The Orange Protein (ORP) is a small bacterial protein, of unknown function, that harbors a unique molybdenum/copper (Mo/Cu) heterometallic cluster, [S2MoVIS2CuIS2MoVIS2]3-, noncovalently bound. The apo-ORP is able to promote the formation and stabilization of this cluster, using CuII- and MoVIS42- salts as starting metallic reagents, to yield a Mo/Cu-ORP that is virtually identical to the native ORP. In this work, we explored the ORP capability of promoting protein-assisted synthesis to prepare novel protein derivatives harboring molybdenum heterometallic clusters containing iron, cobalt, nickel, or cadmium in place of the "central" copper (Mo/Fe-ORP, Mo/Co-ORP, Mo/Ni-ORP, or Mo/Cd-ORP). For that, the previously described protein-assisted synthesis protocol was extended to other metals and the Mo/M-ORP derivatives (M = Cu, Fe, Co, Ni, or Cd) were spectroscopically (UV-visible and electron paramagnetic resonance (EPR)) characterized. The Mo/Cu-ORP and Mo/Cd-ORP derivatives are stable under oxic conditions, while the Mo/Fe-ORP, Mo/Co-ORP, and Mo/Ni-ORP derivatives are dioxygen-sensitive and stable only under anoxic conditions. The metal and protein quantification shows the formation of 2Mo:1M:1ORP derivatives, and the visible spectra suggest that the expected {S2MoS2MS2MoS2} complexes are formed. The Mo/Cu-ORP, Mo/Co-ORP, and Mo/Cd-ORP are EPR-silent. The Mo/Fe-ORP derivative shows an EPR S = 3/2 signal (E/D approximately 0.27, g approximately 5.3, 2.5, and 1.7 for the lower M= +/-1/2 doublet, and g approximately 5.7 and 1.7 (1.3 predicted) for the upper M = +/-3/2 doublet), consistent with the presence of either one S = 5/2 FeIII antiferromagnetically coupled to two S = 1/2 MoV or one S = 3/2 FeI and two S = 0 MoVI ions, in both cases in a tetrahedral geometry. The Mo/Ni-ORP shows an EPR axial S = 1/2 signal consistent with either one S = 1/2 NiI and two S = 0 MoVI or one S = 1/2 NiIII antiferromagnetically coupled to two S = 1/2 MoV ions, in both cases in a square-planar geometry. The Mo/Cu-ORP and Mo/Cd-ORP are described as {MoVI-CuI-MoVI} and {MoVI-CdII-MoVI}, respectively, while the other derivatives are suggested to exist in at least two possible electronic structures, {MoVI-MI-MoVI} <--> {MoV-MIII-MoV}.

Fiber Reinforced Polymers (FRP) is a recent technique to strengthen timber structures and the studies available discussing the debonding between these materials are limited. Therefore, the bond assessment between FRP composites and timber substrates is a topic that needs clarification. The present work analyses the debonding process between Carbon (C) FRP laminates and timber with rupture modes consistent with Mode II interfacial fracture, i.e. with the sliding mode where the bond stresses act parallel to the plane of the bonding surface. Several single-lap shear tests were performed and the experiments showed a nonlinear local behaviour of the CFRP-to-timber interface. An interfacial bond-slip model and its calibration procedure were also presented. Furthermore, the calibrated nonlinear bond-slip model was implemented in a numerical approach where the FRP composite and the adhesive are simulated by linear and nonlinear springs and the substrate is assumed rigid. The following influences on the debonding process of the CFRP-to-timber interface were also analysed: (i) the bonding technique (Externally Bonded Reinforcement - EBR; and Near Surface Mounted - NSM); and (ii) the use of an additional device to mechanically anchor the CFRP laminate. Besides the determination of the effective bond length for each bonding technique, a new concept defining the length beyond which the force at the anchorage device does not decrease with the bonded length and a proposal to estimate its value for any bonded length was also presented and discussed. The experimental tests have shown that the NSM technique has a better performance compared to the EBR technique, independently of the installation of mechanical anchorage devices. In the case of the EBR technique, the strains in the CFRP laminate increased at its vicinities due to the clamping force applied to the anchors, which affected the final strength of the interface.

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The development of meaningful learning becomes possible when students are actively involved in solving real problems. Thus, this study intends to investigate how students of the 11th grade of a vocational course solve problems of Linear Programming, using the graphing calculator. The conclusions reached indicate that: the interpretation of the conditions of the problems is the most delicate point; the graphical approach using technology is dominant; and the difficulties raised by the problem as well as the need to discuss the results achieved are the basis for the interactions both among the students and between them and the teacher.

http://docentes.fct.unl.pt/lblm/files/inorgchem-2017-56-2210.pdf

Este livro apresenta diferentes perspetivas sobre o património arquitetónico em Espanha e Itália, debruçando-se sobre temas que estão na ordem do dia, como a reabilitação do património arquitetónico ou as acessibilidades ao mesmo, sejam elas na forma física ou digital, sendo os autores profundos conhecedores desta área. Nas intervenções a realizar no património edificado é fundamental ter um conhecimento aprofundado do projeto, da história e do processo construtivo da obra, bem como do seu comportamento estrutural. De acordo com o ICOMOS estas intervenções devem ser realizadas utilizando as técnicas mais adequadas, baseadas num diagnóstico apropriado e na compreensão dos materiais existentes. De igual forma, os estudos no edificado monumental devem ser realizados com o menor grau de intrusão e o máximo respeito pela sua integridade física, seguindo os princípios de salvaguarda do património arquitetónico definidos na carta internacional de Atenas.O livro divide‐se em duas grandes áreas: numa, abordam‐se os assuntos que se enquadram essencialmente no âmbito da conservação e reabilitação do património arquitetónico e na outra são apresentados os temas mais dedicados à acessibilidade do público ao património arquitetónico e à sua divulgação online. Os trabalhos apresentados no âmbito da conservação e reabilitação do património arquitetónico seguem na essência as recomendações do ICOMOS em relação à necessidade de compreensão da construção existente, ao desenvolvimento de novas técnicas e dos materiais mais adequados aos trabalhos de conservação e reabilitação, assim como na realização de testes em modelos experimentais à escala, tendo por objetivo avaliar o comportamento da estrutura antes e após a reabilitação. O património arquitetónico construído constitui uma parte importante da história da civilização humana e é um fator determinante da nossa identidade coletiva que importa preservar para as gerações futuras. Com a sua intervenção na conservação, reabilitação, valorização e divulgação do património construído, os autores deste livro estão a cumprir um importante papel na nossa sociedade, preservando um legado que nos foi deixado pelos nossos antepassados.

The importance of understanding interactomes makes preeminent the study of protein interactions and protein complexes. Traditionally, protein interactions have been elucidated by experimental methods or, with lower impact, by simulation with protein docking algorithms. This article describes features and applications of the BiGGER docking algorithm, which stands at the interface of these two approaches. BiGGER is a user-friendly docking algorithm that was specifically designed to incorporate experimental data at different stages of the simulation, to either guide the search for correct structures or help evaluate the results, in order to combine the reliability of hard data with the convenience of simulations. Herein, the applications of BiGGER are described by illustrative applications divided in three Case Studies: (Case Study A) in which no specific contact data is available; (Case Study B) when different experimental data (e.g., site-directed mutagenesis, properties of the complex, NMR chemical shift perturbation mapping, electron tunneling) on one of the partners is available; and (Case Study C) when experimental data are available for both interacting surfaces, which are used during the search and/or evaluation stage of the docking. This algorithm has been extensively used, evidencing its usefulness in a wide range of different biological research fields.