@article {10106,
	title = {Incorporation of molybdenum in rubredoxin: models for mononuclear molybdenum enzymes},
	journal = {J Biol Inorg Chem},
	volume = {20},
	number = {5},
	year = {2015},
	note = {<p>Maiti, Biplab KMaia, Luisa BSilveira, Celia MTodorovic, SmiljaCarreira, CintiaCarepo, Marta S PGrazina, RaquelMoura, IsabelPauleta, Sofia RMoura, Jose J GResearch Support, Non-U.S. Gov{\textquoteright}tGermanyJournal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic ChemistryJ Biol Inorg Chem. 2015 Jul;20(5):821-9. doi: 10.1007/s00775-015-1268-0. Epub 2015 May 7.</p>
},
	month = {Jul},
	pages = {821-9},
	edition = {2015/05/08},
	abstract = {<p>Molybdenum is found in the active site of enzymes usually coordinated by one or two pyranopterin molecules. Here, we mimic an enzyme with a mononuclear molybdenum-bis pyranopterin center by incorporating molybdenum in rubredoxin. In the molybdenum-substituted rubredoxin, the metal ion is coordinated by four sulfurs from conserved cysteine residues of the apo-rubredoxin and two other exogenous ligands, oxygen and thiol, forming a Mo((VI))-(S-Cys)4(O)(X) complex, where X represents -OH or -SR. The rubredoxin molybdenum center is stabilized in a Mo(VI) oxidation state, but can be reduced to Mo(IV) via Mo(V) by dithionite, being a suitable model for the spectroscopic properties of resting and reduced forms of molybdenum-bis pyranopterin-containing enzymes. Preliminary experiments indicate that the molybdenum site built in rubredoxin can promote oxo transfer reactions, as exemplified with the oxidation of arsenite to arsenate.</p>
},
	keywords = {Electrochemical Techniques, Electron Spin Resonance Spectroscopy, Molecular Structure, Molybdenum/chemistry/*metabolism, Oxidoreductases/chemistry/*metabolism, Rubredoxins/chemistry/*metabolism},
	isbn = {1432-1327 (Electronic)0949-8257 (Linking)},
	url = {http://www.ncbi.nlm.nih.gov/pubmed/25948393},
	author = {Maiti, B. K. and Maia, L. B. and Silveira, C. M. and Todorovic, S. and Carreira, C. and Carepo, M. S. and Grazina, R. and Moura, I and Pauleta, S. R. and Moura, J J}
}
