The use of FRP composites for the confinement of concrete has become an important aspect to consider on strengthening of concrete columns. It is important therefore that accurate modelling tools are available for the design of this system considering, not only the peak values of load and strain, but also the complete stress–strain behaviour. A wide group of authors have proposed several models specific for FRP-confined concrete based either on theoretical assumptions (analysis-oriented-models – AOMs) or on mathematical calibration from testing results (design-oriented-models – DOMs). This article carries out the implementation and analysis of nine existing models for circular concrete columns in view of axially tested reinforced concrete columns confined with CFRP with three different diameters: 150; 250 and 400 mm. The global shape of curves, peak compressive load, stress–strain relation, axial-to-lateral relation and dilation response were studied to conclude which models’ curves were closer to tests. Quantification of errors in face of the testing results was carried out for the most important parameters – ultimate load, strain and lateral stress – as well as for other curve parameters. Some models are accurate in predicting the peak load, though only few can accurately predict the load–strain and dilation behaviour.

This paper describes for the first time the intimate molecular details of the association between a platinated oligonucleotide and a zinc-finger peptide. Site-specific platination of the guanine in a ss hexanucleotide gave {[Pt(dien)d(5’-TACGCC-3’)], Pt(dien)(6-mer)}, II, characterized by mass spectrometry and 1H-NMR spectroscopy. The work extends the study of platinum-nucleobase complex-zinc finger interactions using small molecules such as [Pt(dien)(9-EtGua)]2+, I . The structure of the (34-52) C-terminal finger of the HIV nucleocapsid protein HIVNCp7 (ZF1) was characterized by 1H-NMR spectroscopy and compared with that of the N-terminal single finger and the 2-finger “intact” NCp7. Interaction of II with ZF1 results in significant changes in comparison to the “free” uncomplexed hexanucleotide – the major shifts occur for Trp37 resonances are broadened and shifted upfield and other major shifts are for Gln45 (H21, H3, Q), Met46 (NH, H2), Lys47 (NH, Q) and Glu50 (H2, H3). The Zn-Cys/His chemical shifts show only marginal deviations. The solution structure of ZF1, the 6-mer/ZF1 and II/ZF1 adducts were calculated from the NOESY-derived distance constraints. The DNA position in II/ZF1 is completely different than in the absence of platinum. Major differences are the appearance of new Met46-Cyt6H5 and Trp37-Cyt5H5 contacts but severe weakening of the Trp37-Gua4 contact, attributed to the steric effects caused by Gua4 platination, accompanied by a change in the position of the aromatic ring. The results demonstrate the feasibility of targetting specific ZF motifs with DNA-tethered coordination compounds, such as Pt compounds and Co-macrocycles – with implications for drug targetting and indeed the intimate mechansims of DNA repair of platinated DNA.

The basal part of the Triassic-Jurassic (Rhaetian-Sinemurian) Kap Stewart Formation, exposed at Jameson Land, East Greenland, yields an extensive coprolite collection from black, parallel-laminated mudstone (“paper shale”), representing an open lacustrine system. Preliminary investigations show three different types of coprolites: elongated cylindrical masses, composed of irregularly wrapped layers; elongated cylindrical masses with constriction marks; and spirally-coiled specimens.

The function of prion-like protein Doppel was suggested to be related to male fertility. In this study, the importance of ovine Doppel polypeptide on spermatozoa capacitation and fertilization was evaluated. After refolding, recombinant Doppel (rDpl) was supplemented with different concentrations (40, 80 or 190 ng/ml) to ovine spermatozoa during the capacitation process. In experiment 1, post-thawed ovine spermatozoa were incubated with different concentrations of rDpl during 1 h for swim-up, and changes in sperm motility, concentration, vigour, viability and capacitation were monitored (10 replicates). In experiment 2, the fertilization ability of post-swim-up spermatozoa incubated as above was tested through heterologous fertilization of bovine in vitro matured oocytes (n = 423, three replicates). Regardless of dosage, rDpl improved (p = 0.03) spermatozoa viability. Sperm individual motility and vigour were the highest (p = 0.04) for the group receiving 190 ng/ml rDpl. Sperm supplemented with the highest doses of rDpl achieved higher (p = 0.02) fertilization rates (56.0 +/- 3.0%) than control (39.1 +/- 2.2%) and 40 ng/ml rDpl (39.8 +/- 2.7%). Preliminary data suggest that Doppel protein may enhance in vitro spermatozoa fertilizing ability.

Service orientation is a main trend in the development of support infrastructures for collaborative networks, namely in the manufacturing sector. Nevertheless, a literature review still shows the lack of a common understanding of the service concept, namely when we compare the software and business perspectives. As a contribution towards the integration of these two perspectives, this article introduces a conceptual framework based on the notion of representation of services in an ambassador-like manner through the introduction of a proactiveness mechanism. An example scenario and results with a prototype implementation are also discussed.

Collaborative-Networks (CN) have experienced a fast evolution in the last two decades. The
collaboration among independent entities or professionals supported by Information and
Communication Technology (ICT) has attracted the research community to establish the
conceptual basis for this scientific discipline. Service Orientation has been one of the key selected
paradigms for that conceptual basis. Nevertheless, the service concept itself does not have a
common understanding in the Business and ICT worlds. In the former, client satisfaction,
resources management and business process models are some example concerns, whilst the later
deals with interoperability, remote function calling or communication protocols.
If for example an enterprise provides some service, it may hire specialists to wrap such
service into web-services, expecting to reach worldwide potential new clients. In fact, nowadays
Web Services and Service Oriented Architectures (SOA) are the technological elements most
commonly used. However, these are passive elements in the sense they do not perform any action
towards pursuing business interests, which constitute a limiting factor from a business
perspective. Another approach for the above mentioned enterprise is to follow the Multi-Agent
Systems (MAS) approach, as the pro-activity is a keyword in such contexts. Nevertheless, as
MAS approaches are not so commonly used and not so robust yet, the worldwide potential set of
new clients is reduced; which also constitutes an inhibitor factor from the business perspective.
This dissertation proposes a Pro-Active Services Ecosystem Framework, gathering
inspiration from both the SOA and MAS research areas, trying to bridge the business and ICT
worlds through the base concepts for the creation of a Services’ Ecosystem where business
services are represented in a pro-active manner towards pursuing business interests, like finding
collaboration opportunities or improving the chances each CN member has to see its services
selected among competitors, for example. This work also includes a prototype system applied /
validated in the area of a Professional Virtual Community of Senior Professionals.

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The main objective of this paper is to propose a conceptual model about the influence of lean, agile, resilient, and green ({LARG)} practices on supply chain operational, economic and environmental performance. This model is based on the literature review about the four supply chain management paradigms and also performance measurement systems. This paper contributes to the literature by introducing a new model to improve the leanness, agility, resilience, and greenness of manufacturing supply chains. Among the suggested {LARG} practices, the ones influencing more the supply chain performance are the just in time and also the supplier relationships. Also the supply chain performance measures with more {LARG} practices influencing them are the inventory levels and the time, that is, the supply chain’s operational performance is the most affected by the simultaneous paradigms deployment in the supply chain.

This paper addresses an optimization-based approach for the design of RF integrated inductors. The methodology presented deals with the complexity of the design problem by formulating it as a multi-objective optimization. The multi-modal nature of the underlying functions combined with the need to be able to explore design trade-offs leads to the use of niching methods. This allows exploring not only the best trade-off solutions lying on the Pareto-optimum surface but also the quasi-optimum solutions that would be otherwise discarded. In this paper we take advantage of the niching properties of lbest PSO algorithm using ring topology to devise a simple optimizer able to find the local-optima. For the efficiency of the process analytical models are used for the passive/active devices. In spite the use of physics-based analytical expressions for the evaluation of the lumped elements, the variability of the process parameters is ignored in the optimization stage due to the significant computational burden it involves. Thus in the final stage both the Pareto-optimum solutions and the quasi-optimum solutions are evaluated with respect to the sensitivity to process parameter variations.

In this paper we present a quality model for spreadsheets, based on the ISO/IEC 9126 standard that defines a generic quality model for software. To each of the software characteristics defined in the ISO/IEC 9126, we associate an equivalent spreadsheet characteristic. Then, we propose a set of spreadsheet specific metrics to assess the quality of a spreadsheet in each of the defined characteristics. In order to obtain the normal distribution of expected values for a spreadsheet in each of the metrics that we propose, we have executed them against all spreadsheets in the large and widely used EUSES spreadsheet corpus. Then, we quantify each characteristic of our quality model after computing the values of our metrics, and we define quality scores for the different ranges of values. Finally, to automate the atribution of a quality score to a given spreadsheet, according to our quality model, we have integrated the computation of the metrics it includes in both a batch and a web-based tool.

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This tool demo paper presents SmellSheet Detective: a tool for automatically detecting bad smells in spreadsheets. We have defined a catalog of bad smells in spreadsheet data which was fully implemented in a reusable library for the manipulation of spreadsheets. This library is the building block of the SmellSheet Detective tool, that has been used to detect smells in large, real-world spreadsheet within the EUSES corpus, in order to validate and evolve our bad smells catalog.

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Native agars from Gracilaria vermiculophylla produced in sustainable aquaculture systems (IMTA) were extracted under conventional (TWE) and microwave (MAE) heating. The optimal extracts from both processes were compared in terms of their properties. The agars’ structure was further investigated through Fourier transform infrared and NMR spectroscopy. Both samples showed a regular structure with an identical backbone, β-D-galactose (G) and 3,6-anhydro-α-L-galactose (LA) units; a considerable degree of methylation was found at C6 of the G units and, to a lesser extent, at C2 of the LA residues. The methylation degree in the G units was lower for MAEopt agar; the sulfate content was also reduced. MAE led to higher agar recoveries with drastic extraction time and solvent volume reductions. Two times lower values of [η] and Mv obtained for the MAEopt sample indicate substantial depolymerization of the polysaccharide backbone; this was reflected in its gelling properties; yet it was clearly appropriate for commercial application in soft-texture food products.

The accumulation of lead in several bones of Wistar rats with time was determined and compared Q3 for the different types of bones. Two groups were studied: a control group (n = 20), not exposed to lead and a contaminated group (n = 30), exposed to lead from birth, first indirectly through
mother’s milk, and then directly through a diet containing lead acetate in drinking water (0.2%). Rats age ranged from 1 to 11 months, with approximately 1 month intervals and each of the collections had 3 contaminated rats and 2 control rats. Iliac, femur, tibia–fibula and skull have been analysed by energy dispersive X-ray fluorescence technique (EDXRF). Samples of formaldehyde used to preserve the bone tissues were also analysed by Electrothermal Atomic Absorption (ETAAS), showing that there was no significant loss of lead from the tissue to the preservative. The bones mean lead concentration of exposed rats range from 100 to 300 mg g 1 while control rats never exceeded 10 mg g 1. Mean bone lead concentrations were compared and
the concentrations were higher in iliac, femur and tibia–fibula and after that skull. However, of all the concentrations in the different collections, only those in the skull were statistically Q4 significantly different (p o 0.05) from the other types of bones. Analysis of a radar chart also allowed us to say that these differences tend to diminish with age. The Spearman correlation test applied to mean lead concentrations showed strong and very strong positive correlations between
all different types of bones. This test also showed that mean lead concentrations in bones are negatively correlated with the age of the animals. This correlation is strong in iliac and femur and very strong in tibia–fibula and skull. It was also shown that the decrease of lead accumulation with age is made by three plateaus of accumulation,

This work reports a novel strategy to prepare affinity composite membranes using supercritical fluid technology. By blending molecularly imprinted polymeric particles with PMMA, a porous hybrid structure with affinity to the template molecule, bisphenol A, was prepared using a supercritical carbon dioxide (scCO2)-assisted method. Membranes were characterized in terms of morphology, mechanical performance and transport properties. The ability of the polymers and hybrid membranes to adsorb bisphenol A was tested in aqueous solutions and fitted to a linearized Langmuir equation, showing that adsorption takes place at homogeneous affinity binding sites within the imprinted surface. Filtration experiments showed that the imprinted hybrid membrane was able to adsorb higher amounts of template even in non-equilibrium dynamic binding conditions. The hybridization of the PMMA membrane herein reported conveys two important improvements over neat PMMA membrane: it introduced molecular affinity towards the template molecule and significantly increased the permeability of the porous structures, which are key parameters in processes that involve membranes. This technique could expand the applications of polymeric beads powders and enhance the efficiency of the membrane's transport properties. Our work presents a new method to confer affinity to a porous structure by immobilization of imprinted polymers, combining polymer synthesis and membrane formation using supercritical fluid technology.

Molecularly imprinted polymeric particles with molecular recognition towards Bisphenol A (BPA) were synthesized for the first time using the semi-covalent imprinting approach in supercritical carbon dioxide (scCO2). The material{'}s affinity to BPA was achieved by co-polymerizing ethylene glycol dimethacrylate (EGDMA) with a template-containing monomer{,} Bisphenol A dimethacrylate (BPADM) in scCO2. Bisphenol A is then cleaved from the polymeric matrix by hydrolysis with tetrabutylammonium hydroxide (n-Bu4OH) also in a supercritical environment{,} taking advantage of the high diffusivity of scCO2. The selectivity of the molecular imprinted polymer (MIP) was assessed by evaluating its capability to bind BPA in comparison with progesterone and [small alpha]-ethinylestradiol. In addition{,} the cross-linked particles were used to prepare a PMMA-based hybrid imprinted membrane by a scCO2-assisted phase inversion method. Results show that the incorporation of MIP particles was able to confer molecular affinity to BPA to the membrane and that at dynamic conditions of filtration{,} this imprinted porous structure was able to adsorb a higher amount of BPA than the corresponding non-imprinted hybrid membrane. Our work represents a valuable greener alternative to conventional methods{,} for the synthesis of affinity materials which are able to maintain molecular recognition properties in water.

The C 1s and N 1s photoelectron spectra of gas-phase 5-aminotetrazole (5ATZ) were recorded using synchrotron radiation, with the aim of evaluating 1H/2H tautomer population ratios. The core-electron binding energies (CEBEs) were estimated from computational results, using the delta self-consistent-field (ΔSCF) approach. Simulated spectra were generated using these CEBEs and the results from Gaussian-n (Gn, n=1, 2 and 3) and Complete Basis Set (CBS-4M and CBS-Q) methods. Results reveal the almost exclusive predominance of the 2H-tautomer, with a 1H/2H ratio of ca. 0.12/0.88, taken from a gross analysis of the XPS C 1s spectrum, recorded at 365 K.

Spreadsheets are considered to be the most widely used programming language in the world, and reports have shown that 90% of real-world spreadsheets contain errors. In this work, we try to identify spreadsheet smells, a concept adapted from software, which consists of a surface indication that usually corresponds to a deeper problem. Our smells have been integrated in a tool, and were computed for a large spreadsheet repository. Finally, the analysis of the results we obtained led to the refinement of our initial catalog.