@article {Veigas2014,
	title = {{Ion sensing (EIS) real-time quantitative monitorization of isothermal DNA amplification}},
	journal = {Biosensors and Bioelectronics},
	volume = {52},
	year = {2014},
	note = {n/a},
	pages = {50{\textendash}55},
	publisher = {Elsevier},
	abstract = {<p>Field-effect-based devices are becoming a basic structural element in a new generation of microbiosensors. Reliable molecular characterization of DNA and/or RNA is of paramount importance for disease diagnostics and to follow up alterations in gene expression profiles. The use of such devices for point-of-need diagnostics has been hindered by the need of standard or real-time PCR amplification procedures. The present work focuses on the development of a tantalum pentoxide (Ta2O5) based sensor for the real-time label free detection of DNA amplification via loop mediated isothermal amplification (LAMP) allowing for quantitative analysis of the cMYC proto-oncogene. The strategy based on the field effect sensor was tested within a range of 1??108-1011 copies of target DNA, and a linear relationship between the log copy number of the initial template DNA and threshold time was observed allowing for a semi-quantitative analysis of DNA template. The concept offers many of the advantages of isothermal quantitative real-time DNA amplification in a label free approach and may pave the way to point-of-care quantitative molecular analysis focused on ease of use and low cost. ?? 2013 Elsevier B.V.</p>
},
	keywords = {c-Myc, Cancer, DNA, EIS, Ion sensitive field effect sensors, Isothermal amplification, Label free, LAMP, qRT-PCR, Real time, Tantalum pentoxide},
	isbn = {1873-4235 (Electronic) 0956-5663 (Linking)},
	issn = {09565663},
	doi = {10.1016/j.bios.2013.08.029},
	url = {http://dx.doi.org/10.1016/j.bios.2013.08.029},
	author = {Veigas, Bruno and Branquinho, Rita and Pinto, Joana V. and Wojcik, Pawel J. and Martins, Rodrigo and Fortunato, Elvira and Baptista, Pedro V.}
}
