<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="6.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Thapper, A.</style></author><author><style face="normal" font="default" size="100%">Rizzi, A. C.</style></author><author><style face="normal" font="default" size="100%">Brondino, CD</style></author><author><style face="normal" font="default" size="100%">Wedd, A. G.</style></author><author><style face="normal" font="default" size="100%">Pais, R. J.</style></author><author><style face="normal" font="default" size="100%">Maiti, B. K.</style></author><author><style face="normal" font="default" size="100%">Moura, I</style></author><author><style face="normal" font="default" size="100%">Pauleta, S. R.</style></author><author><style face="normal" font="default" size="100%">Moura, J J</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Copper-substituted forms of the wild type and C42A variant of rubredoxin</style></title><secondary-title><style face="normal" font="default" size="100%">J Inorg Biochem</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Copper/*chemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">Genetic Variation</style></keyword><keyword><style  face="normal" font="default" size="100%">Molecular Structure</style></keyword><keyword><style  face="normal" font="default" size="100%">Rubredoxins/*chemistry/genetics</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2013</style></year><pub-dates><date><style  face="normal" font="default" size="100%">Oct</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/23829948</style></url></web-urls></urls><edition><style face="normal" font="default" size="100%">2013/07/09</style></edition><volume><style face="normal" font="default" size="100%">127</style></volume><pages><style face="normal" font="default" size="100%">232-7</style></pages><isbn><style face="normal" font="default" size="100%">1873-3344 (Electronic)0162-0134 (Linking)</style></isbn><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;In order to gain insights into the interplay between Cu(I) and Cu(II) in sulfur-rich protein environments, the first preparation and characterization of copper-substituted forms of the wild-type rubredoxin (Rd) from Desulfovibrio vulgaris Hildenborough are reported, as well as those of its variant C42A-Rd. The initial products appear to be tetrahedral Cu(I)(S-Cys)n species for the wild type (n=4) and the variant C42A (n=3, with an additional unidentified ligand). These species are unstable to aerial oxidation to products, whose properties are consistent with square planar Cu(II)(S-Cys)n species. These Cu(II) intermediates are susceptible to auto-reduction by ligand S-Cys to produce stable Cu(I) final products. The original Cu(I) center in the wild-type system can be regenerated by reduction, suggesting that the active site can accommodate Cu(I)(S-Cys)2 and Cys-S-S-Cys fragments in the final product. The absence of one S-Cys ligand prevents similar regeneration in the C42A-Rd system. These results emphasize the redox instability of Cu(II)-(S-Cys)n centers.&lt;/p&gt;
</style></abstract><accession-num><style face="normal" font="default" size="100%">23829948</style></accession-num><notes><style face="normal" font="default" size="100%">&lt;p&gt;Thapper, AndersRizzi, Alberto CBrondino, Carlos DWedd, Anthony GPais, Ricardo JMaiti, Biplab KMoura, IsabelPauleta, Sofia RMoura, Jose J GResearch Support, Non-U.S. Gov'tUnited StatesJournal of inorganic biochemistryJ Inorg Biochem. 2013 Oct;127:232-7. doi: 10.1016/j.jinorgbio.2013.06.003. Epub 2013 Jun 13.&lt;/p&gt;
</style></notes><auth-address><style face="normal" font="default" size="100%">Department of Photochemistry and Molecular Science, Uppsala University, P.O. Box 523, S-751 20 Uppsala, Sweden.</style></auth-address></record></records></xml>