Cellulosomes are highly sophisticated molecular nanomachines that participate in the deconstruction of complex polysaccharides, notably cellulose and hemicellulose. Cellulosomal assembly is orchestrated by the interaction of enzyme-borne dockerin (Doc) modules to tandem cohesin (Coh) modules of a non-catalytic primary scaffoldin. In some cases, as exemplified by the cellulosome of the major cellulolytic ruminal bacterium Ruminococcus flavefaciens, primary scaffoldins bind to adaptor scaffoldins that further interact with the cell surface via anchoring scaffoldins, thereby increasing cellulosome complexity. Here we elucidate the structure of the unique Doc of R. flavefaciens FD-1 primary scaffoldin ScaA, bound to Coh 5 of the adaptor scaffoldin ScaB. The RfCohScaB5-DocScaA complex has an elliptical architecture similar to previously described complexes from a variety of ecological niches. ScaA Doc presents a single-binding mode, analogous to that described for the other two Coh-Doc specificities required for cellulosome assembly in R. flavefaciens. The exclusive reliance on a single-mode of Coh recognition contrasts with the majority of cellulosomes from other bacterial species described to date, where Docs contain two similar Coh-binding interfaces promoting a dual-binding mode. The discrete Coh-Doc interactions observed in ruminal cellulosomes suggest an adaptation to the exquisite properties of the rumen environment.

Overexpression of the Thomsen-Friedenreich (TF) antigen in cell membrane proteins occurs in 90% of adenocarcinomas. Additionally, the binding of the TF-antigen to human galectin-3 (Gal-3), also frequently overexpressed in malignancy, promotes cancer progression and metastasis. In this context, structures that interfere with this specific interaction display the potential to prevent cancer metastasis. Herein, a multidisciplinary approach, combining the optimized synthesis of a TF-antigen mimetic with NMR, X-ray crystallography methods and isothermal titration calorimetry assays has been employed to unravel the molecular structural details that govern the Gal-3/TF-mimetic interaction. The TF-mimetic presents a binding affinity for Gal-3 similar to the TF-natural antigen and retains the binding epitope and the bioactive conformation observed for the native antigen. Furthermore, from a thermodynamic perspective a decrease in the enthalpic contribution was observed for the Gal-3/TF-mimetic complex, however this behaviour is compensated by a favourable entropy gain. From a structural perspective, these results establish our TF-mimetic as a scaffold to design multivalent solutions to potentially interfere with Gal-3 aberrant interactions and likely be used to hamper Gal-3-mediated cancer cells adhesion and metastasis.

A produção de betão com substituição de agregados naturais por agregados reciclados minimiza os impactes da indústria da construção. Contudo, o recurso a este tipo de agregado é limitado devido a dúvidas dos diferentes agentes da indústria da construção e à ausência de regulamentação específica para o projecto de betão com agregados reciclados. A percepção de betões com agregados reciclados como um material heterogéneo com comportamento imprevisível é o principal entrave, quer para a aceitação da indústria, quer para o desenvolvimento de regulamentação. Diferentes aspectos relacionados com o efeitos da variabilidade das propriedades de betão com agregados reciclados na regulamentação de estruturas são abordados conceptualmente: a heterogeneidade dos agregados reciclados, o efeito desta heterogeneidade nas propriedades mecânicas de betão e os efeitos da variabilidade das propriedades de betão na sua fiabilidade estrutural. Os primeiros resultados de uma campanha experimental desenvolvida especificamente para abordar o efeito de agregados grossos reciclados na fiabilidade são apresentados e as suas implicações no projecto de estruturas discutidas. São apresentadas sugestões de desenvolvimentos futuros que visam a calibração de coeficientes parciais de segurança que possibilitem o projecto de estruturas de betão armado dimensionadas segundo o formato do Eurocódigo 2 e mantendo os mesmos níveis de segurança de betão convencional.

Pseudo-cyclic and cyclic loading were applied to CFRP-to-steel bonded joints built with two different CFRP laminates. In this paper, the strength capacity and bond-slip curves are presented and compared. The modes of failure are also described and associated with the types of material used, and the observed performances are correlated. The analysis of the results showed a threshold value for loading and amplitude level, below which the cyclic loading caused no detectable damage. For cycles above that limit, the region of the joints around the loaded end presented degradation reflected on the bond-slip stiffness and on the increase of residual deformation. It was found that the normalized dissipated energies either obtained from the bond-slip relationship or from the load-slip response had the same trend. The experimental data allowed also to establish a relationship between the damage developed within the interface and the normalized slip. A preliminary estimate of fatigue limit based on those data is suggested.

This paper presents a wireless energy transfer (WET) system operating at the frequency of tens of kHz. It treats the modeling and simulation of WET prototype and its comparison with experimental measuring results. The wireless energy transfer system model was created to simulate the electric field between the emitting and the receiving coils, applying the finite element method. The results from the simulation are compared to the measured values of the electric field emission from the wireless energy transfer equipment. In the recent years the interest in the WET technology, especially for the electric vehicles (EV) batteries charging, is rapidly growing. The WET systems pollute the environment by electromagnetic emissions. Due to the expanding use of this technology in industrial and consumer electronics products, the problems associated with the electromagnetic compatibility (EMC), and the adverse impact on the human health becomes highly important.

This paper presents the experimental results obtained in the wireless energy transfer (WET) system prototype based on coils: circular or planar. With these experimental results we can choose the tuning settings to improve the efficiency of power transmission of the WET systems. In WET for electric vehicle batteries charging, the coil shape and the range between the coils are the most important issues of those systems.

n/a