Jan 2014 - Principal Researcher UCIBIO - REQUIMTE

I am a Principal Researcher in the Associated Laboratory, UCIBIO@REQUIMTE.

My research is focused on the structural and mechanistic studies of metalloenzymes isolated from Paracoccus pantotrophus, Marinobacter hydrocarbonoclasticus and from sulphate reducing bacteria of the genus Desulfovibrio. A more recent research area is the microbial stress response to oxidative stress and metal ions.

Main interests include physical biochemistry and structural biology (NMR) of electron transfer proteins and enzymes, as well as, other metalloproteins. Expertise in multitude of biophysical (ITC and analytical ultracentrifugation) and spectroscopic techniques (UV-vis, Raman, Fluorescence, CD and NMR). NMR expertise include solution structure determination of proteins using heteronuclear experiments (and the used of 13C-direct detection experiments) and the study of protein-protein interactions and more recently protein-DNA interactions.

- Structural and mechanistic studies of metalloenzymes and electron transfer complexes using different biochemical and biophysical techniques (cytochrome c peroxidase and superoxide reductase and its redox partners, pseudoazurin, cytochrome c550, and rubredoxin, respectively, and more recently nitrous oxide reductase).

- Solution structure determination using NMR. Use of NMR to characterize the dynamics of proteins in solution using the analysis of 15N relaxation data (R1, R2 and NOE): study of novel molybdenum cofactor containing proteins.

- Proteomics and gene expression profile constitute a recent additional area of interest applied to the study of microbial stress response to metals (Cu, Co, Ag) and to oxidative stress.

Contacts

Gab. 632B, Dep. Química

Tel. + 351 212 948 385, ext 10967
Fax + 351 2112 948 550

UCIBIO, REQUIMTE
Dep. Química, FCT/UNL
2829-516 Caparica
Portugal

The lab is located in UCIBIO, REQUIMTE in the Faculdade de Ciências e Tecnologia - Universidade Nova de Lisboa, Campus da Caparica

Address
UCIBIO, REQUIMTE
Departamento de Química
Faculdade de Ciênicas e Tecnologia
Universidade Nova de Lisboa
Campus da Caparica
2829-516 Caparica
Portugal

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