A novel type of catalytic copper cluster in nitrous oxide reductase

Brown, K., M. Tegoni, M. Prudencio, AS Pereira, S. Besson, J. J. Moura, I. Moura, and C. Cambillau. "A novel type of catalytic copper cluster in nitrous oxide reductase." Nature Structural Biology. 7 (2000): 191-195.


Nitrous oxide (N(2)O) is a greenhouse gas, the third most significant contributor to global warming. As a key process for N(2)O elimination from the biosphere, N(2)O reductases catalyze the two-electron reduction of N(2)O to N(2). These 2 x 65 kDa copper enzymes are thought to contain a CuA electron entry site, similar to that of cytochrome c oxidase, and a CuZ catalytic center. The copper anomalous signal was used to solve the crystal structure of N(2)O reductase from Pseudomonas nautica by multiwavelength anomalous dispersion, to a resolution of 2.4 Angstrom. The structure reveals that the CuZ center belongs to a new type of metal cluster, in which four copper ions are liganded by seven histidine residues. N(2)O binds to this center via a single copper ion. The remaining copper ions might act as an electron reservoir, assuring a fast electron transfer and avoiding the formation of dead-end products.


Times Cited: 166 Brown, K Tegoni, M Prudencio, M Pereira, AS Besson, S Moura, JJ Moura, I Cambillau, C

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