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Carvalho, Ana Luísa, Teresa Santos-Silva, Maria João Romão, J. Eurico, and Filipa Marcelo. "{CHAPTER 2 Structural Elucidation of Macromolecules}." Essential Techniques for Medical and Life Scientists: A Guide to Contemporary Methods and Current Applications with the Protocols. BENTHAM SCIENCE PUBLISHERS, 2018. 30-91. Abstract

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Carvalho, Ana Luisa, Fernando M. V. Dias, Tibor Nagy, Jose A. M. Prates, Mark R. Proctor, Nicola Smith, Edward A. Bayer, Gideon J. Davies, Luis M. A. Ferreira, Maria J. Romao, Carlos M. G. A. Fontes, and Harry J. Gilbert. "Evidence for a dual binding mode of dockerin modules to cohesins." Proceedings of the National Academy of Sciences of the United States of America. 104 (2007): 3089-3094. Abstract
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Carvalho, AL, A. Goyal, JAM Prates, DN Bolam, HJ Gilbert, VMR Pires, LMA Ferreira, A. Planas, MJ Romao, and CMGA Fontes. "The family 11 carbohydrate-binding module of Clostridium thermocellum Lic26A-Cel5E accommodates beta-1,4- and beta-1,3-1,4-mixed linked glucans at a single binding site." Journal of Biological Chemistry. 279 (2004): 34785-34793. Abstract
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Carvalho, AL, VMR Pires, TM Gloster, JP Turkenburg, JAM Prates, LMA Ferreira, MJ Romao, GJ Davies, CMGA Fontes, and HJ Gilbert. "Insights into the structural determinants of cohesin dockerin specificity revealed by the crystal structure of the type II cohesin from Clostridium thermocellum SdbA." Journal of Molecular Biology. 349 (2005): 909-915. Abstract
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Carvalho, AL, JM Dias, L. Sanz, A. Romero, JJ Calvete, and MJ Romao. "Purification, crystallization and identification by X-ray analysis of a prostate kallikrein from horse seminal plasma." Acta Crystallographica Section D-Biological Crystallography. 57 (2001): 1180-1183. Abstract

The purification, crystallization and identification by X-ray diffraction analysis of a horse kallikrein is reported. The protein was purired from horse seminal plasma. Crystals belong to space group C2 and the structure was solved by the MIRAS method, with two heavy-atom derivatives of mercury and platinum. X-ray diffraction data to 1.42 Angstrom resolution were collected at the ESRF synchrotron-radiation source.

Carvalho, Ana Luísa, José Trincão, and Maria João Romão. "X-Ray Crystallography in Drug Discovery." Methods in molecular biology (Clifton, N.J.). Vol. 572. 2010. 31-56. Abstract

Macromolecular X-ray crystallography is an important and powerful technique in drug discovery, used by pharmaceutical companies in the discovery process of new medicines. The detailed analysis of crystal structures of protein-ligand complexes allows the study of the specific interactions of a particular drug with its protein target at the atomic level. It is used to design and improve drugs. The starting point of these studies is the preparation of suitable crystals of complexes with potential ligands, which can be achieved by using different strategies described in this chapter. In addition, an introduction to X-ray crystallography is given, highlighting the fundamental steps necessary to determine the three-dimensional structure of protein-ligand complexes, as well as some of the tools and criteria to validate crystal structures available in databases.

Carvalho, AL, L. Sanz, D. Barettino, A. Romero, JJ Calvete, and MJ Romao. "Crystal structure of a prostate kallikrein isolated from stallion seminal plasma: A homologue of human PSA." Journal of Molecular Biology. 322 (2002): 325-337. Abstract
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Carvalho, AL, FMV Dias, JAM Prates, T. Nagy, HJ Gilbert, GJ Davies, LMA Ferreira, MJ Romao, and CMGA Fontes. "Cellulosome assembly revealed by the crystal structure of the cohesin-dockerin complex." Proceedings of the National Academy of Sciences of the United States of America. 100 (2003): 13809-13814. Abstract
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Coelho, Catarina, Pablo J. Gonzalez, Jose Trincao, Ana L. Carvalho, Shabir Najmudin, Thomas Hettman, Stephan Dieckman, Jose J. G. Moura, Isabel Moura, and Maria J. Romao. "Heterodimeric nitrate reductase (NapAB) from Cupriavidus necator H16: purification, crystallization and preliminary X-ray analysis." Acta Crystallographica Section F-Structural Biology and Crystallization Communications. 63 (2007): 516-519. Abstract
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Correia, Viviana G., Benedita A. Pinheiro, Ana Luísa Carvalho, and Angelina S. Palma. "Resistance to Aminoglycosides." Antibiotic Drug Resistance. John Wiley & Sons, Ltd, 2019. 1-38. Abstract

Summary The emergence of bacterial resistance to different antibiotics in clinical use, together with the knowledge on the mechanisms by which bacteria resist the action of aminoglycosides, have contributed to the renewed interest in these molecules as potential antimicrobials. Here, we give an overview on natural and semisynthetic aminoglycosides and their structural features and modes of action, focusing on the structural insight underlying resistance mechanisms. Developments on carbohydrate chemistry and microarray technology are highlighted as powerful approaches toward generation of new aminoglycosides and for screening their interactions with RNAs and proteins. The link between antibiotic uptake and the human gut microbiome is also addressed, focusing on gut microbiome function and composition, antibiotic-induced alterations in host health, and antibiotic resistance. In addition, strategies to modulate human microbiome responses to antibiotics are discussed as novel approaches for aminoglycoside usage and for the effectiveness of antibiotic therapy.