared as a result of the past and present proximity of the seashore. On stonewall façades, a new approach of consolidating products’ treatments using the total head was followed in order to allow full absorption of stone. Physical and mechanical tests were carried out on selected representative specimens of varieties of sandstones on the monument. Treatments with ethyl silicates were assessed by means of its effectiveness, harmfulness, and durability. The comparison of results between the application of this new procedure on this stone monument and the traditional intervention treatments highlighted the potential viability of the former to overcome difficult conditions of absorption of consolidating products.

The p53 tumor suppressor is widely found to be mutated in human cancer. This protein is regarded as a molecular hub regulating different cell responses, namely cell death. Compelling data have demonstrated that the impairment of p53 activity correlates with tumor development and maintenance. For these reasons, the reactivation of p53 function is regarded as a promising strategy to halt cancer. In the present work, the recombinant mutant p53R280K DNA binding domain (DBD) was produced for the first time, and its crystal structure was determined in the absence of DNA to a resolution of 2.0 Å. The solved structure contains four molecules in the asymmetric unit, four zinc(II) ions, and 336 water molecules. The structure was compared with the wild-type p53 DBD structure, isolated and in complex with DNA. These comparisons contributed to a deeper understanding of the mutant p53R280K structure, as well as the loss of DNA binding related to halted transcriptional activity. The structural information derived may also contribute to the rational design of mutant p53 reactivating molecules with potential application in cancer treatment.