We report the 94 % assignment of DVU2108, a protein belonging to the Orange Protein family, that in Desulfovibrio vulgaris Hildenborough forms a protein complex named the Orange Protein complex. This complex has been shown to be implicated in the cell division of this organism. DVU2108 is a conserved protein in anaerobic microorganisms and in Desulfovibrio gigas the homologous protein was isolated with a novel Mo-Cu cluster non-covalently attached to the polypeptide chain. However, the heterologously produced DVU2108 did not contain any bound metal. These assignments provide the means to characterize the interaction of DVU2108 with the proteins that form the Orange Protein complex using NMR methods.

Abstract The present work shows the effect of magnesium doping on structural, optoelectrical and electrical properties of Cu2O thin films prepared by spray pyrolysis. The variation in the concentration of Mg shows significant impact on the final thin film properties, whereas the film doped with 0.5 at{%} of Mg exhibited major property improvements in comparison with the undoped thin film and among the other concentrations tested. This condition was further applied for the deposition of an absorber layer in a heterojunction solar cell array with a gradient in thicknesses of active layers to investigate the impact of changing thicknesses on the PV parameters of the solar cell. TiO2 was used as a window layer and the 0.5 at{%} Cu2O doped film as an absorber layer. The produced heterojunction solar cell array was further exposed to a rapid thermal annealing treatment. The I–V measurements show an open circuit voltage of up to 365 mV and a short circuit current density, which is dependent on absorber layer thickness, and reaches to a maximum value of 0.9 mA/cm2.

Portable X-ray fluorescence spectrometers (p-XRF) have been used in many fields of application/studies like art, archaeology, heavy metals in soil, rocks and ores characterization, and have been a powerful tool for a rapid non-destructive in-situ analysis, without any sample preparation required. This approach was applied in the present case, to distinguish the origin of the fossil bones of two dinosaur specimens from different localities that were accidentally put together in the museum a few years ago. Fossil bones with sedimentary matrix associated were stored together until today in the collection of Geological Museum (Lisbon) and regarded as one single specimen. One set of bones is part of the holotype MG 5787 of the ankylosaur Dracopelta zbyszweskii, which was discovered at Praia do Sul, and described in 1980, while the other, is an undescribed half skeleton of dacentrurine stegosaur, unearthed in the 1960’s at Atouguia da Baleia, near Peniche (both in the coast of central Portugal, distanced about 100 km from each other). Since both specimens are highly valuable for paleontology, a study was developed with the aim of separating and reconstituting the two specimens. The handheld p-XRF (Genius 9000+7000 from Skyray Instrument) was directly used in the sedimentary matrix when it was separated from the bone, and the measure of the chemical content was performed in the intermediate layer between the surface and the bone, to avoid contaminations. Although the light elements could not be attained, because the analyzer is not equipped with the option of gas charging system, the spectra obtained showed differences mainly in the ratio K/Ca, allowing distinguishing the provenance of the bones (Atouguia or Praia do Sul). These results were compared with chemical analysis obtained with XRF laboratorial equipment and complemented by the mineralogical study through X-ray diffraction (XRD) of the sediments where the bones fossilized. The difference observed in the mineralogical constitution of the sedimentary matrix from the two localities (mainly quartz, calcite, feldspars and micas with variable content) explains the variation in the values found for the ratio K/Ca (<0.5 for Atouguia and >>1 for Praia do Sul). The data obtained will be presented and discussed focusing on the importance of using a portable X-ray fluorescence analyzer applied to the reconstitution of dinosaur fossils that proved to be very useful in the present case.

The use of Fiber Reinforced Polymers (FRP) in the strengthening of timber structures is quite recent and few studies have discussed the debonding between these materials. The analysis of the Mode II debonding process between FRP composites and timber elements may be of great importance because this mode is predominant in the case, for instance, of the bending of beams. Knowing the appropriate bond-slip model to use on the estimation of the performance of FRP-to-timber interfaces is greatly relevant. Under such circumstances, a detailed knowledge of all the states that CFRP-to-timber interfaces are subjected to is important as well. The current work gives answers to these aspects proposing an analytical solution based on a tri-linear bond-slip model that is capable of describing precisely the full-range debonding behavior of FRP-to-timber interfaces. Thus, the purpose of this study is to contribute to existing knowledge with an analytical solution capable of describing the full-range debonding process between a FRP composite and a substrate. The analytical solutions herein proposed are also compared with the results obtained from several experiments based on single-lap shear tests. Comparisons at different load levels and different bonded lengths are presented. The slips, strains in the CFRP composite and bond stress distributions within the bonded interface are emphasized in the text. The complete load-slip response of CFRP-to-timber interface is also analyzed. Each state of the debonding process is described and each one is identified in the load-slip curve.

The usability of requirements engineering (RE) techniques has been recognised as a key factor for their successful adoption by industry. RE techniques must be accessible to stakeholders with different backgrounds, so they can be empowered to effectively and efficiently contribute to building successful systems. When selecting an appropriate requirements engineering technique for a given context, one should consider the usability supported by each of the candidate techniques. The first step towards achieving this goal is to gather the best evidence available on the usability of RE approaches by performing a systematic literature review, to answer one research question: How is the usability of requirements engineering techniques and tools addressed? We systematically review articles published in the Requirements Engineering Journal, one of the main sources for mature work in RE, to motivate a research roadmap to make RE approaches more accessible to stakeholders with different backgrounds.

O reforço das estruturas de betão armado com compósitos de FRP tem tido um incremento considerável nos últimos anos devido às características de durabilidade e das elevadas relações resistência-peso e rigidez-peso dos compósitos de FRP comparativamente com outros materiais de reforço. A fim de analisar o comportamento de diferentes soluções de reforço estrutural de vigas de betão armado (BA) com compósitos de FRP foi realizado um programa experimental que incluiu o ensaio de vigas de BA reforçadas com as seguintes técnicas de reforço: EBR (Externally-Bonded Reinforcement), NSM (Near Surface Mounted) e pela técnica CREatE (continuous reinforcement embedded at ends). As vigas de BA ensaiadas tinham seção em T, com um vão de 3,0 m e uma altura de 0,3 m e foram solicitadas em flexão em 4 pontos e testadas até a rotura. A técnica CREatE provou ser a mais eficaz das três alternativas testadas mobilizando a totalidade do CFRP e apresentando a maior capacidade resistente e a ductilidade mais elevada.

A procura de soluções de reforço mais eficientes que permitam aumentar a capacidade resistente de elementos estruturais sujeitos a flexão levou ao desenvolvimento de um sistema inovador de aplicação de armaduras de reforço coladas pelo exterior. Neste artigo descrevem-se os ensaios experimentais realizados e analisam-se os resultados obtidos com vigas de betão armado reforçadas com armaduras pós-instaladas de aço inoxidável com diferentes técnicas: Externally Bonded Reinforcement (EBR), Near Surface Mounted (NSM) e com o novo sistema de reforço desenvolvido - Continuous Reinforcement Embedded at Ends (CREatE). As vigas ensaiadas monotonicamente até à rotura em flexão de quatro pontos têm seção transversal em T e um vão livre de três metros. No novo sistema de reforço as armaduras são ancoradas por aderência no interior do elemento estrutural, o que associado à utilização de armaduras em aço inoxidável, possibilita aumentos de resistência e ductilidade consideráveis face às técnicas tradicionais de colagem pelo exterior.

A utilização de materiais compósitos de matriz polimérica (Fiber Reinforced Polymers - FRP) como armadura de reforço de diferentes elementos estruturas de várias naturezas (e.g. betão armado, aço, madeira ou alvenaria) tem vindo a suscitar o interesse da comunidade científica internacional. Inicialmente, a simples colagem pelo exterior dos compósitos de FRP aos elementos estruturais permitiu identificar um fenómeno de descolamento prematuro do compósito para níveis de extensão muito aquém dos seus limites de rotura. Com base na experimentação, algumas teorias têm surgido no sentido de explicar o fenómeno do descolamento prematuro dos FRPs. Por outro lado, outras técnicas de reforço têm sido propostas com o objetivo de adiar ou simplesmente eliminar esse fenómeno. Neste trabalho, são analisadas diferentes ligações coladas entre laminados de CFRP e outros materiais tais como, o betão armado, o aço e a madeira. Duas técnicas de reforço por colagem vulgarmente citadas na literatura internacional foram utilizadas: Externally Bonded Reinforcement (EBR) e Near Surface Mounted (NSM). Os resultados experimentais permitiram constatar que o desempenho local das ligações estudadas é distinto, tendo-se observado que as relações entre a tensão de aderência e o deslocamento relativo entre superfícies é: (i) na ligação CFRP/betão do tipo não linear e caracteriza-se por, após atingir-se uma tensão de aderência máxima, o descolamento ocorre quando a tensão de aderência tende para zero; (ii) na ligação CFRP/aço o desempenho é do tipo bi-linear, i.e. com um troço inicial retilíneo até atingir-se uma tensão de pico seguindo-se um troço linear descendente até tensão de aderência nula; e (iii) na ligação CFRP/madeira o desempenho é do tipo tri-linear, i.e. similar à ligação CFRP/aço mas com um troço constante a seguir ao troço linear descendente e que se esgota para um deslocamento relativo último.

O dimensionamento de vigas de madeira aos Estados Limites de Utilização (ELUt) tem limites muito apertados tanto para ações de curto prazo como para ações de longo prazo. Uma solução eficiente para este problema passa por aumentar as seções transversais das vigas. Porém, este tipo de solução não só acarreta um aumento de custos como também altera profundamente arquitetura original do edifício abrindo, por conseguinte, uma oportunidade para encontrar outras soluções mais eficientes. Neste sentido, o uso de armaduras de reforço em vigas de madeira pode ser considerado como uma solução promissora uma vez que as estruturas, novas ou velhas, manteriam o aspeto estético original sem introduzir nos elementos reforçados, um aumento significativo do seu peso próprio, melhorando o seu desempenho face a ações de curto e longo prazo. O presente estudo é dedicado à análise de vigas de madeira antigas reforçadas à flexão com materiais compósitos de fibras de carbono, vulgarmente designados na literatura internacional por Carbon Fiber Reinforced Polymers (CFRP). Neste trabalho, foram reforçados e ensaiados à flexão pavimentos antigos de madeira tendo-se analisado os respetivos desempenhos aquando da utilização de uma técnica de reforço por colagem tradicional (Externally Bonded Reinforcement - EBR) e aquando da utilização de uma técnica de reforço por colagem inovadora (Continous Reinforcement Embedded at Ends - CREatE). Os ensaios experimentais permitiram verificar que a técnica de colagem inovadora CREatE confere aos pavimentos de madeira uma maior rigidez e resistência face à técnica tradicional conseguindo se mobilizar a totalidade do compósito de CFRP.

Energy consumption is becoming an evident concern to software developers. This is even more notorious due to the propagation of mobile devices. Such propagation of devices is also influencing software development: a software system is now developed has a set of similar products sharing common features.
In this short paper, we describe our methodology aim at static and accurately predict the energy consumption of software products in such variability systems, typically called software product lines.

O reforço à flexão de vigas de betão armado tem apresentado uma evolução com tendência para soluções onde são utilizadas armaduras à base de materiais compósitos de fibras de Carbono, Vidro, Basalto ou Aramida, aplicadas com as técnicas Externally Bonded Reinforcement (EBR) ou Near Surface Mounted (NSM). No entanto, o comportamento elástico-linear destes materiais e as roturas tendencialmente frágeis das soluções condicionam a sua utilização em estruturas onde se pretende alguma ductilidade. Por conseguinte, procurou-se desenvolver um sistema de reforço estrutural alternativo e inovador em que os materiais de reforço aplicados, conjuntamente com a solução de reforço, conseguissem minimizar ou eliminar os riscos de roturas prematuras e ao mesmo tempo aumentassem a ductilidade dos elementos reforçados. Neste trabalho, apresenta-se em pormenor este novo sistema de reforço à flexão de vigas de betão armado com armaduras de aço inoxidável ancoradas internamente por aderência. Neste sistema de reforço as armaduras são contínuas e pós-instaladas pelo exterior, ficando as extremidades ancoradas por aderência no interior do elemento estrutural. Apresentam-se e discutem-se os resultados dos ensaios realizados para avaliar o desempenho das vigas de betão armado reforçadas com esta nova técnica. Os modos de rotura observados são também motivo de análise mais detalhada. Evidenciam-se alguns benefícios na utilização deste sistema de reforço inovador, nomeadamente ao nível da capacidade resistente última das vigas de betão armado e fazem-se algumas recomendações para a sua aplicação e utilização na reabilitação de elementos estruturais degradados.

A reabilitação de estruturas de betão armado com compósitos de FRP tem tido uma grande aceitação em especial devido às excelentes características de durabilidade dos materiais compósitos, ao seu baixo peso e às suas elevadas prestações mecânicas. Contudo, o comportamento elástico-linear dos compósitos de FRP e a sua forma de aplicação pode originar roturas prematuras, quer na técnica de reforço EBR (Externally Bonded Reinforcement), em que o compósito é colado externamente, quer na técnica NSM (Near Surface Mounted) em que o compósito é inserido na zona do recobrimento. No sentido de minimizar o risco de roturas prematuras e ao mesmo tempo aumentar a ductilidade dos elementos reforçados, desenvolveu-se um novo sistema de reforço estrutural em que as armaduras são ancoradas internamente por aderência. A fim de validar o novo sistema de reforço estrutural com compósitos de FRP foi realizado um programa experimental que incluiu o ensaio de vigas de betão armado (BA) reforçadas com as seguintes técnicas de reforço: EBR, NSM e pela nova técnica CREatE (continuous reinforcement embedded at ends). Neste artigo descrevem-se os ensaios experimentais realizados e analisam-se os resultados obtidos. As vigas de BA ensaiadas tinham seção em T, com um vão de 3,0 m e uma altura de 0,3 m e foram solicitadas em flexão em 4 pontos e testadas até a rotura. A técnica CREatE provou ser a mais eficaz das três alternativas testadas mobilizando a totalidade do CFRP e apresentando a maior capacidade resistente e a ductilidade mais elevada.

Measurements in civil engineering load tests usually require considerable time and complex procedures. Therefore, measurements are usually constrained by the number of sensors resulting in a restricted monitored area. Image processing analysis is an alternative way that enables the measurement of the complete area of interest with a simple and effective setup. In this article photo sequences taken during load displacement tests were captured by a digital camera and processed with image correlation algorithms. Three different image processing algorithms were used with real images taken from tests using specimens of PVC and Plexiglas. The data obtained from the image processing algorithms were also compared with the data from physical sensors. A complete displacement and strain map were obtained. Results show that the accuracy of the measurements obtained by photogrammetry is equivalent to that from the physical sensors but with much less equipment and fewer setup requirements.

The wind energy production is a growing industry and the energy produced is renewable and environmentally cleaner than most of the other systems. The towers to support the wind energy generators may be built with precast concrete elements that are competitive in comparison to other structural systems. The evolution of the technology for wind energy production shows a clear need for larger wind turbines and longer blades and, consequently, taller towers, where precast concrete solutions are competitive. Truss precast concrete towers is a solution that complies with the demands of the present and future wind energy production. The research on the execution and structural behaviour of the connections between the tower elements is an important issue. This communication presents the experimental analysis of column to column connections for precast concrete towers. The tests included connections with commercial threaded bars welded to the rebars and connections with grouted corrugated steel sleeves. Thick joints (50 mm) filled with fibre reinforced grout and thin joints (3 mm) filled with epoxy resin were tested and analysed.

This study presents the testing, completion, and analysis of different external strengthening systems applied to rectangular reinforced concrete columns with rounded corners. The experimental study included confinement strengthening with carbon-fiberreinforcedpolymer (CFRP) sheets, longitudinal strengthening with CFRP laminates and confining CFRP jacket, longitudinal strengtheningwith stainless steel bars and confining CFRP jacket, tested column until reinforcing steel failure, repair and CFRP confining jacket,and longitudinal strengthening with stainless steel bars. The analysis of the experimental results included lateral load-displacement relationship, energy dissipation, ductility, and curvature damage assessment classifications. It was concluded that the use of externallongitudinal strengthening combined with CFRP confinement is effective for enhancing performance and is viable in terms of execution. The load capacity increase due to strengthening reached 36–46% with good ductile behavior (failure was reached at 4.4% drift ratio). Despite some differences between CFRP laminates and stainless steel, all columns presented moderate degrees of damage between 1 and 2% drift ratio and minor damage degree until 1% drift ratio.

The peaks over random threshold (PORT) methodology and the Pareto probability weighted moments (PPWM) of the largest observations are used to build a class of location-invariant estimators of the Extreme Value Index (EVI), the primary parameter in statistics of extremes. The asymptotic behaviour of such a class of EVI-estimators, the so-called PORT-PPWM EVI-estimators, is derived, and an alternative class of location-invariant EVI-estimators, the generalized Pareto probability weighted moments (GPPWM) EVI-estimators is considered as an alternative. These two classes of estimators, the PORT-PPWM and the GPPWM, jointly with the classical Hill EVI-estimator and a recent class of minimum-variance reduced-bias estimators are compared for finite samples, through a large-scale Monte-Carlo simulation study. An adaptive choice of the tuning parameters under play is put forward and applied to simulated and real data sets.The peaks over random threshold (PORT) methodology and the Pareto probability weighted moments (PPWM) of the largest observations are used to build a class of location-invariant estimators of the Extreme Value Index (EVI), the primary parameter in statistics of extremes. The asymptotic behaviour of such a class of EVI-estimators, the so-called PORT-PPWM EVI-estimators, is derived, and an alternative class of location-invariant EVI-estimators, the generalized Pareto probability weighted moments (GPPWM) EVI-estimators is considered as an alternative. These two classes of estimators, the PORT-PPWM and the GPPWM, jointly with the classical Hill EVI-estimator and a recent class of minimum-variance reduced-bias estimators are compared for finite samples, through a large-scale Monte-Carlo simulation study. An adaptive choice of the tuning parameters under play is put forward and applied to simulated and real data sets.

In this paper we revisit the Jackson exponentiality test. We study and provide functions in R language to compute theoretical moments, the distribution function and quantiles of the statistic test. Approximations to the exact distribution function and quantiles are also provided and their precision discussed. In addition, we provide an application of the Jackson test to real data.In this paper we revisit the Jackson exponentiality test. We study and provide functions in R language to compute theoretical moments, the distribution function and quantiles of the statistic test. Approximations to the exact distribution function and quantiles are also provided and their precision discussed. In addition, we provide an application of the Jackson test to real data.

For heavy tails, classical extreme value index estimators, like the Hill estimator, are usually asymptotically biased. Consequently those estimators are quite sensitive to the number of top order statistics used in the estimation. The recent minimum-variance reduced-bias extreme value index estimators enable us to remove the dominant component of asymptotic bias and keep the asymptotic variance of the new estimators equal to the asymptotic variance of the Hill estimator. In this paper a new minimum-variance reduced-bias extreme value index estimator is introduced, and its non degenerate asymptotic behaviour is studied. A comparison with another important minimum-variance reduced-bias extreme value index estimator is also provided.For heavy tails, classical extreme value index estimators, like the Hill estimator, are usually asymptotically biased. Consequently those estimators are quite sensitive to the number of top order statistics used in the estimation. The recent minimum-variance reduced-bias extreme value index estimators enable us to remove the dominant component of asymptotic bias and keep the asymptotic variance of the new estimators equal to the asymptotic variance of the Hill estimator. In this paper a new minimum-variance reduced-bias extreme value index estimator is introduced, and its non degenerate asymptotic behaviour is studied. A comparison with another important minimum-variance reduced-bias extreme value index estimator is also provided.

Floods are recurrent events that can have a catastrophic impact. In this work we are interested in the analysis of a data set of gauged daily flows from the Whiteadder Water river, Scotland. Using statistic techniques based on extreme value theory, we estimate several extreme value parameters, including extreme quantiles and return periods of high levels.Floods are recurrent events that can have a catastrophic impact. In this work we are interested in the analysis of a data set of gauged daily flows from the Whiteadder Water river, Scotland. Using statistic techniques based on extreme value theory, we estimate several extreme value parameters, including extreme quantiles and return periods of high levels.

Reduced-bias versions of a very simple generalization of the ‘classical’ Hill estimator of a positive extreme value index (EVI) are put forward. The Hill estimator can be regarded as the logarithm of the mean-of-order-0 of a certain set of statistics. Instead of such a geometric mean, it is sensible to consider the mean-of-order-p (MOP) of those statistics, with p real. Under a third-order framework, the asymptotic behaviour of the MOP, optimal MOP and associated reduced-bias classes of EVI-estimators is derived. Information on the dominant non-null asymptotic bias is also provided so that we can deal with an asymptotic comparison at optimal levels of some of those classes. Large-scale Monte-Carlo simulation experiments are undertaken to provide finite sample comparisons.Reduced-bias versions of a very simple generalization of the ‘classical’ Hill estimator of a positive extreme value index (EVI) are put forward. The Hill estimator can be regarded as the logarithm of the mean-of-order-0 of a certain set of statistics. Instead of such a geometric mean, it is sensible to consider the mean-of-order-p (MOP) of those statistics, with p real. Under a third-order framework, the asymptotic behaviour of the MOP, optimal MOP and associated reduced-bias classes of EVI-estimators is derived. Information on the dominant non-null asymptotic bias is also provided so that we can deal with an asymptotic comparison at optimal levels of some of those classes. Large-scale Monte-Carlo simulation experiments are undertaken to provide finite sample comparisons.