The cyclic failure observed in structural components such as pipelines subjected to extreme loading conditions highlights some limitations concerning the application of existing fatigue damage models. The evaluation and prediction of this type of failure in these steel components under large-scale plastic yielding associated with high levels of stress triaxiality are not sufficiently known nor explored. This fatigue domain is conventionally called ultra-low-cycle fatigue (ULCF) and damage features are representative of both low-cycle fatigue (LCF) and monotonic ductile fracture. Thus, in order to understand the ULCF damage mechanisms both monotonic and LCF tests are required to get representative bounding damage information to model the material damage behaviour under such extreme loading conditions. This paper aims at exploring the Theory of Critical Distances (TCD) in the LCF and ULCF fatigue regimes, including the application of the point, line and area methods. The application of the TCD theories has not been explored so far in the ULCF fatigue regimes, despite its promising results in the LCF and high-cycle fatigue. An experimental program was carried out on several specimens’ geometries made of X52 piping steel. In detail, smooth plane specimens and notched plane specimens were cyclic loaded under tension/compression loading in order to obtain fatigue lives within the range of 101-104 cycles. In addition, cyclic bending tests on notched plane specimens were also incorporated in this study. Finite element simulations of all small-scale tests were conducted allowing to derive elastoplastic stress/strain fields along the potential crack paths. The numerical data were subjected to a post-processing in order to find characteristic lengths that can be treated as a fatigue property according to the TCD. A unified strain-life relation is proposed for the X52 piping steel together with a characteristic material length, consisting of a practical relation for pipeline strain-based design under extreme cyclic loading conditions.

This work studies the low-velocity impact response of 3D-printed layered structures made of thermoplastic materials (PLA and PETg), which form sacrificial claddings for impact protection. The analyzed structures are composed of crushable cellular cores placed in between terminal stiffening plates. The cores tessellate either honeycomb hexagonal unit cells, or hexagonal cells with re-entrant corners, with the latter exhibiting auxetic response. The given results highlight that the examined PETg protectors exhibit higher energy dissipation ratios and lower restitution coefficients, as compared to PLA structures that have the same geometry. It is concluded that PETg qualifies as an useful material for the fabrication of effective impact protection gear through ordinary, low-cost 3D printers.

The aim of this study is to evaluate the elemental composition of six food supplements of plant origin, commonly sold in the Portuguese market, by energy dispersive X-ray fluorescence. The presence of arsenic in all the Maca, Ashwagandha, Camu-Camu and Hemp protein samples (except the generic form) is a reason of concern due to the long-term effects of As mainly in its inorganic form. Thus, great caution must be taken on some food supplements, particularly the cases of Moringa from Egypt and Yellow/Xpresso Maca, whose inorganic As concentrations are in line with the upper bound concentration for the 95th dietary exposure according to the European Food Safety Authority which is 0.64 μg/kg bw/day. In what regards Hemp protein, if the supplier’s daily intake recommendation (30 g) is followed, values as high as 1.75 μg/kg bw/day of inorganic As will be consumed, which are dangerously above the upper bound. In this case this specific supplement lot should be removed from the market. Also the consumption of Hemp protein leads to a daily intake of Mn above the Daily Reference Intake (DRI) and Adequate Intakes (AIs) for adults. The contamination of Goji berries by Pb is a reason for concern—organic berries contained 11.3 μg/g while berries derived from conventional agriculture 11.6 μg/g, leading to daily intake doses of 315.3 μg and 324.8 μg, respectively, if the recommended daily intake of 28 g is followed. Our findings point out to an inadequacy of the recommended intakes by the supplier vis a vis the concentrations observed, greatly increasing the risk for public health.

Understanding the specific molecular interactions between proteins and β1,3-1,4-mixed-linked d-glucans is fundamental to harvest the full biological and biotechnological potential of these carbohydrates and of proteins that specifically recognize them. The family 11 carbohydrate-binding module from Clostridium thermocellum (CtCBM11) is known for its binding preference for β1,3-1,4-mixed-linked over β1,4-linked glucans. Despite the growing industrial interest of this protein for the biotransformation of lignocellulosic biomass, the molecular determinants of its ligand specificity are not well defined. In this report, a combined approach of methodologies was used to unravel, at a molecular level, the ligand recognition of CtCBM11. The analysis of the interaction by carbohydrate microarrays and NMR and the crystal structures of CtCBM11 bound to β1,3-1,4-linked glucose oligosaccharides showed that both the chain length and the position of the β1,3-linkage are important for recognition, and identified the tetrasaccharide Glcβ1,4Glcβ1,4Glcβ1,3Glc sequence as a minimum epitope required for binding. The structural data, along with site-directed mutagenesis and ITC studies, demonstrated the specificity of CtCBM11 for the twisted conformation of β1,3-1,4-mixed-linked glucans. This is mediated by a conformation–selection mechanism of the ligand in the binding cleft through CH-π stacking and a hydrogen bonding network, which is dependent not only on ligand chain length, but also on the presence of a β1,3-linkage at the reducing end and at specific positions along the β1,4-linked glucan chain. The understanding of the detailed mechanism by which CtCBM11 can distinguish between linear and mixed-linked β-glucans strengthens its exploitation for the design of new biomolecules with improved capabilities and applications in health and agriculture. Database Structural data are available in the Protein Data Bank under the accession codes 6R3M and 6R31.

An innovative system for the flexural strengthening of RC structures designated continuous reinforcement embedded at ends (CREatE) is presented in this research work. The main characteristics and procedures for the application of this new strengthening technique were described. To evaluate the performance and efficiency of this technique, a set of RC T-beams was subjected to a four-point bending test setup. The reference RC T-beam was not strengthened; all other RC T-beams were strengthened with postinstalled stainless steel bars. Different application arrangements and different amounts of reinforcement were considered, and the CREatE technique was tested under monotonic and cyclic loading histories. The tests were modeled using the nonlinear finite-element method (FEM) to predict the performance of the RC T-beams, which allowed analyzing, in detail and with good agreement with the experiments, the influence of the CREatE technique on the (1) strains developed in the concrete, (2) cracking patterns, and (3) strains developed in the stirrups. Apart from the expected increases in the flexural stiffness and load-bearing capacity of the T-beams, the results showed that the use of the CREatE technique led to higher ductility indexes in the displacement compared with traditional techniques. Moreover, with the CREatE technique, premature debonding of the reinforcement material from the concrete tensioned surface - commonly observed in externally bonded reinforcement (EBR) strengthening systems - was eliminated. © 2020 American Society of Civil Engineers.

{Two new bis(aryl-imino)-acenaphthene, Ar-BIAN (Ar = 2

Journal of Analytical Atomic Spectrometry (2020), 35, 2920-2927, doi:10.1039/D0JA00307G

Abstract Photonic front-coatings with self-cleaning properties are presented as means to enhance the efficiency and outdoor performance of thin-film solar cells, via optical enhancement while simultaneously minimizing soiling-related losses. This is achieved by structuring parylene-C transparent encapsulants using a low-cost and highly-scalable colloidal-lithography methodology. As a result, superhydrophobic surfaces with broadband light-trapping properties are developed. The optimized parylene coatings show remarkably high water contact angles of up to 165.6° and extremely low adhesion, allowing effective surface self-cleaning. The controlled nano/micro-structuring of the surface features also generates strong anti-reflection and light scattering effects, corroborated by numeric electromagnetic modeling, which lead to pronounced photocurrent enhancement along the UV?vis?IR range. The impact of these photonic-structured encapsulants is demonstrated on nanocrystalline silicon solar cells, that show short-circuit current density gains of up to 23.6%, relative to planar reference cells. Furthermore, the improvement of the devices' angular response enables an enhancement of up to 35.2% in the average daily power generation.

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