The paper is focused on the calibration of non-linear 3D finite element (FE) analyses to simulate punching failure of R/C flat slabs. The calibration procedure is developed with reference to the code ABAQUS, which is one of the most used computer codes in nonlinear modelling of R/C structures. Generally, the calibration of a nonlinear FE model is grounded on one test only, so its reliability could be limited. Here a hybrid method for the calibration of FE models of R/C flat slabs failing in punching is proposed and discussed. The method consists in calibrating input data by comparison of finite element model (FEM) results with both experimental data and predictions provided by analytical models. The procedure allows for a consistent calibration to be performed, valid for a wide range of longitudinal reinforcement ratios, from 0.5% to 2.00%, and concrete grades, from C20/25 to C50/60. A case study is investigated using the proposed method. Results show that calibrated values of the fracture energy lie between those provided by Model Code 1990 and Model Code 2010. From the new calibration procedure, a relationship between fracture energy and concrete compressive strength is also derived and blind analyses are performed to check its reliability against experimental results.

The aim of this study is to evaluate the elemental composition of six food supplements of plant origin, commonly sold in the Portuguese market, by energy dispersive X-ray fluorescence. The presence of arsenic in all the Maca, Ashwagandha, Camu-Camu and Hemp protein samples (except the generic form) is a reason of concern due to the long-term effects of As mainly in its inorganic form. Thus, great caution must be taken on some food supplements, particularly the cases of Moringa from Egypt and Yellow/Xpresso Maca, whose inorganic As concentrations are in line with the upper bound concentration for the 95th dietary exposure according to the European Food Safety Authority which is 0.64 μg/kg bw/day. In what regards Hemp protein, if the supplier’s daily intake recommendation (30 g) is followed, values as high as 1.75 μg/kg bw/day of inorganic As will be consumed, which are dangerously above the upper bound. In this case this specific supplement lot should be removed from the market. Also the consumption of Hemp protein leads to a daily intake of Mn above the Daily Reference Intake (DRI) and Adequate Intakes (AIs) for adults. The contamination of Goji berries by Pb is a reason for concern—organic berries contained 11.3 μg/g while berries derived from conventional agriculture 11.6 μg/g, leading to daily intake doses of 315.3 μg and 324.8 μg, respectively, if the recommended daily intake of 28 g is followed. Our findings point out to an inadequacy of the recommended intakes by the supplier vis a vis the concentrations observed, greatly increasing the risk for public health.

This paper deals with punching of reinforced high strength concrete (HSC) flat slabs. Despite the use of HSC increased significantly in the last years, the experimental research on punching behavior of HSC slabs is still limited. Furthermore, most of this past research adopted concrete compressive strength lower than 90 MPa. In a previous work by this research group three specimens with concrete compressive strength around 120 MPa and one with normal strength concrete (NSC) were tested. The present work represents the continuation of the previous activity and it is focused on the rational use of HSC. Four specimens with HSC and one of NSC were tested under monotonic vertical loading. The HSC was placed only in the slab-column connection region and it was limited to a thin layer in the compressive zone, in order to have a more economical and sustainable solution. This rational use of the HSC showed excellent results in terms of punching strength. Limiting the HSC to a thin layer in the compressive zone resulted in an almost equal punching strength to that obtained with the slab entirely casted in HSC.

{Two new bis(aryl-imino)-acenaphthene, Ar-BIAN (Ar = 2

The aim of the present paper is to evaluate the gravity loads effect in the behaviour of reinforced concrete beams critical zones subjected to cyclic loads. A numerical study to assess the influence of gravity load on RC beam critical zones subjected to cyclic loading is presented, assuming the level of gravity load as a variable parameter. For this purpose, the non-linear model was previously validated with an experimental campaign carried out on RC beam connection subject to cyclic loading with and without gravity loads. The consideration of the gravity load effects led to an accumulation of negative (hogging) deformation and the formation of an unidirectional plastic hinge. In order to validate this behaviour in an overall structural response, a non-linear numerical analysis of a RC frame system under cyclic loads, subject to different levels of gravity load, is also presented. The numerical results are analysed in terms of global hysterical response, accumulated energy dissipation and equivalent viscous damping ratio. In this numerical study was observed that the hysteretic response depends on the load path. In the presence of higher gravity loads levels, the structure hysteretic behaviour exhibits higher damage levels and the failure mechanism is prone to the formation of four plastic hinges. This phenomenon is analysed and discuss in the present paper. © Federation Internationale du Beton (fib) - International Federation for Structural Concrete, 2019.

The human natural killer-1 (HNK-1) epitope is a unique sulfated trisaccharide sequence presented on O- and N-glycans of various glycoproteins and on glycolipids. It is overexpressed in the nervous system and plays crucial roles in nerve regeneration, synaptic plasticity, and neuronal diseases. However, the investigation of functional roles of HNK-1 in a more complex glycan context at the molecular level remains a big challenge due to lack of access to related structurally well-defined complex glycans. Herein, we describe a highly efficient chemoenzymatic approach for the first collective synthesis of HNK-1-bearing O-mannose glycans with different branching patterns, and for their nonsulfated counterparts. The successful strategy relies on both chemical glycosylation of a trisaccharide lactone donor for the introduction of sulfated HNK-1 branch and substrate promiscuities of bacterial glycosyltransferases that can tolerate sulfated substrates for enzymatic diversification. Glycan microarray analysis with the resulting complex synthetic glycans demonstrated their recognition by two HNK-1-specific antibodies including anti-HNK-1/N-CAM (CD57) and Cat-315, which provided further evidence for the recognition epitopes of these antibodies and the essential roles of the sulfate group for HNK-1 glycan-antibody recognition.

One of the most promising approaches to produce industrial-compatible Transparent Conducting Materials (TCMs) with excellent characteristics is the fabrication of TCO/metal/TCO multilayers. In this article, we report on the electro-optical properties of a novel high-performing TCO/metal/TCO structure in which the intra-layer is a micro-structured metallic grid instead of a continuous thin film. The grid is obtained by evaporation of Ag through a mask of polystyrene colloidal micro-spheres deposited by the Langmuir-Blodgett method and partially dry-etched in plasma. IZO/Ag grid/IZO structures with different thicknesses and mesh dimensions have been fabricated, exhibiting excellent electrical characteristics (sheet resistance below 10 $Ømega$/□) and particularly high optical transmittance in the near-infrared spectral region as compared to planar (unstructured) TCM multilayers. Numerical simulations were also used to highlight the role of the Ag mesh parameters on the electrical properties.

The aim of the present study was to proceed to a numerical analysis of the gravity load effects on the behaviour of reinforced concrete beam critical zones when subjected to cyclic loads. A parametric study to assess the influence of different levels of gravity load on RC beam critical zones subjected to cyclic loading was carried out. For this purpose, assuming the level of gravity load as a variable parameter, a nonlinear numerical model of a beam-column connection, previously calibrated with experimental data, was used. In order to evaluate the gravity load effects in the global response, a numerical study of a RC frame system is also presented. The numerical results are analysed in terms of global hysteretic response, accumulated energy dissipation and equivalent viscous damping ratio. In this numerical study, it was observed that the hysteretic response depends on the load path. In the presence of higher gravity load levels, the structure hysteretic behaviour exhibits higher damage levels, associated to a failure mechanism corresponding to the formation of four span plastic hinges. Thus, the proper test procedure should involve the imposition of a reverse cyclic displacement history starting each cycle from the gravity load effects.

Warm mix asphalt (WMA) is produced by a variety of technologies at lower temperatures that enable to gain important environmental and social benefits and, in consequence, to contribute to a more sustainable transportation infrastructure. The producer usually defines the temperatures used in WMA production. However, some references suggest the increase of temperature in order to allow a longer time to transport or to compact in cases of unfavourable weather conditions, mainly under low temperatures. The objective of this paper is to analyse the feasibility of producing WMA at the same temperatures of hot mix asphalt (HMA) guaranteeing an adequate compaction, and final performance in service. The paper describes a laboratory study to investigate the properties of a WMA using different additives and varying the mixing and compaction temperatures. The effect of production temperatures on the performance of the WMA was evaluated through binder drainage (production phase), volumetric properties (compaction phase), and resistance to permanent deformation (service phase). The paper also presents a numerical study on the time available for paving WMA under unfavourable climatic conditions. Results demonstrated that it is possible to produce WMA at high temperatures without problems of binder drainage, during transport, and of performance in service if adequate compaction is achieved. In fact, the production temperatures influenced the compaction phase. However, it is possible to increase the temperature without negatively affecting the required volumetric properties. The rut depth of the permanent deformation test was mostly influenced by the air-voids of the compacted WMA and the binder. From the numerical study, it was concluded that the time available for in situ compaction increased substantially when WMA was paved at higher temperature. However, in cases of low air temperature and thin layer, the increase of temperature may not be sufficient to obtain the desired level of density or air-voids.

Ao longo da sua vida útil os edifícios históricos estão sujeitos a alterações de uso, a agentes ambientais e a diferentes ações como assentamentos do solo, incêndios, inundações ou sismos, para os quais podem não estar preparados. Além disso, a falta de manutenção contínua ajuda a colocar grande parte desse património em risco devido a problemas estruturais que reduzem sua própria segurança e a dos seus utilizadores. A preservação e mitigação de riscos do património cultural construído requer o uso de ferramentas confiáveis, a fim de avaliar o seu estado de conservação e identificar e prevenir potenciais vulnerabilidades. Os testes destrutivos tradicionais não são possíveis de realizar na maioria dos edifícios históricos, por isso é necessário selecionar testes não destrutivos (NDT) que permitam a caracterização física e mecânica dos materiais e do comportamento da estrutura. Neste artigo apresenta-se uma visão geral de diferentes equipamentos e testes NDT que permitem o levantamento geométrico e o mapeamento dos danos do edifício, a análise petrográfica da pedra de alvenaria, a caracterização das propriedades físicas e mecânicas dos materiais e o comportamento estrutural do edifício.

Os edifícios históricos como os palácios, castelos, igrejas e templos, são exemplos do património construído e constituem uma parte importante do nosso património cultural. Estas construções históricas incluem normalmente paredes de alvenaria maciça e pedra natural. A salvaguarda do património construído em pedra é de importância inquestionável, pelo que devem ser desenvolvidas e estudadas técnicas de intervenção adequadas em materiais e estruturas. Neste artigo, é apresentada uma metodologia de ensaios não destrutivos para avaliar a conservação de edifícios históricos de pedra e, com base nesta metodologia, é mostrado um caso de estudo na Igreja de São Leonardo, monumento português construído na Atouguia da Baleia no século XIII.

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Abstract An experimental work on reinforced concrete flat slab specimens to test the efficiency of postinstalled bolts, as punching shear reinforcement in resisting vertical and cyclic horizontal loads, was conducted and is presented in this paper. The test protocol consisted in increasing horizontal drifts combined with constant vertical load until failure. Two different detailing solutions for the shear reinforcement were considered, one using a radial distribution around the column and another using a cross distribution, being the results compared with a previously tested reference specimen. The dimensions of the specimens were 4.25 x 1.85 x 0.15 m3. The test setup used for these tests was developed by the research team and simulates the boundary conditions with already recognized good results. Postinstalled steel bolts were proven to be an efficient solution for strengthening of existing structures, improving the structural behavior, and the punching resistance.

Abstract An experimental work on reinforced concrete flat slab specimens to test the efficiency of postinstalled bolts, as punching shear reinforcement in resisting vertical and cyclic horizontal loads, was conducted and is presented in this paper. The test protocol consisted in increasing horizontal drifts combined with constant vertical load until failure. Two different detailing solutions for the shear reinforcement were considered, one using a radial distribution around the column and another using a cross distribution, being the results compared with a previously tested reference specimen. The dimensions of the specimens were 4.25 x 1.85 x 0.15 m3. The test setup used for these tests was developed by the research team and simulates the boundary conditions with already recognized good results. Postinstalled steel bolts were proven to be an efficient solution for strengthening of existing structures, improving the structural behavior, and the punching resistance.