Publications

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2025
Conca, Dario Valter, Fouzia Bano, Małgorzata Graul, Julius von Wirén, Lauriane Scherrer, Hudson Pace, Justas Svirelis, Konrad Thorsteinsson, Andreas Dahlin, and Marta Bally. "Variant-Specific Interactions at the Plasma Membrane: Heparan Sulfate’s Impact on SARS-CoV-2 Binding Kinetics." Analytical Chemistry (2025). AbstractWebsite
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Sharma, Himanshu, Marie N. Sorin, Kiran B. Sharma, and Lars-Anders Carlson. "Illuminating druggable dark matter in RNA virus replication using in situ cryo-EM." Current Opinion in Virology. 72 (2025): 101475. AbstractWebsite

Viral proteins typically exist in the context of complex virions or in the even more complex host cells in which they replicate. Hence, meaningful insights into virus protein structure often need to account for this context. Various flavors of in situ cryo-electron microscopy (cryo-EM), such as cryo-electron tomography, are key methods for the contextual study of virus protein structure in pleomorphic virions and host cells. Here, we review recent in situ cryo-EM work on three selected phenomena in RNA virus replication: the maturation and nuclear entry of HIV-1, the membrane-bound replication organelles of positive-sense RNA viruses, and the membrane-less viral factories of negative-sense RNA viruses. We highlight cases where the imaged phenomena are targets of novel antiviral drugs (such as the recently approved antiretroviral Lenacapavir), drug candidates, and antiviral strategies. Finally, we discuss recent technical advances that extend the reach of in situ cryo-EM in virology.

2024
Svedberg, Dennis, Rahel R. Winiger, Alexandra Berg, Himanshu Sharma, Christian Tellgren-Roth, Bettina A. Debrunner-Vossbrinck, Charles R. Vossbrinck, and Jonas Barandun. "Functional annotation of a divergent genome using sequence and structure-based similarity." BMC Genomics. 25 (2024). AbstractWebsite
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Sharma, Himanshu, Nathan Jespersen, Kai Ehrenbolger, Lars-Anders Carlson, and Jonas Barandun. "Ultrastructural insights into the microsporidian infection apparatus reveal the kinetics and morphological transitions of polar tube and cargo during host cell invasion." PLOS Biology. 22 (2024): e3002533. AbstractWebsite
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Shankar, Kasturika, Marie N. Sorin, Himanshu Sharma, Oskar Skoglund, Selma Dahmane, Josy Ter Beek, Solomon Tesfalidet, Louise Nenzén, and Lars-Anders Carlson. "In vitroreconstitution reveals membrane clustering and RNA recruitment by the enteroviral AAA+ ATPase 2C." PLOS Pathogens. 20(8): e10 (2024). AbstractWebsite
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2020
Ehrenbolger, Kai, Nathan Jespersen, Himanshu Sharma, Yuliya Y. Sokolova, Yuri S. Tokarev, Charles R. Vossbrinck, and Jonas Barandun. "Differences in structure and hibernation mechanism highlight diversification of the microsporidian ribosome." PLOS Biology. 18 (2020): e3000958. AbstractWebsite
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2019
Sharma, Himanshu, and B. Anand. "Ribosome assembly defects subvert initiation Factor3 mediated scrutiny of bona fide start signal." Nucleic Acids Research. 47 (2019): 11368-11386. AbstractWebsite
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2018
Punetha, Ankita, Payel Sarkar, Siddharth Nimkar, Himanshu Sharma, Yoganand KNR, and Siranjeevi Nagaraj. "Structural Bioinformatics: Life Through The 3D Glasses." 2018. Abstract
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2016
2013
Sharma, Himanshu, and Himanshu Sharma. "In-silico single nucleotide polymorphisms (SNP) mining of Sorghum bicolor genome." African Journal of Biotechnology (2013). Abstract
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