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Teacher education is central to promote the development of the professional knowledge of teachers, and
to help them achieve an appropriate integration of digital technologies, an issue that has proved to be a
difficult one. Several authors refer difficulties in the integration of the technology, emphasizing the central
role played by the teachers’ knowledge in classroom use. In this paper we discuss three models (TPACK
– Technological Pedagogical and Content Knowledge, KTMT – Knowledge for Teaching Mathematics with
Technology, PTK / MPTK - Mathematical Pedagogical Technology Knowledge), intending to identify the
main contributions of each model to a deeper understanding of how to promote the teachers’ integration
of technology in the teaching of Mathematics. The study is based on a literature review and on an analysis
of the similarities and differences among the models and its use. On this analysis we identify common
influences among the models as well as influences from other research areas. The main conclusions
achieved point to a common base to all the models considered, but also to several differences among
them, being that some of the models emphasize the role of technology and its impact on Mathematics
learning, but others go further, intending to integrate in the model elements based on the research on
technology or even other theories such as the one on instrumental genesis.

Perante as conhecidas dificuldades em alcançar uma adequada integração da tecnologia no processo de ensino e aprendizagem da Matemática, este estudo pretende, apoiando-se na formação ao nível da tecnologia ministrada em duas instituições europeias, identificar aspetos com potencial para promover a formação inicial, no âmbito da tecnologia, de professores de Matemática. Adota-se uma metodologia de índole qualitativa e interpretativa, sendo os dados recolhidos de natureza documental ou relativos a trabalhos de análise e reflexão crítica realizados por dois futuros professores (um de cada instituição). As principais conclusões alcançadas apontam para grandes diferenças entre os contextos de formação, com uma das instituições a valorizar de forma mais significativa a formação na área. Ainda assim, os futuros professores de ambas as instituições mostram alguma tendência para escolher tarefas onde a exploração que é feita da tecnologia fica aquém do seu potencial, onde o recurso ao papel e lápis está sempre presente, e onde a reflexão em torno das características das tarefas e da sua implementação parece ser algo superficial. Apesar da complexidade do processo de integração da tecnologia nas práticas, os aspetos referidos parecem-nos ser dignos de atenção em qualquer programa de formação inicial de professores de Matemática.

This chapter aims to make more explicit the grounded or ‘pragmatic theories’ that inform the design of mathematics teachers’ professional development (PD) to exploit technological affordances. It uses aspects of some representative projects that took place in four countries (Colombia, Italy, Mexico, and Portugal) to illustrate lessons learned (e.g., similarities and differences, barriers and opportunities) and provide important insights to inform future PD implementations. To do this, we have identified a set of aspects (and sub-aspects) that emerged in relation to five major themes and reveal our ‘pragmatic theories’ alongside a consideration of the interconnections between these aspects. Our contribution offers a methodological frame to support future PD designs for teachers of mathematics concerning digital technology uses.

After more than one hundred and thirty thousand protein structures determined by X-ray crystallography{,} the challenge of protein crystallization for 3D structure determination remains. In the quest for additives for efficient protein crystallization{,} inorganic materials emerge as an alternative. Magnetic particles (MPs) are versatile inorganic materials{,} easy to use{,} modify and manipulate in a wide range of biological assays. The potential of using functionalised MPs as crystallization chaperones for protein crystallization was shown in this work. MPs with distinct coatings were rationally designed to promote protein crystallization by affinity-triggered heterogeneous nucleation. Hen egg white lysozyme (HEWL) and trypsin{,} were crystallized in the presence of MPs either bare or coated with a polysaccharide (chitin) or a protein (casein){,} respectively. The addition of MPs was characterized in terms of bound protein to the MPs{,} crystal morphology{,} time-lapse of crystal emergence{,} crystallization yield fold change and crystal diffraction quality for structure determination. The MPs additives have shown to bind to the respective target protein{,} and to promote nucleation and crystal growth without compromising crystal morphology. On the other hand{,} MPs addition led to faster detectable crystal emergence and up to 13 times higher crystallization yield{,} addressing some the challenges in protein crystallization{,} the main bottleneck of macromolecular crystallography. Structure determination of the protein crystallized in the presence of MPs revealed that the structural characteristics of the protein remained unchanged{,} as shown by the superposition with PDB annotated proteins. Moreover{,} and unlike most reported cases{,} it was possible to exclude the inhibitor benzamidine during trypsin crystallisation{,} which is a remarkable result opening new prospects in enzyme engineering and drug design. Our results show that MPs coated with affinity ligands to target proteins can be used as controlled and tailor-made crystallization inducers.

Protein crystallization still remains mostly an empirical science, as the production of crystals with the required quality for X-ray analysis is dependent on the intensive screening of the best protein crystallization and crystal’s derivatization conditions. Herein, this demanding step was addressed by the development of a high-throughput and low-budget microfluidic platform consisting of an ion exchange membrane (117 Nafion® membrane) sandwiched between a channel layer (stripping phase compartment) and a wells layer (feed phase compartment) forming 75 independent micro-contactors. This microfluidic device allows for a simultaneous and independent screening of multiple protein crystallization and crystal derivatization conditions, using Hen Egg White Lysozyme (HEWL) as the model protein and Hg2+ as the derivatizing agent. This microdevice offers well-regulated crystallization and subsequent crystal derivatization processes based on the controlled transport of water and ions provided by the 117 Nafion® membrane. Diffusion coefficients of water and the derivatizing agent (Hg2+) were evaluated, showing the positive influence of the protein drop volume on the number of crystals and crystal size. This microfluidic system allowed for crystals with good structural stability and high X-ray diffraction quality and, thus, it is regarded as an efficient tool that may contribute to the enhancement of the proteins’ crystals structural resolution.

Advances in concrete technology during the last decades have resulted in the development of materials with enhanced mechanical properties, such as High Strength Concrete (HSC), Fibre Reinforced Concrete (FRC) and Ultra-High Performance Fibre Reinforced Concrete (UHPFRC). The application of these materials in flat slabs, which are a popular structural solution in Reinforced Concrete (RC) buildings worldwide, has the potential of significantly reducing raw material consumption by enabling the design of slenderer and therefore lighter structures. However, flat slabs are susceptible to punching shear failure, which is a complex phenomenon that remains challenging, even though significant efforts have been made to experimentally study it. For advanced concrete materials (HSC, FRC and UHPFRC), the challenge is further accentuated by the continuous and rapid development of these materials. With the purpose of identifying and highlighting gaps in the published literature, a review of tests with HSC, FRC and UHPFRC slab-column connections in non-seismic and seismic loading applications is presented in this paper. It is shown that future research directions in this field include, among others, testing thicker slabs, HSC slabs with higher concrete compressive strength, HSC combined with FRC and several more cases related to seismic loading conditions.

Being able to efficiently mitigate the effects of blast loads on structures, sacrificial cladding solutions are increasingly used to protect structural elements from the effects of accidental explosions and/or terrorist attacks. The present study analyses the loss of effectiveness of a deterministically designed sacrificial cladding when variability in the material properties and uncertainties in the mechanical model are considered. The results of an experimental campaign are used to validate the numerical models that allow the deterministic design of a sacrificial cladding which successfully improves the blast resistant capabilities of a given structural element. Nonetheless, it is shown that, taking into account the probabilistic variability of key parameters is of vital importance when designing sacrificial cladding solutions, since, when not properly designed for the structural element it intends to protect, adding a sacrificial cladding might negatively impact its blast resistant capabilities. Additionally, it is concluded that the deterministic approach might be against safety. In the reported case study, when comparing the admissible charge weight yielding from the deterministic and probabilistic approaches, one verifies that the former allows a higher charge weight.

Abstract Fiber reinforced polymer (FRP) composites can be efficient for flexural strengthening of flat slabs if debonding of the FRP is postponed. However, with the increase of the flexural capacity, the flat slab becomes more susceptible to punching shear failure. In this context, four flexural or simultaneous flexural and punching shear retrofitting systems are investigated in this study to strengthen a flexure-deficient flat slab. Externally Bonded Reinforcement on Grooves (EBROG) and externally bonded reinforcement (EBR) methods are used for flexural strengthening in two cases: slabs without punching shear reinforcement and with post-installed shear bolts as shear reinforcement. According to the results, flexural strengthening of the slab using the EBR and EBROG techniques increased its load capacity by 12% and 21%, respectively. Simultaneous flexural and shear strengthening of the slab using the EBROG technique was the most effective, leading to a 57% enhancement of the load capacity. For specimens whose failure was governed by punching, comparing the results with code predictions showed that Eurocode and ACI (and the respective guide documents fib bulletin 90 and ACI 440.2R) overestimated the capacity of these specimens. In cases where failure was governed by flexure, a simple application of the yield line theory predicted reasonably well the load capacity of the specimens.

Nuclear Inst. and Methods in Physics Research, A, 1014 (2021) 165701. doi:10.1016/j.nima.2021.165701

The SOUL, or heme-binding protein HBP/SOUL, family represents a group of evolutionary conserved putative heme-binding proteins that contains a number of members in animal, plant andbacterial species. The structures of the murine form of HEBP1, or p22HBP, and the human form of HEBP2, or SOUL, have been determined in 2006 and 2011 respectively. In this work we discuss the structures of HEBP1 and HEBP2 in light of new X-ray data for heme bound murine HEBP1. The interaction between tetrapyrroles and HEBP1, initially proven to be hydrophobic in nature, was thought to also involve electrostatic interactions between heme propionate groups and positively charged amino acid side chains. However, the new X-ray structure, and results from murine HEBP1 variants and human HEBP1, confirm the hydrophobic nature of the heme-HEBP1 interaction, resulting in Kd values in the low nanomolar range, and rules out any electrostatic stabilization. Results from NMR relaxation time measurements for human HEBP1 describe a rigid globular protein with no change in motional regime upon heme binding. X-ray structures deposited in the PDB for human HEBP2 are very similar to each other and to the new heme-bound murine HEBP1 X-ray structure (backbone rmsd ca. 1 {\AA}). Results from a HSQC spectrum centred on the histidine side chain N$δ$-proton region for HEBP2 confirm that HEBP2 does not bind heme via H42 as no chemical shift differences were observed upon heme addition for backbone NH and N$δ$ protons. A survey of the functions attributed to HEBP1 and HEBP2 over the last 20 years span a wide range of cellular pathways. Interestingly, many of them are specific to higher eukaryotes, particularly mammals and a potential link between heme release under oxidative stress and human HEBP1 is also examined using recent data. However, at the present moment, trying to relate function to the involvement of heme or tetrapyrrole binding, specifically, makes little sense with our current biological knowledge and can only be applied to HEBP1, as HEBP2 does not interact with heme. We suggest that it may not be justified to call this very small family of proteins, heme-binding proteins. The family may be more correctly called “the SOUL family of proteins related to cellular fate” as, even though only HEBP1 binds heme tightly, both proteins may be involved in cell survival and/or proliferation.