Xylella fastidiosa is a xylem-limited, Gram-negative phytopathogenic bacterium responsible for severe diseases affecting a wide range of economically important crops worldwide. Its recent expansion into Europe has reinforced its status as a major quarantine pathogen. However, research on its physiology and antimicrobial susceptibility remains limited by its slow and fastidious growth under laboratory conditions, which typically requires 7–30 days for detectable development. We report a new laboratory-adapted culture medium, XFG; that supports robust growth of X. fastidiosa strain DSM 10026/PYCC 9740 in both liquid and solid formulations compatible with established physiological and antimicrobial testing protocols. In liquid culture, bacterial populations reached the stationary phase within three days, while visible and isolated colonies formed on solid medium within the same time frame. Growth kinetics were confirmed by optical density measurements, demonstrating a shortened lag phase and reproducible exponential growth. Proof-of-concept antimicrobial susceptibility testing revealed high sensitivity to rifampicin and tetracycline (MIC = 0.25 μg/mL), and to chloramphenicol and ampicillin (MIC = 0.99 μg/mL). These MIC values are consistent with those previously reported using conventional media, supporting the robustness and applicability of the proposed system. Overall, these results establish XFG as a system for reproducible X. fastidiosa growth under laboratory conditions, enabling high-throughput phenotyping of this important plant pathogen.

The integration of digital technology to promote mathematical learning remains challenging for teachers. The continuous difficulty in fully achieving technology’s potential highlights the need to further examine and develop models addressing teachers’ knowledge in relation to technology. In this Research Forum, we analyse existing technology-related knowledge models, exploring similarities and differences. We also consider how these models have been used in research, highlighting tensions driven by emerging technologies, and resulting extensions of the models. Finally, we discuss possible integrations across frameworks and the potential to include dimensions beyond knowledge. This discussion aims to deepen understanding of current models and suggest directions and a research agenda for future development.

A problem that has been studied for a long time in Vegetation Science consists in finding distinct groups among a collection of vegetation samples (relevés). Recently a criterion has been proposed to capture the patterns of differential species among the groups from any arbitrary M-cluster of relevés. The criterion optimization is quite complex (NP-hard) as it implies searching a huge set of possible combinations of relevés into M groups. In this paper we give an integer linear programming formulation to optimize the criterion for M=2, that can be used to solve moderately-sized data problems; we also describe a greedy randomized adaptive search procedure and a simulated annealing algorithm for arbitrary M. We combined these different approaches and prepared a collection of software functions, which are available in the open-source R package diffval. We illustrate the use of such functions using real-world data.

The adoption of I4.0’s technologies in the work cells accompanied by societal changes calls for new approaches to manage the current production systems. Nevertheless, the current models simulating the behavior of the work cells limit the representation of the operators to the average human, without regard for their individual characteristics, cognitive abilities or psychosocial state. The aim of this paper is to achieve two objectives: firstly, the authors propose a conceptual model for enhancing a human-centered production environment. Secondly, the paper summarizes how the literature characterizes the different dimensions of relations between cognitive and organizational factors. By integrating the collaborative, psychological, social, cognitive, organizational and system performance dimensions, the proposed model focuses on the relationships between these dimensions. Thus, operational models should be closer to the real production environment to improve the design choices of the manufacturing systems.

The potential of technology to enhance the learning of mathematics is widely acknowledged. However, realizing this potential requires teachers to possess the knowledge to effectively integrate it into their teaching practices. This study aims to characterize the mathematics teacher's knowledge when using different technologies in teaching and learning. It also aims to study the existence of relationships between the specific domains of the Knowledge for Teaching Mathematics with Technology – KTMT and the integration of graphing calculator and Excel. The methodology adopted is qualitative with an interpretative approach, using a case study of Mathematics teacher in the 11th grade (16-17 years old) with extensive experience in the use of technology. This study shows that different KTMT knowledge is mobilized, according to the technology chosen and the specific characteristics of each of the technologies used influenced the teacher's pedagogical choices.

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O sítio de Silveirinha é uma das localidades de mamíferos mais conhecidas da Paleontologia do Cenozoico de Portugal e da Europa em geral. Graças à sua rica e diversificada associação de mamíferos, com mais de 30 taxa, foi posicionado no Eocénico inferior (início do Ypresiano, MP7, ca. 55,8 M.a.), sendo o local mais antigo da Europa desta Época, devido à presença única de taxa típicos do Paleocénico superior, juntamente com outras espécies já características do Eocénico inferior. Este estudo irá rever o material de pequenos mamíferos deste sítio, conservado na coleção clássica da Universidade Nova de Lisboa, a fim de fazer uma actualização taxonómica à luz das publicações mais recentes.

The emergence of the platform economy is the most recent model of outsourcing practices and the flexibilization of the organisation of production supported by the development of information and communication technologies. The spread of this business model has led to the expansion of different precarious forms of employment, the most common being the bogus self-employed. The business model based on digital platforms also has the effect of limiting the capacity of trade unions to represent and defend workers, preventing or limiting, for example, resource to collective bargaining. This article compares the trade union revitalisation strategies deployed in Spain and Portugal in the rider sector by traditional trade unions and associations of delivery workers and precarious workers. The article shows how, in both countries, unions have combined organising and mobilisation strategies with actions aimed at influencing political and regulatory expectations. However, there is evidence of a greater experimentation of practices in the Spanish case, as well as a greater impact and influence of their strategies in political terms. The article also reveals important differences between the two countries regarding the relationship between traditional trade unions and delivery workers' associations.

{The Upper Jurassic Lourinhã Formation is well known for its rich assemblage of fossil vertebrates. In this formation, ornithopod dinosaurs are represented by two iguanodontian species, Eousdryosaurus nanohallucis and Draconyx loureiroi. We recently became aware of unreported material belonging to the holotype of Draconyx loureiroi, consisting of partially articulated manual elements. We here re-describe the holotype specimen ML 357, including the newly discovered material. The specimen was subjected to CT-scanning and its surface data used to assess anatomical characters. Linear measurements of metatarsal III were used to estimate the body length of the specimen. The Draconyx loureiroi holotype was included in two datasets and analysed with maximum parsimony and Bayesian inference approaches to estimate evolutionary rates among Iguanodontia. We present evidence that Draconyx loureiroi is a valid taxon nested in Styracosterna and is clearly diagnosable by a unique combination of characters. Both maximum parsimony and Bayesian inference indicate high evolutionary rates across the Jurassic/Cretaceous transition for the base of Iguanodontia. Length estimation suggests that Draconyx loureiroi was a relatively small, bipedal and possibly cursorial animal. Given its basal phyletic position, we interpret this bauplan was the ancestral condition for Styracosterna, that only later in the Cretaceous evolved into giant quadrupedal forms.}

In this paper we compute the rank and exhibit a presentation for the monoids
of all $P$-stable and $P$-order preserving partial permutations on a finite set
$\Omega$, with $P$ an ordered uniform partition of $\Omega$. These (inverse)
semigroups constitute a natural class of generators of the pseudovariety of
inverse semigroups ${\sf NO}$ of all normally ordered (finite) inverse
semigroups.