The high global burden of over one million annual lethal fungal infections reflects a lack of protective vaccines, late diagnosis and inadequate chemotherapy. Here, we have generated a unique set of fully human anti-Candida monoclonal antibodies (mAbs) with diagnostic and therapeutic potential by expressing recombinant antibodies from genes cloned from the B cells of patients suffering from candidiasis. Single class switched memory B cells isolated from donors serum-positive for anti-Candida IgG were differentiated in vitro and screened against recombinant Candida albicans Hyr1 cell wall protein and whole fungal cell wall preparations. Antibody genes from Candida-reactive B cell cultures were cloned and expressed in Expi293F human embryonic kidney cells to generate a panel of human recombinant anti-Candida mAbs that demonstrate morphology-specific, high avidity binding to the cell wall. The species-specific and pan-Candida mAbs generated through this technology display favourable properties for diagnostics, strong opsono-phagocytic activity of macrophages in vitro, and protection in a murine model of disseminated candidiasis.

Semiconductor nanowires are mostly processed by complex, expensive and high temperature methods. In this work, with the intent of developing zinc tin oxide nanowires (ZTO NWs) by low-cost and low-complexity processes, we show a detailed study on the influence of chemical parameters in the hydrothermal synthesis of ZTO nanostructures at temperatures of only 200 °C. Two different zinc precursors, the ratio between zinc and tin precursors, the concentration of the surfactant agent and of the mineralizer were studied. The type and the crystallinity of the nanostructures was found to be highly dependent on the used precursors and on the concentration of each reagent. Conditions for obtaining different ZTO nanostructures were achieved, namely Zn2SnO4 nanoparticles and ZnSnO3 nanowires with length ≈ 600 nm, with the latter being reported for the first time ever by hydrothermal methods without the use of seed layers. Optical and electrical properties were analyzed, being obtained band gaps of 3.60 and 3.46 eV, fo...

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This paper presents a wireless energy transfer system operating at the frequency values of kHz order: modeling, simulation, and comparison with prototype measurement results. Wireless energy transfer system model using finite element method was carried out to simulate the electric field and the magnetic flux density for different air gap sizes between the transmitter and the receiver coils. Results are presented and compared with the electromagnetic emission measurements radiated by the wireless energy transfer system prototype. The electric field comparison between the simulated and the prototype measurement values shows an error of roughly 8.7{%}. In the recent years, the interest in the wireless energy transfer technology, especially for electric vehicles batteries charging, is rapidly increasing. As a result of the increasing application of this technology in the industrial and consumer electronic products, more concerns are raised about the electromagnetic compatibility, since the wireless energy transfer systems produce electromagnetic emissions in the surrounding environment.

This paper presents a wireless energy transfer (WET) system operating at the frequency of tens of kHz. It treats the modeling and simulation of WET prototype and its comparison with experimental measuring results. The wireless energy transfer system model was created to simulate the electric field between the emitting and the receiving coils, applying the finite element method. The results from the simulation are compared to the measured values of the electric field emission from the wireless energy transfer equipment. In the recent years the interest in the WET technology, especially for the electric vehicles (EV) batteries charging, is rapidly growing. The WET systems pollute the environment by electromagnetic emissions. Due to the expanding use of this technology in industrial and consumer electronics products, the problems associated with the electromagnetic compatibility (EMC), and the adverse impact on the human health becomes highly important.

This paper presents the experimental results obtained in the wireless energy transfer (WET) system prototype based on coils: circular or planar. With these experimental results we can choose the tuning settings to improve the efficiency of power transmission of the WET systems. In WET for electric vehicle batteries charging, the coil shape and the range between the coils are the most important issues of those systems.

This paper presents a study of the behaviour and load capacity of Steel Fibre Reinforced Concrete (SFRC) flat slabs under monotonically increased concentrated vertical loads. The SFRC was used only in the local region of the slab-column connection, as the rest of the slab was cast using normal concrete (NC) without fibres. The six experimental test specimens had a thickness of 150 mm with different longitudinal reinforcement ratios, using a non-uniform distribution over the slab width. The concretes used were made with different Dramix® 4D 65/60 BG steel fibre contents (0%, 0.5 %, 0.75% and 1.0% volume content). The slab tests were complemented by flexural tests on notched-beams. This made it possible to determine the tension behaviour of the different concretes used, through a linear post-cracking behavior and inverse analysis. The inverse analysis made it possible to define the stress-crack opening relationship that characterize the tension behaviour of SFRC and to relate it to the observed behaviour and load capacity of the tested slabs. The tests results show that the tensile behaviour of the SFRC plays an important role in the behavioural and load capacity of the slabs and that it can be considered relevant to physically based models.

This paper presents an experimental study of four flat slab specimens subjected to combined vertical and horizontal cyclic loading. Steel fibre-reinforced concrete (SFRC) was used only in the local region of the slab–column connection, while the rest of the slabs were cast using normal concrete. The specimens measured 4·15 m × 1·85 m × 0·15 m and were connected to two steel half columns by 0·25 m × 0·25 m rigid steel plates, prestressed against the slab using steel bolts, to ensure monolithic behaviour. The specimens were tested using an innovative test setup system that accounted for important factors, such as the ability of bending moment redistribution, line of inflection mobility and assured equal vertical displacements at the opposite slab borders, and symmetrical shear forces. Results show that the presence of SFRC in the slab–column connection region is effective in increasing the deformation capacity of slab–column connections, allowing the increase of horizontal drift ratios.