Polysaccharides in plant cell walls serve as a rich carbon and energy source, yet their structural complexity presents a barrier to efficient degradation. To address this, anaerobic microorganisms like R. flavefaciens have developed sophisticated multi-enzyme complexes known as cellulosomes, which enable the efficient breakdown of these recalcitrant polysaccharides. These complexes are assembled through high-affinity interactions between cohesin (Coh) modules in scaffoldin proteins and dockerin (Doc) modules in cellulosomal enzymes. R. flavefaciens FD-1 harbors one of the most intricate cellulosomes described to date, comprising over 200 Doc-containing proteins encoded in its genome. Despite substantial research on this cellulosome, the role of a group of truncated but functional dockerins, known as group-2 Docs, remains unclear. In this study, we present a detailed structural and binding analysis of a Coh-Doc complex involving the cohesin from the cell-anchoring scaffoldin ScaE and a group-2 Doc that bears only one of the two Ca+2-coordinating loops that characterise the canonical Docs. Our findings reveal a novel tripartite binding mechanism, in which the cohesin can simultaneously bind two distinct dockerin units in three alternative conformations. This discovery provides new insights into the modular versatility of the R. flavefaciens cellulosome and sheds light on the mechanisms that enhance its efficiency in polysaccharide degradation.

This study aims to discuss similarities and differences between knowledge models focusing on technology, developing the Global Model. This is not a new model, but rather a contribution to the articulation between different conceptual frameworks.

Este estudo visa discutir semelhanças e diferenças entre modelos de conhecimento com foco na tecnologia, desenvolvendo a partir destes o Modelo Global. Este não é um novo modelo, mas antes um contributo para a articulação entre diferentes quadros conceptuais.

Turiasauria is a clade of basal sauropod dinosaurs hitherto only known from the Middle Jurassic (Bathonian) to the Lower Cretaceous (Valanginian). A new find of a shed tooth crown from the Lower Jurassic (Pliensbachian), Halse Formation of Bornholm, Denmark, is spoon-like, asymmetrical, and heart-shaped, which identifies the tooth as turiasaurian, pushing the origin of the Turiasauria some 17 My back into the Lower Jurassic. This suggests a North Pangean/Laurasian origin of the turiasaurian clade, which then, during the Middle to Late Jurassic, dispersed through Europe, India, and Africa, with their latest representatives found in the Early Cretaceous of England and North America. Furthermore, this is the first record of a sauropod from the Pliensbachian in Europe.

Este capítulo pretende contribuir para a análise das principais fontes de ansiedade social causada pela crescente automação em Portugal. O capítulo foi baseado na revisão de literatura internacional e nacional, bem como na análise secundária de dados. Para além disso, durante o projeto DEEP VIEW foram realizadas duas entrevistas não estruturadas a especialistas em relações laborais e 13 entrevistas semiestruturadas a representantes do mundo sindical e de entidades patronais (federações e confederações), em setores particularmente relevantes para as questões associadas ao risco tecnológico.

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This paper investigates the implementation of the Extended-Matsuoka–Nakai yield criterion on a strict Limit Analysis finite element formulation. The current approach is based on a three-field mixed finite element model and the Alternating Direction Method of Multipliers optimization algorithm. With the support of duality principles two variants are derived, the lower bound and the upper bound element. The main novelty of this work is the development of an efficient iterative predictor–corrector scheme, customized for the Extended-Matsuoka–Nakai. This scheme is an indispensable requirement for this formulation. To conclude four numerical examples are presented to assess the effectiveness and efficiency of the numerical tool.

The taxonomy, chronology and dispersal patterns of hippopotamuses in Western Europe are still a matter of debate, despite the long history of researchers on these topics. In this framework, the Portuguese material has never been investigated or recently revised, and it can contribute to increasing our comprehension about Quaternary hippopotamuses. The material considered in this work, collected from sites dated between 1 Ma and 0.13 Ma, is compared with fossil Pleistocene hippopotamuses from the Iberian Peninsula, Italy, Germany and Greece, in order to contribute into the discussion on evolutionary and dispersal patterns of these large semi-aquatic mammals in Europe during the Pleistocene. Portugal deposits recorded the presence of H. antiquus, starting from 1 Ma up to 0.4 Ma, and H. cf. amphibius during the late Middle Pleistocene. In particular, the occurrence of H. antiquus in Condeixa, dated around 0.4 Ma, can be considered as the LAD of this species in Europe. The paleobiogeography and dispersal patterns of European hippopotamuses are further discussed.

ABSTRACT: Type specimens (holotypes, neotypes, syntypes, etc.) are of crucial importance because they are the only tangible evidence of the nomenclatural act that supports the understanding of paleobiodiversity. The list of the vertebrate species whose type specimen is based on fossils from Portugal is presented here. We counted 206 species, of which there are 45 bony fishes, 39 Late Jurassic Mammaliamorpha, 33 Cenozoic mammals, 28 non-avian dinosaurs, 25 non-dinosaur reptiles, 11 Cenozoic Aves, 22 Chondrichthyes and three other vertebrates. Except for the Quaternary fossil record, the type specimens can be used as a shortcut for measuring the fossil record and paleobiodiversity through geological time and rock units because they correlate in 95% with the fossil record by epoch in the case-study of fossil vertebrates of Portugal.

The paper describes the three-dimensional numerical implementation of the Lade-Duncan criterion in a finite element limit analysis (FELA) code. Validation is done using examples with a known solution. To conclude the proposed numerical tool is applied to the calculation of the ultimate bearing capacity of square footing.

Findings of Iron Age metallurgical activities related to tin metal and mining are very rare. In the present work, we present a detailed study of the Outeiro de Baltar hillfort, dated to the Late Iron Age/Early Roman period, located in a place where 20th century tin mining work took place. Elemental and microstructural analysis by portable, micro and wavelength dispersive X-ray fluorescence spectrometry (pXRF, micro-XRF and WDXRF) and scanning electron microscopy with energy dispersion spectrometer (SEM-EDS) showed that metallurgical debris found at the archaeological site is related to tin smelting and binary and ternary bronze productions. Analysis of the artefacts of diverse typologies found at the site showed that a variety of metals and alloys were in circulation and use. Samples of tin ores (cassiterite) from the region were analyzed for comparison with an archaeological tin slag from the site. The analytical results point to the production of tin metal using local cassiterite and the production of bronze by directly adding cassiterite into a smelting process. Furthermore, data of remote sensing (airborne Light Detection and Ranging (LiDAR) and historical aerial imagery) and Geographical Information System (GIS) mapping were combined with archival mining documentation and maps to retrieve a landscape context for the site. The study showed that the place of the Outeiro de Baltar hillfort (NW Iberia) was mined periodically over time.

Teacher education is central to promote the development of the professional knowledge of teachers, and
to help them achieve an appropriate integration of digital technologies, an issue that has proved to be a
difficult one. Several authors refer difficulties in the integration of the technology, emphasizing the central
role played by the teachers’ knowledge in classroom use. In this paper we discuss three models (TPACK
– Technological Pedagogical and Content Knowledge, KTMT – Knowledge for Teaching Mathematics with
Technology, PTK / MPTK - Mathematical Pedagogical Technology Knowledge), intending to identify the
main contributions of each model to a deeper understanding of how to promote the teachers’ integration
of technology in the teaching of Mathematics. The study is based on a literature review and on an analysis
of the similarities and differences among the models and its use. On this analysis we identify common
influences among the models as well as influences from other research areas. The main conclusions
achieved point to a common base to all the models considered, but also to several differences among
them, being that some of the models emphasize the role of technology and its impact on Mathematics
learning, but others go further, intending to integrate in the model elements based on the research on
technology or even other theories such as the one on instrumental genesis.

Due to the innovative possibilities of digital technologies, the issue of increasing automation is once again on the agenda – and not only in the industry, but also in other branches and sectors of contemporary societies. Although public and scientific discussions about automation seem to raise relevant questions of the “old” debate, such as the replacement of human labor by introducing new technologies, the authors focus here on the new contextual quality of these questions. The debate should rethink the relationship between technology and work with regard to quantitative and qualitative changes in work. In this article, our example will be the introduction of automation in industry, which has been reflected in the widely recognized study by Frey and Osborne in 2013. They estimated the expected impacts of future computerization on US labor market outcomes as very high, specifically regarding the number of jobs at risk. Surprisingly, this study was the starting point of an intensive international debate on the impact of technologies on the future of work and the role of technological change in working environments. Thus, according to the authors, “old” questions remain important, but they should be reinterpreted for “new” societal demands and expectations of future models of work.

This paper intended to provide a vision on the potential consequences of the introduction of emerging air transportation system which was based on a section of the thesis to contribute to the forming of public and policy opinion.Especially this paper tried to understand whether there were enough positive social and environmental impacts in terms of potential benefits to continue the efforts.The negative impacts are beyond the scope of this paper. Limitations of the current ground and airline transportation systems, increasing congestion, poor block speed, combined with expanding population and demand for affordable on-demand mobility are driving the development of future transportation technology and policy. The third wave of aeronautic,e VTOL (Vertical Takeoff and landing) Air Transportation System, is envisioned as the next logical step in the natural progression and could bring about great new capabilities for society that would bring aviation into a new age of being relevant in daily lives. Considering door to door block time, e VTOL Air Transportation System has the potential to achieve anotherfive-fold increase over the auto today as the auto provided ten-fold increase over the horse, and a daily mobility reach of 125 to 250 miles.The main benefits will be on-demand, point-to-point,safe travel, further and anywhere in less travel time with almost zero environmental impact for general people. Besides, a successful implementation and sustainable transition will depend on overcoming technological hurdles, regulatory frameworks, operational safety, cost competitiveness, and sensibilities of the affected communities.