Bras, Joana L. A., Ana Luisa Carvalho, Aldino Viegas, Shabir Najmudin, Victor D. Alves, Jose A. M. Prates, Luis M. A. Ferreira, Maria J. Romao, Harry J. Gilbert, and Carlos M. G. A. Fontes. "
Escherichia coli expression, purification, crystallization, and structure determination of bacterial cohesin-dockerin complexes."
Methods in enzymology. Vol. 510. 2012. 395-415.
AbstractCellulosomes are highly efficient nanomachines that play a fundamental role during the anaerobic deconstruction of complex plant cell wall carbohydrates. The assembly of these complex nanomachines results from the very tight binding of repetitive cohesin modules, located in a noncatalytic molecular scaffold, and dockerin domains located at the C-terminus of the enzyme components of the cellulosome. The number of enzymes found in a cellulosome varies but may reach more than 100 catalytic subunits if cellulosomes are further organized in polycellulosomes, through a second type of cohesin-dockerin interaction. Structural studies have revealed how the cohesin-dockerin interaction mediates cellulosome assembly and cell-surface attachment, while retaining the flexibility required to potentiate catalytic synergy within the complex. Methods that might be applied for the production, purification, and structure determination of cohesin-dockerin complexes are described here. Copyright 2012 Elsevier Inc. All rights reserved.
Cano, Manuela, Susana Nogueira, Ana Luisa Papoila, F. Aguiar, Pedro Martins, J. Marques, Iolanda Caires, J. Martins, C. Pedro, P. Paixão, J. Rosado-Pinto, Paula Leiria-Pinto, Daniel Aelenei, Ana Mendes, João Teixeira, M. C. Proença, and Nuno Neuparth. "
Indoor Air Quality in Portuguese Children Day Care Centers ? ENVIRH Project."
The Second International Conference on Building Energy and Environment (COBEE). 978-0-9816881-9-0; 0-9816881-9-5. 2012.
Abstract
Pereira, Pedro, Helena Fino, Fernando Coito, and M. Ventim-Neves. "
Optimization-Based Design of Nano-CMOS LC-VCOs."
Doctoral Conference on Computing, Electrical and Industrial Systems, DoCEIS 2012. Eds. Luis Camarinha-Matos, Ehsan Shahamatnia, and Gonçalo Nunes. Vol. 372. IFIP Advances in Information and Communication Technology, 372. Costa de Caparica - Portugal: Springer Boston, 2012. 453-464.
AbstractThis paper introduces a variability-aware methodology for the design of LC-VCOs in Nano-CMOS technologies. The complexity of the design as well as the necessity for having an environment offering the possibility for exploring design trade-offs has led to the development of design methodologies based multi-objective optimization procedures yielding the generation of Pareto-optimal surfaces. The efficiency of the process is granted by using analytical models for both passive and active devices. Although physics-based analytical expressions have been proposed for the evaluation of the lumped elements, the variability of the process parameters is usually ignored due to the difficulty to formalize it into an optimization performance index. The usually adopted methodology of considering only optimum solutions for the Pareto surface, may lead to pruning quasi-optimal solutions that may prove to be better, should their sensitivity to process parameter variation be accounted for. In this work we propose starting by generating an extended Pareto surface where both optimum and quasi-optimum solutions are considered. Finally information on the sensitivity to process parameter variations, is used for electing the best design solution.
Zeballos, L. J., M. I. Gomes, A. P. Barbosa-Povoa, and A. Q. Novais. "
Optimum Design and Planning of Resilient and Uncertain Closed-Loop Supply Chains."
22nd European Symposium on Computer Aided Process Engineering. Eds. Ian David Lockhart Bogle, and Michael Fairweather. Vol. 30. Computer Aided Chemical Engineering, 30. London: Elsevier, 2012. 407-411.
AbstractThe design and planning of efficient supply chains (SC) is a major challenge that increases when the return of products has to be accounted for, the so-called closed-loop supply chains (CLSC). In the present work the effect of disruptions and modifications in the operating conditions of CLSCs are investigated on the basis of a 2-stage scenario based model previously developed by the authors. Metrics derived from graph theory are used, along with more conventional economic and operational indices. A discussion on the results obtained is presented to assess how the design and planning of the CLSC respond to these challenges and how these metrics may contribute to this objective.
Pereira, Pedro, Fernando Coito, and Helena Fino. "
PSO-Based Design of RF Integrated Inductor."
Doctoral Conference on Computing, Electrical and Industrial Systems, DoCEIS 2012. Eds. Luis Camarinha-Matos, Ehsan Shahamatnia, and Gonçalo Nunes. Vol. 372. IFIP Advances in Information and Communication Technology, 372. Costa de Caparica - Portugal: Springer Boston, 2012. 475-482.
AbstractThis paper addresses an optimization-based approach for the design of RF integrated inductors. The methodology presented deals with the complexity of the design problem by formulating it as a multi-objective optimization. The multi-modal nature of the underlying functions combined with the need to be able to explore design trade-offs leads to the use of niching methods. This allows exploring not only the best trade-off solutions lying on the Pareto-optimum surface but also the quasi-optimum solutions that would be otherwise discarded. In this paper we take advantage of the niching properties of lbest PSO algorithm using ring topology to devise a simple optimizer able to find the local-optima. For the efficiency of the process analytical models are used for the passive/active devices. In spite the use of physics-based analytical expressions for the evaluation of the lumped elements, the variability of the process parameters is ignored in the optimization stage due to the significant computational burden it involves. Thus in the final stage both the Pareto-optimum solutions and the quasi-optimum solutions are evaluated with respect to the sensitivity to process parameter variations.