The p53 tumor suppressor is widely found to be mutated in human cancer. This protein is regarded as a molecular hub regulating different cell responses, namely cell death. Compelling data have demonstrated that the impairment of p53 activity correlates with tumor development and maintenance. For these reasons, the reactivation of p53 function is regarded as a promising strategy to halt cancer. In the present work, the recombinant mutant p53R280K DNA binding domain (DBD) was produced for the first time, and its crystal structure was determined in the absence of DNA to a resolution of 2.0 Å. The solved structure contains four molecules in the asymmetric unit, four zinc(II) ions, and 336 water molecules. The structure was compared with the wild-type p53 DBD structure, isolated and in complex with DNA. These comparisons contributed to a deeper understanding of the mutant p53R280K structure, as well as the loss of DNA binding related to halted transcriptional activity. The structural information derived may also contribute to the rational design of mutant p53 reactivating molecules with potential application in cancer treatment.

The compounds 1-naphthyliminoferrocene{,} 1{,} and 2-methyl-1-naphthyliminoferrocene{,} 2{,} were prepared by the reaction of ferrocenecarboxaldehyde and the corresponding amine{,} 1-naphthylamine and 2-methyl-1-naphthylamine with one drop of pyrrolidine as catalyst{,} in CH2Cl2{,} under inert atmosphere. The compounds were fully characterized by elemental analyses{,} matrix-assisted laser desorption/ionization - time-of-flight mass spectrometry (MALDI-TOF){,} FT-IR{,} 1H and 13C NMR as well as two-dimensional{,} NOESY{,} COSY and HSQC{,} NMR spectroscopy. Mossbauer spectroscopic studies were performed to characterize the iron species in both compounds. The solid-state molecular structures of compounds 1 and 2 were determined by single crystal X-ray diffraction. Analysis of the electrochemical behaviour of complexes 1 and 2 was performed by cyclic and square wave voltammetry. Compounds 1 and 2 were tested as chromogenic and electrochemical sensors for Hg2+.

The functional and biological significance of the selected CASP12 targets are described by the authors of the structures. The crystallographers discuss the most interesting structural features of the target proteins and assess whether these features were correctly reproduced in the predictions submitted to the CASP12 experiment. This article is protected by copyright. All rights reserved.

It is known that zinc alloys with iron group metals have better corrosion resistance than pure zinc. Owing to the corrosion resistance of these alloys, Zn–Fe coatings are widely used in automotive industry...

Spectrochimica Acta Part B: Atomic Spectroscopy, 130 (2017) 60-66. doi:10.1016/j.sab.2017.02.012

Phylogenetic analyses of morphological data are often characterized by missing data due to incomplete operational taxonomic units, as in fossils. This incomplete knowledge derives from various reasons, including—in the case of fossils—the numerous filters an organism has to pass through during taphonomy, fossilization, weathering and collecting. Whereas several methods have been proposed to address issues raised by the inclusion of incomplete terminal taxa, until recently no tool existed to easily quantify the amount of anatomical overlap within a particular clade. The Overlap Indices provide such values and might prove useful for comparative cladistics. We herein describe these new indices and their applications in detail and provide an example file for their calculation. A case study of diplodocid sauropod dinosaurs shows how the Overlap Indices will help to explore and quantify, which one of a number of conflicting tree topologies is supported by more anatomical traits, which skeletal regions are underrepresented in a particular phylogenetic matrix, and which taxon would improve character state score completeness.

Precast concrete sandwich panels started being used as cladding for buildings, together with the rise of industrial prefabrication, during the mid-20th century. Since then, society and industry have become increasingly aware of energy efficiency in all fields, for both affordability and sustainability consciousness. As such, buildings have been subject to increasingly stringent requirements with the technology of sandwich panels kept continually at the forefront.
Nowadays, sandwich panels have reached the highest standards of functional performance as structural efficiency, flexibility in use, the speed as well as of aesthetic appeal. These combine in building construction with the well-known advantages of prefabrication; such as construction, quality consciousness, durability and sustainability. Sandwich panels have gained more and more important in their field, thus representing quite a significant application within the industry of prefabrication and an important share of the market.
The Commission ‘Prefabrication’ is keen to promote the development of all precast structural concrete products and to transfer the knowledge to practical design and construction. Now filling a strategic gap, by issuing this Guide to Good Practice, which includes design considerations, structural analysis, building physics, use of materials, manufacturing methods, equipment, field performance, and provides a comprehensive overview of the information currently available worldwide. The Commission is particularly proud that this document is a result of close cooperation with PCI and that it will be published by both fib and PCI. This cooperation started six years ago, first with comparing the different approaches to several issues, then progressively integrating up to producing common documents, like this one, that wasn’t yet treated in a specific Guide by either body.

Diplodocids are among the best known sauropod dinosaurs. Numerous specimens of currently 15 accepted species belonging to ten genera have been reported from the Late Jurassic to Early Cretaceous of North and South America, Europe, and Africa. The highest diversity is known from the Upper Jurassic Morrison Formation of the western United States: a recent review recognized 12 valid, named species, and possibly three additional, yet unnamed ones. One of these is herein described in detail and referred to the genus \textit{Galeamopus}. The holotype specimen of \textit{Galeamopus pabsti} sp. nov., SMA 0011, is represented by material from all body parts but the tail, and was found at the Howe-Scott Quarry in the northern Bighorn Basin in Wyoming, USA. Autapomorphic features of the new species include a horizontal canal on the maxilla that connects the posterior margin of the preantorbital and the ventral margin of the antorbital fenestrae, a vertical midline groove marking the sagittal nuchal crest, the presence of a large foramen connecting the postzygapophyseal centrodiapophyseal fossa and the spinopostzygapophyseal fossa of mid- and posterior cervical vertebrae, a very robust humerus, a laterally placed, rugose tubercle on the concave proximal portion of the anterior surface of the humerus, a relatively stout radius, the absence of a distinct ambiens process on the pubis, and a distinctly concave posteroventral margin of the ascending process of the astragalus. In addition to the holotype specimen SMA 0011, the skull USNM 2673 can also be referred to \textit{Galeamopus pabsti}. Histology shows that the type specimen SMA 0011 is sexually mature, although neurocentral closure was not completed at the time of death. Because SMA 0011 has highly pneumatized cervical vertebrae, the development of the lamination appears a more important indicator for individual age than neurocentral fusion patterns. SMA 0011 is one of very few sauropod specimens that preserves the cervico-dorsal transition in both vertebrae and ribs. The association of ribs with their respective vertebrae shows that the transition between cervical and dorsal vertebrae is significantly different in \textit{Galeamopus pabsti} than in \textit{Diplodocus carnegii} or \textit{Apatosaurus louisae}, being represented by a considerable shortening of the centra from the last cervical to the first dorsal vertebra. Diplodocids show a surprisingly high diversity in the Morrison Formation. This can possibly be explained by a combination of geographical and temporal segregation, and niche partitioning.

Phylogenetic analyses of morphological data are often characterized by missing data due to incomplete operational taxonomic units, as in fossils. This incomplete knowledge derives from various reasons, including—in the case of fossils—the numerous filters an organism has to pass through during taphonomy, fossilization, weathering and collecting. Whereas several methods have been proposed to address issues raised by the inclusion of incomplete terminal taxa, until recently no tool existed to easily quantify the amount of anatomical overlap within a particular clade. The Overlap Indices provide such values and might prove useful for comparative cladistics. We herein describe these new indices and their applications in detail and provide an example file for their calculation. A case study of diplodocid sauropod dinosaurs shows how the Overlap Indices will help to explore and quantify, which one of a number of conflicting tree topologies is supported by more anatomical traits, which skeletal regions are underrepresented in a particular phylogenetic matrix, and which taxon would improve character state score completeness.

This paper presents a comparative evaluation of efficiencies of different accelerated ageing tests (freezethaw, thermal shock, salt crystallization, dissolution and wetting-drying) and fuzzy inference system in predicting weathering degrees of some monument stones from three historical sites (Anahita Temple, Anobanini reliefs and Eshkaft-e Salman reliefs, Iran). The combined effects of natural weathering processes (heating and cooling, wetting and drying, and freezing and thawing) and climatic information were used for assessing the natural weathering degrees. Finally, the natural weathering degrees were multiplied by time effect coefficients to obtain more realistic natural weathering degrees of the monuments. The predicted natural weathering degrees for Anahita Temple, Anobanini reliefs and Eshkaft-e Salman reliefs are 56%, 61%, and 47%, respectively. These predicted values reasonably support the weathering degrees defined by progressive decay indices (calculated equal to 2.77, 3.42 and 2.66 for Anahita Temple, Anobanini reliefs and Eshkaft-e Salman reliefs, respectively), which means the fuzzy model potentially could accurately predict the weathering of stones.

Weathering imposes vital effects on stony monuments. Mostly, the degree of weathering is determined by simple test results, ignoring simultaneous effects of various weathering factors. Hence, the main purpose of this study is to develop prediction models with fuzzy inference systems to determine the weathering degree of the Persepolis stone, using various accelerated ageing tests in laboratory condition and to extrapolate the results to the natural condition, considering climatic information. The results suggest reliable conformity between the prediction of the weathering degree of the stone and the weathering degree observed in the Persepolis complex in natural condition.

Biodegradable polymers such as PLA have been studied for medical applications, human ligament repair is one of such cases. However, these materials can be applied in other sectors as aerospace, aeronautics, automotive, food packaging. PLA presents a relatively brittle on the mode I fracture behavior, being often blend with other biodegradable or non-degradable polymers to improve its fracture energy. For some existing applications, PLA components exhibit accumulated permanent deformation resulting from dynamic mechanical inputs, resulting on failure by laxity of parts. Aiming the improvement of PLA mechanical properties, the inclusion of carbon nanofillers into PLA matrix, in particular, CNT-COOH and GNP have been developed, due to their strong sp2 carbon-carbon bondings and their geometric arrangement that enhance mechanical properties of the polymer matrix. PLA and nanocomposites were produced by melt blending followed by compression moulding in a hot press, with small weight percentages of nanofillers added to the matrix. Nanocomposites dispersion was evaluated by SEM. Quasi static tensile tests were performed on a mechanical testing machine (Instron� ElectroPuls E1000) along with strain field measurements of specimens with centred crack with digital image correlation, revealing strain distribution along specimens.