Cellulosomes are sophisticated multi-enzymatic nanomachines produced by anaerobes to effectively deconstruct plant structural carbohydrates. Cellulosome assembly involves the binding of enzyme-borne dockerins (Doc) to repeated cohesin (Coh) modules located in a non-catalytic scaffoldin. Docs appended to cellulosomal enzymes generally present two similar Coh-binding interfaces supporting a dual-binding mode, which may confer increased positional adjustment of the different complex components. Ruminococcus flavefaciens’ cellulosome is assembled from a repertoire of 223 Doc-containing proteins classified into 6 groups. Recent studies revealed that Docs of groups 3 and 6 are recruited to the cellulosome via a single-binding mode mechanism with an adaptor scaffoldin. To investigate the extent to which the single-binding mode contributes to the assembly of R. flavefaciens cellulosome, the structures of two group 1 Docs bound to Cohs of primary (ScaA) and adaptor (ScaB) scaffoldins were solved. The data revealed that group 1 Docs display a conserved mechanism of Coh recognition involving a single-binding mode. Therefore, in contrast to all cellulosomes described to date, the assembly of R. flavefaciens cellulosome involves single but not dual-binding mode Docs. Thus, this work reveals a novel mechanism of cellulosome assembly and challenges the ubiquitous implication of the dual-binding mode in the acquisition of cellulosome flexibility.

The Spheroolithidae oospecies Guegoolithus turolensis, putatively attributed to non-hadrosauroid styracosterns was first described in the Barremian of the Iberian Basin, and later reported in the Valanginian–Hauterivian of the Cameros Basin, with both occurrences separated by a few hundred kilometres but by over 10 million years.

ABSTRACTCyclotosaurus naraserluki, sp. nov., is a new Late Triassic capitosaurid amphibian from lacustrine deposits in the Fleming Fjord Formation of the Jameson Land Basin in Greenland. It is based on a fairly complete and well-preserved skull associated with two vertebral intercentra. Previously reported as Cyclotosaurus cf. posthumus, C. naraserluki is unique among cyclotosaurs for having the postorbitals embaying the supratemporals posteromedially. The anterior palatal vacuity presents an autapomorphic complete subdivision by a wide medial premaxillary-vomerine bony connection. The parasphenoid projects between the pterygoids and the exoccipitals, preventing a suture between the two, a primitive condition shared with Rhinesuchidae, Eryosuchus, and Kupferzellia. Within Cyclotosaurus, the Greenlandic skull has a distinctive combination of circular choanae (shared with C. ebrachensis, C. posthumus, and C. robustus) and a convex posteromedial margin of the tabulars (also present in C. ebrachensis and C. intermedius). A phylogenetic analysis indicates that C. naraserluki is the sister taxon of the middle Norian C. mordax from southern Germany, with which it shares a pair of premaxillary foramina. Cyclotosaurus is one of the most successful and diverse genera of Late Triassic temnospondyls, with at least eight species reported from middle Carnian to late Norian. Cyclotosaurus naraserluki is the largest amphibian ever reported from Greenland and one of the Late Triassic vertebrates with the highest northern paleolatitude currently known.http://zoobank.org/urn:lsid:zoobank.org:pub:43AAA541-031C-4EE1-B819-4846EBBD1BBBSUPPLEMENTAL DATA?Supplemental materials are available for this article for free at www.tandfonline.com/UJVPCitation for this article: Marzola, M., O. Mateus, N. H. Shubin, and L. B. Clemmensen. 2017. Cyclotosaurus naraserluki, sp. nov., a new Late Triassic cyclotosaurid (Amphibia, Temnospondyli) from the Fleming Fjord Formation of the Jameson Land Basin (East Greenland). Journal of Vertebrate Paleontology. DOI: 10.1080/02724634.2017.1303501.ABSTRACTCyclotosaurus naraserluki, sp. nov., is a new Late Triassic capitosaurid amphibian from lacustrine deposits in the Fleming Fjord Formation of the Jameson Land Basin in Greenland. It is based on a fairly complete and well-preserved skull associated with two vertebral intercentra. Previously reported as Cyclotosaurus cf. posthumus, C. naraserluki is unique among cyclotosaurs for having the postorbitals embaying the supratemporals posteromedially. The anterior palatal vacuity presents an autapomorphic complete subdivision by a wide medial premaxillary-vomerine bony connection. The parasphenoid projects between the pterygoids and the exoccipitals, preventing a suture between the two, a primitive condition shared with Rhinesuchidae, Eryosuchus, and Kupferzellia. Within Cyclotosaurus, the Greenlandic skull has a distinctive combination of circular choanae (shared with C. ebrachensis, C. posthumus, and C. robustus) and a convex posteromedial margin of the tabulars (also present in C. ebrachensis and C. intermedius). A phylogenetic analysis indicates that C. naraserluki is the sister taxon of the middle Norian C. mordax from southern Germany, with which it shares a pair of premaxillary foramina. Cyclotosaurus is one of the most successful and diverse genera of Late Triassic temnospondyls, with at least eight species reported from middle Carnian to late Norian. Cyclotosaurus naraserluki is the largest amphibian ever reported from Greenland and one of the Late Triassic vertebrates with the highest northern paleolatitude currently known.http://zoobank.org/urn:lsid:zoobank.org:pub:43AAA541-031C-4EE1-B819-4846EBBD1BBBSUPPLEMENTAL DATA?Supplemental materials are available for this article for free at www.tandfonline.com/UJVPCitation for this article: Marzola, M., O. Mateus, N. H. Shubin, and L. B. Clemmensen. 2017. Cyclotosaurus naraserluki, sp. nov., a new Late Triassic cyclotosaurid (Amphibia, Temnospondyli) from the Fleming Fjord Formation of the Jameson Land Basin (East Greenland). Journal of Vertebrate Paleontology. DOI: 10.1080/02724634.2017.1303501.

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The present paper aims to examine the potential of the Applied Element Method (AEM) in simulating the blast effects in RC panels. The numerical estimates are compared with the results obtained in an experimental campaign designed to investigate the effectiveness of fibre grout for strengthening full scale RC panels by comparing the effects that a similar blast load produces in a reference and the strengthened panel. First, a numerical model of the reference specimen was created in the software Extreme Loading for Structures and calibrated to match the experimental results. With no further calibration, the fibre reinforced grout strengthening was added and the resulting numerical model subjected to the same blast load. The experimental blast effects on both reference and strengthened panels, despite the lack of high speed measurement equipment (pressure, strains and displacements sensors), compare well with the numerical estimates in terms of residual and maximum displacements, showing that, once calibrated, the AEM numerical models can be successfully used to simulate blast effects in RC panels.

Fiber Reinforced Polymers (FRP) is a recent technique to strengthen timber structures and the studies available discussing the debonding between these materials are limited. Therefore, the bond assessment between FRP composites and timber substrates is a topic that needs clarification. The present work analyses the debonding process between Carbon (C) FRP laminates and timber with rupture modes consistent with Mode II interfacial fracture, i.e. with the sliding mode where the bond stresses act parallel to the plane of the bonding surface. Several single-lap shear tests were performed and the experiments showed a nonlinear local behaviour of the CFRP-to-timber interface. An interfacial bond-slip model and its calibration procedure were also presented. Furthermore, the calibrated nonlinear bond-slip model was implemented in a numerical approach where the FRP composite and the adhesive are simulated by linear and nonlinear springs and the substrate is assumed rigid. The following influences on the debonding process of the CFRP-to-timber interface were also analysed: (i) the bonding technique (Externally Bonded Reinforcement - EBR; and Near Surface Mounted - NSM); and (ii) the use of an additional device to mechanically anchor the CFRP laminate. Besides the determination of the effective bond length for each bonding technique, a new concept defining the length beyond which the force at the anchorage device does not decrease with the bonded length and a proposal to estimate its value for any bonded length was also presented and discussed. The experimental tests have shown that the NSM technique has a better performance compared to the EBR technique, independently of the installation of mechanical anchorage devices. In the case of the EBR technique, the strains in the CFRP laminate increased at its vicinities due to the clamping force applied to the anchors, which affected the final strength of the interface.

With the objective of having a solar thermoelectric system, running for 24 h a day along the different seasons of the year it is necessary to dimension the adequate storage and back-up systems. The choice of the back-up source of energy depends on how sustainable the power plant should be. In this study, the choice was the use of biomass in order to have a 100{%} renewable power plant. The selected site was the Alentejo region (Portugal). The local Direct Normal Irradiation (DNI) data was used to simulate with the System Advisor Model program (SAM) considering a solar system with north field and molten salt storage. The system needs no back-up during three months in a year. The use of biomass pellets is a viable alternative because it makes the power plant 100{%} renewable and dispatchable without loss of energy due to over-dimension of the expensive solar field and molten storage system.

This paper proposes a Tunnel FET (TFET)-based power management circuit (PMC) for ultra-low power RF energy harvesting applications. In contrast with conventional thermionic devices, the band-to-band tunneling mechanism of TFETs allows a better switching performance at sub-0.2 V operation. As a result, improved efficiencies in RF-powered circuits are achieved, thanks to increased rectification performance at low power levels and to the reduced energy required for a proper PMC operation. It is shown by simulations that heterojunction TFET devices designed with III-V materials can improve the rectification process at received power levels below-20 dBm (915 MHz) when compared to the application of homojunction III-V TFETs and Si FinFETs. For an available power of-25 dBm, the proposed converter is able to deliver 1.1 $μ }\text{W}$ of average power (with 0.5 V) to the output load with a boost efficiency of 86{%}.

The Late Jurassic Lourinhã Formation is known for its abundant remains of dinosaurs, crocodylomorphs and other vertebrates. Among this record are nine localities that have produced either dinosaur embryos, eggs or eggshell fragments. Herein, we describe and identify the first crocodiloid morphotype eggs and eggshells from the Lourinhã Formation, from five occurrences. One clutch from Cambelas, composed of 13 eggs, eggshell fragments from Casal da Rola and Peralta, one crushed egg and eggshells from Paimogo North, and four crushed eggs as well as eggshell fragments from Paimogo South. We observed and confirmed diagnostic morphological characters for crocodiloid eggshells and which are consistent with a crocodylomorph affinity, such as the ellipsoidal shape, wedge-shaped shell units, triangular extinction under cross-polarized light, and tabular ultrastructure. This material is distinctive enough to propose two new ootaxa within the oofamily Krokolithidae, Suchoolithus portucalensis, oogen. and oosp. nov., for the material from Cambelas, the most complete clutch known for crocodiloid eggs, and Krokolithes dinophilus, oosp. nov., for the remaining material. These are the oldest crocodylomorph eggs known, extending the fossil record for this group to the Late Jurassic. Furthermore, except for the clutch from Cambelas, the material was found with theropod eggs and nests, in the other four occurrences, which seem to suggest some form of biological relationship, still unclear at this point.

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