Earthquakes, soil settlements, traffic vibrations, and air pollution are some of the actions that can affect historic old buildings. Besides these, the lack of continuous maintenance puts a large part of this heritage in risk due to structural problems that reduce their own safety and that of their users. The preservation and risk mitigation of built cultural heritage require the use of reliable tools in order to assess its state of conservation and to identify and prevent potential vulnerabilities. Having this in mind, it is not possible to carry out destructive tests in most historic old buildings, so it is preferable to opt for nondestructive tests (NDT) or alternative methodologies that allow the physical and mechanical characterization of materials and structure. In this chapter, a general view of NDT methods used in historic buildings to obtain the geometrical information, the damage mapping, the mechanical and physical characterization, and the petrographic analysis of stones is presented. An alternative methodology to physically and mechanically characterize the stone of historic buildings using NDT tests is also proposed. The chapter ends with a case study carried out in the St. Leonard’s Church, a Portuguese monument built in Atouguia da Baleia village in the 13th century, where the alternative methodology here presented was applied. The final results of this study show that the methodology proposed allows the obtention of stress-strain curves in a completely nondestructive way, based on the water absorption coefficient at low pressure.

Abstract The family 81 glycoside hydrolase (GH81) from Clostridium thermocellum is a β-1,3-glucanase belonging to cellulosomal complex. The gene encoding \{GH81\} from Clostridium thermocellum (CtLam81A) was cloned and expressed displaying a molecular mass of  82 kDa. CtLam81A showed maximum activity against laminarin (100 U/mg), followed by curdlan (65 U/mg), at pH 7.0 and 75 °C. CtLam81A displayed Km, 2.1 ± 0.12 mg/ml and Vmax, 109 ± 1.8 U/mg, against laminarin under optimized conditions. CtLam81A activity was significantly enhanced by Ca2+ or Mg2+ ions. Melting curve analysis of CtLam81A showed an increase in melting temperature from 91 °C to 96 °C by Ca2+ or Mg2+ ions and decreased to 82 °C by EDTA, indicating that Ca2+ and Mg2+ ions may be involved in catalysis and in maintaining structural integrity. \{TLC\} and MALDI-TOF analysis of β-1,3-glucan hydrolysed products released initially, showed β-1,3-glucan-oligosaccharides degree of polymerization (DP) from \{DP2\} to DP7, confirming an endo-mode of action. The catalytically inactive mutant CtLam81A-E515A generated by site-directed mutagenesis was co-crystallized and tetragonal crystals diffracting up to 1.4 Å resolution were obtained. CtLam81A-E515A contained 15 α-helices and 38 β-strands forming a four-domain structure viz. a β-sandwich domain I at N-terminal, an α/β-domain II, an (α/α)6 barrel domain III, and a small 5-stranded β-sandwich domain IV.

Based on a few experimental results available in the literature, this work presents a simple analytical approach that allows the study of the long-term behaviour of CFRP-to-concrete interfaces under an initial sustaining load. Only the elastic regime is studied, which means that the interfacial maximum bond stress and maximum slip are never exceeded. Therefore, the maximum initial load to be sustained by the joints is limited by its corresponding elastic value. The analytical results provided by the proposed model are compared with some experimental results found in the literature. The results showed strain redistribution throughout the bonded length over the time.

The durability performance of stainless steel makes it an interesting alternative for the structural strengthening of reinforced concrete. Like external steel plates or fibre reinforced polymers, stainless steel can be applied using externally bonded reinforcement (EBR) or the near surface mounted (NSM) bonding techniques. In the present work, a set of single-lap shear tests were carried out using the EBR and NSM bonding techniques. The evaluation of the performance of the bonding interfaces was done with the help of the digital image correlation (DIC) technique. The tests showed that the measurements gathered with DIC should be used with caution, since there is noise in the distribution of the slips and only the slips greater than one-tenth of a millimetre were fairly well predicted. For this reason, the slips had to be smoothed out to make it easier to determine the strains in the stainless steel and the bond stress transfer between materials, which helps to determine the bond–slip relationship of the interface. Moreover, the DIC technique allowed to identify all the states developed within the interface through the load–slip responses which were also closely predicted with other monitoring devices. Considering the NSM and the EBR samples with the same bonded lengths, it can be stated that the NSM system has the best performance due to their higher strength, being observed the rupture of the stainless steel in the samples with bond lengths of 200 and 300 mm. Associated with this higher strength, the NSM specimens had an effective bond length of 168 mm which is 71.5% of that obtained for the EBR specimens (235 mm). A trapezoidal and a power functions are the proposed shapes to describe the interfacial bond–slip relationships of the NSM and EBR systems, respectively, where the maximum bond stress in the former system is 1.8 times the maximum bond stress of the latter one. © 2018, The Author(s).

The enhanced mechanical performance of thin-ply laminates results from their ability to delay the onset of damage typically observed in composite materials. However, in notched structures, subcritical damage growth causes beneficial stress redistributions in the vicinity of the notch, blunting the stress concentration. Precluding these damage mechanisms, as in thin-ply laminates, may potentially lead to inferior notched responses. To obviate this limitation of thin-ply laminates, a strategy based on the combination of standard grade 0� plies and thin transverse and off-axis plies is analysed in this paper. A detailed study of the effect of 0� ply blocking is carried out, with particular emphasis on the blunting mechanisms and notched response. Tests on scaled notched panels loaded in tension, with notch sizes between 6?mm and 30?mm, show that the combination of standard grade 0� ply blocks with thin transverse and off-axis plies promotes localised fibre-matrix splitting, which acts as an important notch blunting mechanism, while preventing matrix cracking and delamination. This results in an improved notched response and superior large damage capability. It is also shown that thicker 0� ply blocks provide higher stability in composite bolted joints, while the thin transverse and off-axis plies contribute for matrix-dominated damage suppression, resulting in an improved bolt-bearing response. The improvements of the large damage capability and bolt-bearing performance are obtained without compromising the superior unnotched tensile and compressive strengths intrinsic to thin-ply laminates.

The plant cell-wall is constituted by structurally diverse polysaccharides. The biodegradation of these is a crucial process for life sustainability. Cellulolytic microorganisms are highly efficient in this process by assembling modular architectures of carbohydrate-active enzymes with appended non-catalytic carbohydrate-binding modules (CBMs). Carbohydrate microarrays offer high-throughput and sensitive tools for uncovering carbohydrate-binding specificities of CBMs{,} which is pivotal to understand the function of these modules in polysaccharide biodegradation mechanisms. Features of this technology will be here briefly reviewed with highlights of microarray approaches to study plant-carbohydrates and CBM-carbohydrate interactions{,} along with an overview of plant polysaccharides and microorganisms strategies for their recognition.

The effective stress transfer between the fiber reinforced polymers (FRP) and the steel substrate is crucial for the successful retrofit of existing steel structures with FRP composites. However, there are no standard tests for FRP-to-steel interfaces, wherefore different test configurations have been used in recent years to assess the bond behaviour in these interfaces. The present study shows that the choice of test configuration is highly important and leads to different transfer stresses between the FRP and steel composites and consequently, has a direct influence on the strength of the bonded joint. Therefore, it is important to understand the debonding process that occurs in each test and avoid misinterpretations, erroneous analyses and dangerous characterizations of the interfacial behaviour of these interfaces. The current study presents a new analytical approach for the prediction of the debonding of FRP-to-steel interfaces when double-lap pull or double-strap tests are used. © 2017 Elsevier Ltd

{The theory of turbulent Newtonian fluids shows that the choice of the boundary condition is a relevant issue because it can modify the behavior of a fluid by creating or avoiding a strong boundary layer. In this study, we consider stochastic second grade fluids filling a two-dimensional bounded domain with the Navier-slip boundary condition (with friction). We prove the well-posedness of this problem and establish a stability result. Our stochastic model involves a multiplicative white noise and a convective term with third order derivatives, which significantly complicate the analysis. (C) 2017 Elsevier Inc. All rights reserved.}

The functional and biological significance of the selected CASP12 targets are described by the authors of the structures. The crystallographers discuss the most interesting structural features of the target proteins and assess whether these features were correctly reproduced in the predictions submitted to the CASP12 experiment. This article is protected by copyright. All rights reserved.