This paper presents a pulse amplitude modulated signal generator to address inference in Memristor based Neural Network Accelerators. As a part of this system, a novel 8-bit capacitive segmented Digital to Analog Converter (DAC) using amorphous Indium Galium Zinc Oxide (a-IGZO) thin-film transistor (TFT) technology has been designed. The DAC employs 50% segmentation with binary coded least significant bits (LSBs) and unary coded most significant bits (MSBs). This circuit has shown an ENOB of 7.3 bits at a sampling frequency of 100 kHz and an input frequency of 50 kHz. The worst case INL and DNL were recorded as 0.047 LSB and 0.34 LSB, respectively. With a power supply voltage of 5 V for the operational amplifier and 3V as the DAC reference voltage, the power consumption of the complete DAC was around 1.25 mW. This circuit can find potential applications in different flexible electronics systems.

Neisseria gonorrhoeae is an obligate human pathogenic bacterium responsible for gonorrhea, a sexually transmitted disease. The bacterial peroxidase, an enzyme present in the periplasm of this bacterium, detoxifies the cells against hydrogen peroxide and constitutes one of the primary defenses against exogenous and endogenous oxidative stress in this organism. The 38 kDa heterologously produced bacterial peroxidase was crystallized in the mixed-valence state, the active state, at pH 6.0, and the crystals were soaked with azide, producing the first azide-inhibited structure of this family of enzymes. The enzyme binds exogenous ligands such as cyanide and azide, which also inhibit the catalytic activity by coordinating the P heme iron, the active site, and competing with its substrate, hydrogen peroxide. The inhibition constants were estimated to be 0.4 ± 0.1 µM and 41 ± 5 mM for cyanide and azide, respectively. Imidazole also binds and inhibits the enzyme in a more complex mechanism by binding to P and E hemes, which changes the reduction potential of the latest heme. Based on the structures now reported, the catalytic cycle of bacterial peroxidases is revisited. The inhibition studies and the crystal structure of the inhibited enzyme comprise the first platform to search and develop inhibitors that target this enzyme as a possible new strategy against N. gonorrhoeae.

The cellulosome is an elaborate multi-enzyme structure secreted by many anaerobic microorganisms for the efficient degradation of lignocellulosic substrates. It is composed of multiple catalytic and non-catalytic components that are assembled through high-affinity protein-protein interactions between the enzyme-borne dockerin (Doc) modules and the repeated cohesin (Coh) modules present in primary scaffoldins. In some cellulosomes, primary scaffoldins can interact with adaptor and cell-anchoring scaffoldins to create structures of increasing complexity. The cellulosomal system of the ruminal bacterium, Ruminococcus flavefaciens, is one of the most intricate described to date. An unprecedent number of different Doc specificities results in an elaborate architecture, assembled exclusively through single-binding-mode type-III Coh-Doc interactions. However, a set of type-III Docs exhibits certain features associated with the classic dual-binding mode Coh-Doc interaction. Here, the structure of the adaptor scaffoldin-borne ScaH Doc in complex with the Coh from anchoring scaffoldin ScaE is described. This complex, unlike previously described type-III interactions in R. flavefaciens, was found to interact in a dual-binding mode. The key residues determining Coh recognition were also identified. This information was used to perform structure-informed protein engineering to change the electrostatic profile of the binding surface and to improve the affinity between the two modules. The results show that the nature of the residues in the ligand-binding surface plays a major role in Coh recognition and that Coh-Doc affinity can be manipulated through rational design, a key feature for the creation of designer cellulosomes or other affinity-based technologies using tailored Coh-Doc interactions.

This paper investigates the implementation of the Extended-Matsuoka–Nakai yield criterion on a strict Limit Analysis finite element formulation. The current approach is based on a three-field mixed finite element model and the Alternating Direction Method of Multipliers optimization algorithm. With the support of duality principles two variants are derived, the lower bound and the upper bound element. The main novelty of this work is the development of an efficient iterative predictor–corrector scheme, customized for the Extended-Matsuoka–Nakai. This scheme is an indispensable requirement for this formulation. To conclude four numerical examples are presented to assess the effectiveness and efficiency of the numerical tool.

A numerical implementation of the upper-bound theorem of limit analysis is applied to determine two-dimensional (2D) and three-dimensional (3D) active horizontal earth pressure coefficients considering seismic actions through a horizontal seismic coefficient. Results are obtained for vertical wall, horizontal soil, different friction angles of the soil, soil-to-wall friction ratios, horizontal seismic coefficients and wall width-to-height ratios. The few cases for which 3D active earth pressure coefficients are available in the literature using upper-bound methods were used for comparison with the corresponding earth pressure coefficients obtained in this study. This showed a general improvement of these results, which allows expecting a good accuracy for the set of cases studied. The ratios between the 3D and 2D horizontal active earth pressure coefficients are found to be practically independent of the soil-to-wall friction ratio. An equation is proposed for calculating these ratios. This equation can be easily used in the design of geotechnical structures requiring the determination of 3D active earth pressure coefficients.

Laser-induced graphene (LIG) is as a promising material for flexible microsupercapacitors (MSCs) due to its simple and cost-effective processing. However, LIG-MSC research and production has been centered on non-sustainable polymeric substrates, such as polyimide. In this work, it is presented a cost-effective, reproducible, and robust approach for the preparation of LIG structures via a one-step laser direct writing on chromatography paper. The developed strategy relies on soaking the paper in a 0.1 M sodium tetraborate solution (borax) prior to the laser processing. Borax acts as a fire-retardant agent, thus allowing the laser processing of sensitive substrates that other way would be easily destroyed under the high-energy beam. LIG on paper exhibiting low sheet resistance (30 $Ømega$ sq−1) and improved electrode/electrolyte interface was obtained by the proposed method. When used as microsupercapacitor electrodes, this laser-induced graphene resulted in specific capacitances of 4.6 mF cm−2 (0.015 mA cm−2). Furthermore, the devices exhibit excellent cycling stability (> 10,000 cycles at 0.5 mA cm−2) and good mechanical properties. By connecting the devices in series and parallel, it was also possible to control the voltage and energy delivered by the system. Thus, paper-based LIG-MSC can be used as energy storage devices for flexible, low-cost, and portable electronics. Additionally, due to their flexible design and architecture, they can be easily adapted to other circuits and applications with different power requirements. Graphical Abstract: [Figure not available: see fulltext.]