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2008
Correia, Cristina, Stephane Besson, Carlos D. Brondino, Pablo J. Gonzalez, Guy Fauque, Jorge Lampreia, Isabel Moura, and Jose J. G. Moura. "Biochemical and spectroscopic characterization of the membrane-bound nitrate reductase from Marinobacter hydrocarbonoclasticus 617." JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY. 13 (2008): 1321-1333. Abstract
Membrane-bound nitrate reductase from Marinobacter hydrocarbonoclasticus 617 can be solubilized in either of two ways that will ultimately determine the presence or absence of the small (I) subunit. The enzyme complex (NarGHI) is composed of three subunits with molecular masses of 130, 65, and 20 kDa. This enzyme contains approximately 14 Fe, 0.8 Mo, and 1.3 molybdopterin guanine dinucleotides per enzyme molecule. Curiously, one heme b and 0.4 heme c per enzyme molecule have been detected. These hemes were potentiometrically characterized by optical spectroscopy at pH 7.6 and two noninteracting species were identified with respective midpoint potentials at E(m) = + 197 mV (heme c) and-4.5 mV (heme b). Variable-temperature (4-120 K) X-band electron paramagnetic resonance (EPR) studies performed on both as-isolated and dithionite-reduced nitrate reductase showed, respectively, an EPR signal characteristic of a {[}3Fe-4S](+) cluster and overlapping signals associated with at least three types of {[}4Fe-4S](+) centers. EPR of the as-isolated enzyme shows two distinct pH-dependent Mo(V) signals with hyperfine coupling to a solvent-exchangeable proton. These signals, called ``lowpH'' and ``high-pH,'' changed to a pH-independent Mo(V) signal upon nitrate or nitrite addition. Nitrate addition to dithionite-reduced samples at pH 6 and 7.6 yields some of the EPR signals described above and a new rhombic signal that has no hyperfine structure. The relationship between the distinct EPR-active Mo(V) species and their plausible structures is discussed on the basis of the structural information available to date for closely related membrane-bound nitrate reductases.
2001
Correia, C., C. Carneiro, S. Besson, G. Fauque, J. Lampreia, I. Moura, and JJG Moura. "Spectroscopic characterization of the membrane nitrate reductase isolated from Pseudomonas nautica." JOURNAL OF INORGANIC BIOCHEMISTRY. 86 (2001): 186.
1996
Pereira, AS, R. Franco, MJ Feio, C. Pinto, J. Lampreia, MA Reis, J. Calvete, I. Moura, I. Beech, AR Lino, and JJG Moura. "Characterization of representative enzymes from a sulfate reducing bacterium implicated in the corrosion of steel." BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. 221 (1996): 414-421. Abstract
This communication reports the isolation, purification and characterization of key enzymes involved in dissimilatory sulfate reduction of a sulfate reducing bacterium classified as Desulfovibrio desulfuricans subspecies desulfuricans New Jersey (NCIMB 8313) (Ddd NJ). The chosen strain, originally recovered from a corroding cast iron heat exchanger, was grown in large scale batch cultures. Physico-chemical and spectroscopic studies of the purified enzymes were carried out. These analyses revealed a high degree of similarity between proteins isolated from the DddNJ strain and the homologous proteins obtained from Desulfomicrobium baculatus Norway 4. In view of the results obtained, taxonomic reclassification of Desulfovibrio desulfuricans subspecies desulfuricans New Jersey (NCIMB 8313) into Desulfomicrobium baculatus (New Jersey) is proposed. (C) 1996 Academic Press, Inc.
1991
Lampreia, J., G. Fauque, N. SPEICH, C. DAHL, I. Moura, HG TRUPER, and JJG Moura. "SPECTROSCOPIC STUDIES ON APS REDUCTASE ISOLATED FROM THE HYPERTHERMOPHILIC SULFATE-REDUCING ARCHAEBACTERIUM ARCHAEGLOBUS-FULGIDUS." BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. 181 (1991): 342-347.
1988
KREMER, DR, M. VEENHUIS, G. Fauque, HD PECK, J. LeGall, J. Lampreia, JJG Moura, and TA HANSEN. "IMMUNOCYTOCHEMICAL LOCALIZATION OF APS REDUCTASE AND BISULFITE REDUCTASE IN 3 DESULFOVIBRIO SPECIES." ARCHIVES OF MICROBIOLOGY. 150 (1988): 296-301.
1987
Lampreia, J., I. Moura, G. Fauque, AV XAVIER, J. LeGall, HD PECK, and JJG Moura. "ADENYLYL SULFATE (APS) REDUCTASE FROM DESULFOVIBRIO-GIGAS." RECUEIL DES TRAVAUX CHIMIQUES DES PAYS-BAS-JOURNAL OF THE ROYAL NETHERLANDS CHEMICAL SOCIETY. 106 (1987): 234.