The electron transfer complex between nitrous oxide reductase and its electron donors

Citation:
Dell'acqua, S., I. Moura, J. J. Moura, and S. R. Pauleta. "The electron transfer complex between nitrous oxide reductase and its electron donors." J Biol Inorg Chem. 16 (2011): 1241-54.

Abstract:

Identifying redox partners and the interaction surfaces is crucial for fully understanding electron flow in a respiratory chain. In this study, we focused on the interaction of nitrous oxide reductase (N(2)OR), which catalyzes the final step in bacterial denitrification, with its physiological electron donor, either a c-type cytochrome or a type 1 copper protein. The comparison between the interaction of N(2)OR from three different microorganisms, Pseudomonas nautica, Paracoccus denitrificans, and Achromobacter cycloclastes, with their physiological electron donors was performed through the analysis of the primary sequence alignment, electrostatic surface, and molecular docking simulations, using the bimolecular complex generation with global evaluation and ranking algorithm. The docking results were analyzed taking into account the experimental data, since the interaction is suggested to have either a hydrophobic nature, in the case of P. nautica N(2)OR, or an electrostatic nature, in the case of P. denitrificans N(2)OR and A. cycloclastes N(2)OR. A set of well-conserved residues on the N(2)OR surface were identified as being part of the electron transfer pathway from the redox partner to N(2)OR (Ala495, Asp519, Val524, His566 and Leu568 numbered according to the P. nautica N(2)OR sequence). Moreover, we built a model for Wolinella succinogenes N(2)OR, an enzyme that has an additional c-type-heme-containing domain. The structures of the N(2)OR domain and the c-type-heme-containing domain were modeled and the full-length structure was obtained by molecular docking simulation of these two domains. The orientation of the c-type-heme-containing domain relative to the N(2)OR domain is similar to that found in the other electron transfer complexes.

Notes:

Dell'acqua, SimoneMoura, IsabelMoura, Jose J GPauleta, Sofia RResearch Support, Non-U.S. Gov'tGermanyJournal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic ChemistryJ Biol Inorg Chem. 2011 Dec;16(8):1241-54. doi: 10.1007/s00775-011-0812-9. Epub 2011 Jul 8.

Related External Link