The compounds 1-naphthyliminoferrocene{,} 1{,} and 2-methyl-1-naphthyliminoferrocene{,} 2{,} were prepared by the reaction of ferrocenecarboxaldehyde and the corresponding amine{,} 1-naphthylamine and 2-methyl-1-naphthylamine with one drop of pyrrolidine as catalyst{,} in CH2Cl2{,} under inert atmosphere. The compounds were fully characterized by elemental analyses{,} matrix-assisted laser desorption/ionization - time-of-flight mass spectrometry (MALDI-TOF){,} FT-IR{,} 1H and 13C NMR as well as two-dimensional{,} NOESY{,} COSY and HSQC{,} NMR spectroscopy. Mossbauer spectroscopic studies were performed to characterize the iron species in both compounds. The solid-state molecular structures of compounds 1 and 2 were determined by single crystal X-ray diffraction. Analysis of the electrochemical behaviour of complexes 1 and 2 was performed by cyclic and square wave voltammetry. Compounds 1 and 2 were tested as chromogenic and electrochemical sensors for Hg2+.

Earthquakes, soil settlements, traffic vibrations, and air pollution are some of the actions that can affect historic old buildings. Besides these, the lack of continuous maintenance puts a large part of this heritage in risk due to structural problems that reduce their own safety and that of their users. The preservation and risk mitigation of built cultural heritage require the use of reliable tools in order to assess its state of conservation and to identify and prevent potential vulnerabilities. Having this in mind, it is not possible to carry out destructive tests in most historic old buildings, so it is preferable to opt for nondestructive tests (NDT) or alternative methodologies that allow the physical and mechanical characterization of materials and structure. In this chapter, a general view of NDT methods used in historic buildings to obtain the geometrical information, the damage mapping, the mechanical and physical characterization, and the petrographic analysis of stones is presented. An alternative methodology to physically and mechanically characterize the stone of historic buildings using NDT tests is also proposed. The chapter ends with a case study carried out in the St. Leonard’s Church, a Portuguese monument built in Atouguia da Baleia village in the 13th century, where the alternative methodology here presented was applied. The final results of this study show that the methodology proposed allows the obtention of stress-strain curves in a completely nondestructive way, based on the water absorption coefficient at low pressure.

Abstract The authors have recently proposed an innovative connector system that consists on a Glass Fibre Reinforced Polymer (GFRP) perforated plate that is embedded into Steel Fibre Reinforced Self-Compacting Concrete (SFRSCC) layers. The connection is strongly based in the mechanical interlock assured by the dowels originated from the \{SFRSCC\} passing through the holes opened on the \{GFRP\} plates. In this study, an analytical framework to evaluate the load capacity of the connections when loaded transversally was developed based on experimental pull-out tests presented in the companion paper (Part I). For a better understanding of the mechanical behaviour of the connections and to allow to make estimations of the load capacity of connection when it is conditioned by the rupture of the connector itself, pull-out pin-bearing tests with single-hole plates were executed to assess the effect of the type of \{GFRP\} on the strain distribution in the vicinity of the holes until the failure, as well as the estimated failure modes and load capacities of the connections.

The enhanced mechanical performance of thin-ply laminates results from their ability to delay the onset of damage typically observed in composite materials. However, in notched structures, subcritical damage growth causes beneficial stress redistributions in the vicinity of the notch, blunting the stress concentration. Precluding these damage mechanisms, as in thin-ply laminates, may potentially lead to inferior notched responses. To obviate this limitation of thin-ply laminates, a strategy based on the combination of standard grade 0� plies and thin transverse and off-axis plies is analysed in this paper. A detailed study of the effect of 0� ply blocking is carried out, with particular emphasis on the blunting mechanisms and notched response. Tests on scaled notched panels loaded in tension, with notch sizes between 6?mm and 30?mm, show that the combination of standard grade 0� ply blocks with thin transverse and off-axis plies promotes localised fibre-matrix splitting, which acts as an important notch blunting mechanism, while preventing matrix cracking and delamination. This results in an improved notched response and superior large damage capability. It is also shown that thicker 0� ply blocks provide higher stability in composite bolted joints, while the thin transverse and off-axis plies contribute for matrix-dominated damage suppression, resulting in an improved bolt-bearing response. The improvements of the large damage capability and bolt-bearing performance are obtained without compromising the superior unnotched tensile and compressive strengths intrinsic to thin-ply laminates.

The functional and biological significance of the selected CASP12 targets are described by the authors of the structures. The crystallographers discuss the most interesting structural features of the target proteins and assess whether these features were correctly reproduced in the predictions submitted to the CASP12 experiment. This article is protected by copyright. All rights reserved.

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It is known that zinc alloys with iron group metals have better corrosion resistance than pure zinc. Owing to the corrosion resistance of these alloys, Zn–Fe coatings are widely used in automotive industry...

Deconstruction of cellulose, the most abundant plant cell wall polysaccharide, requires the cooperative activity of a large repertoire of microbial enzymes. Modular cellulases contain non-catalytic type A Carbohydrate-Binding Modules (CBMs) that specifically bind to the crystalline regions of cellulose, thus promoting enzyme efficacy through proximity and targeting effects. Although type A CBMs play a critical role in cellulose recycling, their mechanism of action remains poorly understood. Here we produced a library of recombinant CBMs representative of the known diversity of type A modules. The binding properties of 40 CBMs, in fusion with an N-terminal green fluorescence protein (GFP) domain, revealed that type A CBMs possess the ability to recognize different crystalline forms of cellulose and chitin over a wide range of temperatures, pHs and ionic strengths. A Spirochaeta thermophila CBM64, in particular, displayed plasticity in its capacity to bind both crystalline and soluble carbohydrates under a wide range of extreme conditions. The structure of S. thermophila StCBM64C revealed an untwisted, flat, carbohydrate-binding interface comprising the side chains of four tryptophan residues in a coplanar linear arrangement. Significantly, two highly conserved asparagine side chains, each one located between two tryptophan residues, are critical to insoluble and soluble glucan recognition but not to bind xyloglucan. Thus, CBM64 compact structure and its extended and versatile ligand interacting platform illustrates how type A CBMs target their appended plant cell wall degrading enzymes to a diversity of recalcitrant carbohydrates under a wide range of environmental conditions.