@article {10113,
	title = {Copper-substituted forms of the wild type and C42A variant of rubredoxin},
	journal = {J Inorg Biochem},
	volume = {127},
	year = {2013},
	note = {<p>Thapper, AndersRizzi, Alberto CBrondino, Carlos DWedd, Anthony GPais, Ricardo JMaiti, Biplab KMoura, IsabelPauleta, Sofia RMoura, Jose J GResearch Support, Non-U.S. Gov{\textquoteright}tUnited StatesJournal of inorganic biochemistryJ Inorg Biochem. 2013 Oct;127:232-7. doi: 10.1016/j.jinorgbio.2013.06.003. Epub 2013 Jun 13.</p>
},
	month = {Oct},
	pages = {232-7},
	edition = {2013/07/09},
	abstract = {<p>In order to gain insights into the interplay between Cu(I) and Cu(II) in sulfur-rich protein environments, the first preparation and characterization of copper-substituted forms of the wild-type rubredoxin (Rd) from Desulfovibrio vulgaris Hildenborough are reported, as well as those of its variant C42A-Rd. The initial products appear to be tetrahedral Cu(I)(S-Cys)n species for the wild type (n=4) and the variant C42A (n=3, with an additional unidentified ligand). These species are unstable to aerial oxidation to products, whose properties are consistent with square planar Cu(II)(S-Cys)n species. These Cu(II) intermediates are susceptible to auto-reduction by ligand S-Cys to produce stable Cu(I) final products. The original Cu(I) center in the wild-type system can be regenerated by reduction, suggesting that the active site can accommodate Cu(I)(S-Cys)2 and Cys-S-S-Cys fragments in the final product. The absence of one S-Cys ligand prevents similar regeneration in the C42A-Rd system. These results emphasize the redox instability of Cu(II)-(S-Cys)n centers.</p>
},
	keywords = {Copper/*chemistry, Genetic Variation, Molecular Structure, Rubredoxins/*chemistry/genetics},
	isbn = {1873-3344 (Electronic)0162-0134 (Linking)},
	url = {http://www.ncbi.nlm.nih.gov/pubmed/23829948},
	author = {Thapper, A. and Rizzi, A. C. and Brondino, CD and Wedd, A. G. and Pais, R. J. and Maiti, B. K. and Moura, I and Pauleta, S. R. and Moura, J J}
}
