A procura de soluções de reforço mais duráveis e de fácil aplicação tem levado à utilização crescente dos compósitos de FRP (Fiber Reinforced Polymer) no reforço de estruturas, dada a sua resistência à corrosão, o baixo quociente peso/resistência mecânica, a sua moldabilidade, a facilidade de aplicação e a eliminação de estruturas de suporte. No reforço estrutural de vigas de betão armado com compósitos de FRP, são tradicionalmente utilizados dois tipos de técnicas: os sistemas em que o laminado é colado pelo exterior (EBR - Externally-Bonded Reinforcement) ou aqueles em que o laminado é inserido em rasgos previamente abertos na camada de recobrimento (NSM - Near Surface Mounted). No entanto, as técnicas utilizadas, o comportamento elástico-linear destes materiais e as roturas tendencialmente frágeis das soluções condicionam a sua utilização em estruturas onde se pretende alguma ductilidade. A técnica de reforço NSM apresenta algumas vantagens em relação à técnica EBR, nomeadamente ao nível da proteção das armaduras [1]. Além disso, o desempenho em termos de ductilidade do sistema e resistência final excede a técnica EBR. Contudo, diversos ensaios experimentais [2-5] têm mostrado que roturas prematuras [6] da ligação na interface ou o destacamento do betão na zona do recobrimento entre a face inferior das armaduras ordinárias e as armaduras de reforço podem limitar significativamente a eficiência do sistema, originando modos de rotura frágeis e desperdício de material por falta de otimização da quantidade de material aplicado [1]. A fim de evitar a rotura prematura das soluções de reforço tradicionais (EBR e NSM), foi concebido na Universidade NOVA um sistema inovador de reforço intitulado CREatE (Continuous Reinforcement Embedded at Ends). O sistema CREatE foi idealizado para ser utilizado com diversos materiais [1, 5] e diferentes elementos estruturais, tais como vigas [1, 3], pilares [7], pavimentos [8], lajes ou paredes, em que é necessário aumentar a sua capacidade resistente através de armaduras pós-instaladas. A solução de reforço CREatE caracteriza-se pela utilização de armaduras contínuas embutidas nas extremidades do elemento estrutural sem o uso de dispositivos mecânicos para as fixar. Antes da ancoragem da armadura de reforço no interior do elemento, é necessário utilizar uma curva de transição suave para al terar a forma da armadura de reforço e evitar a concentração de tensões no la minado de CFRP (Carbon Fiber Reinforced Polymer) ou na interface e, desta forma, ter um fluxo gradual de tensões transmitidas à zona de ancoragem existente no interior do elemento. Para validar a solução CREatE foi realizada uma campanha de ensaios à flexão de vigas de betão armado com seção em T, uma altura total de 0,3m, um vão livre de 3,0m e reforçadas com laminados de CFRP recorrendo a diferentes técnicas (EBR, NSM e CREatE). As vigas foram testadas à flexão em 4 pontos, tendo-se obtido resultados promissores (Figura 1), com a eliminação na técnica CREatE dos modos de rotura prematuros. Na Figura 2 é possível observar uma viga ensaiada com a técnica CREatE em que se detetam aberturas de fendas significativas sem que se verifique qualquer rotura prematura do sistema. Além da eliminação dos modos de rotura prematuros, os ensaios comprovam que a técnica CREatE permite o incremento da ductilidade (Figura 1) e a exploração total da capacidade do CFRP [1, 3, 5].

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Spectrochimica Acta Part B: Atomic Spectroscopy, 130 (2017) 35-38. doi:10.1016/j.sab.2017.02.006

Deconstruction of cellulose, the most abundant plant cell wall polysaccharide, requires the cooperative activity of a large repertoire of microbial enzymes. Modular cellulases contain non-catalytic type A Carbohydrate-Binding Modules (CBMs) that specifically bind to the crystalline regions of cellulose, thus promoting enzyme efficacy through proximity and targeting effects. Although type A CBMs play a critical role in cellulose recycling, their mechanism of action remains poorly understood. Here we produced a library of recombinant CBMs representative of the known diversity of type A modules. The binding properties of 40 CBMs, in fusion with an N-terminal green fluorescence protein (GFP) domain, revealed that type A CBMs possess the ability to recognize different crystalline forms of cellulose and chitin over a wide range of temperatures, pHs and ionic strengths. A Spirochaeta thermophila CBM64, in particular, displayed plasticity in its capacity to bind both crystalline and soluble carbohydrates under a wide range of extreme conditions. The structure of S. thermophila StCBM64C revealed an untwisted, flat, carbohydrate-binding interface comprising the side chains of four tryptophan residues in a coplanar linear arrangement. Significantly, two highly conserved asparagine side chains, each one located between two tryptophan residues, are critical to insoluble and soluble glucan recognition but not to bind xyloglucan. Thus, CBM64 compact structure and its extended and versatile ligand interacting platform illustrates how type A CBMs target their appended plant cell wall degrading enzymes to a diversity of recalcitrant carbohydrates under a wide range of environmental conditions.

Compressive stresses created by lateral external pressure on laminates are an important factor on success of the use of mechanical anchorage of externally bonded reinforcement (EBR). A program of double shear tests with imposed normal stresses on GFRP plates bonded to a concrete surface and a bond-slip model are described. Results generated numerically are summarized and used as reference values against those obtained after accelerated aging by freeze-thaw cycles, and temperature cycles of the same amplitude but range closer to the glass vitreous temperature. Numerical modelling showed that the bonded length is fully stressed prior to failure. Increasing lateral pressure led to a larger maximum bond stress and strength at the interface. Cohesion, fracture energy and internal friction angle changes are calculated and used to analyze the effects of the aforementioned cycles on the expected behaviour of the GFRP-concrete joints, namely at the interface.

A utilização de Polímeros Reforçados com Fibras (FRP) no reforço de estruturas de Betão Armado (BA) tem tido cada vez mais aceitação devido à sua elevada resistência e rigidez, baixo peso específico e excelente resistência aos efeitos dos agentes ambientais. No entanto, actualmente, é comum utilizarem-se técnicas de reforço que dificilmente permitem tirar partido da resistência total destes materiais. Com o objectivo de explorar a capacidade total de Polímeros Reforçados com Fibras de Carbono (CFRP), foram estudadas e desenvolvidas duas novas técnicas de reforço de vigas à flexão designadas por Continuous Reinforcement Embedded at Ends (CREatE) e Horizontal Near Surface Mounted Reinforcement (HNSMR). Posteriormente realizou-se um estudo comparativo entre o desempenho destes sistemas de reforço e o de duas outras técnicas já estudadas e usuais, nomeadamente os sistemas Externally Bonded Reinforcement (EBR) e Near Surface Mounted Reinforcement (NSMR). A técnica CREatE provou ser a mais eficaz de todas as alternativas testadas mobilizando a totalidade do compósito de CFRP e dotando as vigas de BA com uma maior capacidade resistente e com uma ductilidade mais elevada.Como complemento deste trabalho experimental, desenvolveu-se também um programa de cálculo em MATLAB, capaz de simular o problema em estudo através de um modelo numérico de análise não linear através do equilíbrio de secções. A representatividade dos dados obtidos foi verificada através de uma análise comparativa entre os valores numéricos e os obtidos experimentalmente.The use of Fiber Reinforced Polymers (FRP) in order to strengthen Reinforced Concrete (RC) structures has been increasingly accepted due to their strength and stiffness, low weight and excellent resistance to the effects of environmental aggressive agents. However, the bonding techniques available and described in the literature can not allow the full use of the mechanical properties of these materials and premature failures are often observed and described by several researchers. In order to explore the full capacity of CFRP composites, two new bonding strengthening techniques of RC beams when subjected to 4-point bending tests were studied and developed. For these new techniques, the designation of Continuous Reinforcement Embedded at Ends (CREatE) and Horizontal Near Surface Mounted Reinforcement (HNSMR) has been assigned. Posteriorly, a comparative study has been carried out between those strengthening systems performance and two traditional techniques, namely, the Externally Bonded Reinforcement (EBR) and Near Surface Mounted Reinforcement (NSMR). The CREatE technique has proved to be the most effective of all alternatives tested, with the full utilization of the CFRP composite and the highest strength, combined with the highest ductility. A code using MATLAB software was developed as a complement of this experimental work, which is able to simulate the problem under study through a nonlinear numerical model based on the equilibrium of sections. The representativeness of the numerical data has been verified afterwards through a comparative analysis between those and the experimental results.

In this paper, we study the performance of a portable energy dispersive X‐ray fluorescence spectrometer by making use of different filter configurations at the X‐ray tube output. To fulfill this purpose,...

We show that a physical trigger, a non-ionizing infrared (IR) radiation at wavelengths strongly absorbed by liquid water, can be used to induce and kinetically control protein (periodic) self-assembly in solution. This phenomenon is explained by considering the effect of IR light on the structuring of protein interfacial water. Our results indicate that the IR radiation can promote enhanced mutual correlations of water molecules in the protein hydration shell. We report on the radiation-induced increase in both the strength and cooperativeness of H-bonds. The presence of a structured dipolar hydration layer can lead to attractive interactions between like-charged biomacromolecules in solution (and crystal nucleation events). Furthermore, our study suggests that enveloping the protein within a layer of structured solvent (an effect enhanced by IR light) can prevent the protein non-specific aggregation favoring periodic self-assembly. Recognizing the ability to affect protein-water interactions by means of IR radiation may have important implications for biological and bio-inspired systems.

Spectrochimica Acta Part B: Atomic Spectroscopy, 129 (2016) 1-7. doi:10.1016/j.sab.2016.12.006

Spectrochimica Acta Part B: Atomic Spectroscopy, 130 (2017) 60-66. doi:10.1016/j.sab.2017.02.012

Piezoelectric energy harvesting has received great attention over the last 20 years. The main goal of this work is to discuss the potential advantages of introducing non-linearities in the dynamics of a beam type piezoelectric vibration energy harvester. The described device is essentially a cantilever beam partially covered by piezoelectric material with a force electromagnetically applied to the beam. Through experimental tests it has been confirmed the benefits of introducing non-linearities in these types of systems.

This paper investigates whether the model uncertainty of reinforced recycled aggregate concrete (RAC) beams subjected to bending differs from that of reinforced natural aggregate concrete (NAC) beams. An introductory remark concerning the importance of the codification of RAC structural design is made and notions concerning model uncertainties and their role on structural codification are given. Afterwards, the criteria used in the construction of a database of RAC and NAC beams are referred before presenting the key findings of an analysis on the model uncertainty of the cracking, yielding and ultimate moments of beams subjected to four-point bending tests. The analytical moments were calculated following Eurocode 2 provisions. Probabilistic models for model uncertainties are proposed. Negligible differences in the model uncertainty of NAC and RAC beams are reported.

Cellulosomes are sophisticated multi-enzymatic nanomachines produced by anaerobes to effectively deconstruct plant structural carbohydrates. Cellulosome assembly involves the binding of enzyme-borne dockerins (Doc) to repeated cohesin (Coh) modules located in a non-catalytic scaffoldin. Docs appended to cellulosomal enzymes generally present two similar Coh-binding interfaces supporting a dual-binding mode, which may confer increased positional adjustment of the different complex components. Ruminococcus flavefaciens’ cellulosome is assembled from a repertoire of 223 Doc-containing proteins classified into 6 groups. Recent studies revealed that Docs of groups 3 and 6 are recruited to the cellulosome via a single-binding mode mechanism with an adaptor scaffoldin. To investigate the extent to which the single-binding mode contributes to the assembly of R. flavefaciens cellulosome, the structures of two group 1 Docs bound to Cohs of primary (ScaA) and adaptor (ScaB) scaffoldins were solved. The data revealed that group 1 Docs display a conserved mechanism of Coh recognition involving a single-binding mode. Therefore, in contrast to all cellulosomes described to date, the assembly of R. flavefaciens cellulosome involves single but not dual-binding mode Docs. Thus, this work reveals a novel mechanism of cellulosome assembly and challenges the ubiquitous implication of the dual-binding mode in the acquisition of cellulosome flexibility.