@article {Branquinho2011,
	title = {{Real-time monitoring of PCR amplification of proto-oncogene c-MYC using a Ta2O5 electrolyte-insulator-semiconductor sensor}},
	journal = {Biosensors and Bioelectronics},
	volume = {28},
	number = {1},
	year = {2011},
	note = {n/a},
	pages = {44{\textendash}49},
	publisher = {Elsevier B.V.},
	abstract = {<p>We present a new approach for real-time monitoring of PCR amplification of a specific sequence from the human c-MYC proto-oncogene using a Ta 2O 5 electrolyte-insulator-semiconductor (EIS) sensor. The response of the fabricated EIS sensor to cycle DNA amplification was evaluated and compared to standard SYBR-green fluorescence incorporation, showing it was possible to detect DNA concentration variations with 30mV/??M sensitivity. The sensor{\textquoteright}s response was then optimized to follow in real-time the PCR amplification of c-MYC sequence from a genomic DNA sample attaining an amplification profile comparable to that of a standard real-time PCR. Owing to the small size, ease of fabrication and low-cost, the developed Ta 2O 5 sensor may be incorporated onto a microfluidic device and then used for real-time PCR. Our approach may circumvent the practical and economical obstacles posed by current platforms that require an external fluorescence detector difficult to miniaturize and incorporate into a lab-on-chip system. ?? 2011 Elsevier B.V.</p>
},
	keywords = {Cancer, DNA quantification, EIS, Field effect based sensors, Real-time PCR, Sensors/Biosensors, Tantalum pentoxide},
	isbn = {1873-4235 (Electronic)$\backslash$r0956-5663 (Linking)},
	issn = {09565663},
	doi = {10.1016/j.bios.2011.06.039},
	url = {http://dx.doi.org/10.1016/j.bios.2011.06.039},
	author = {Branquinho, Rita and Veigas, Bruno and Pinto, Joana V. and Martins, Rodrigo and Fortunato, Elvira and Baptista, Pedro V.}
}
