@article {Branquinho201144,
	title = {Real-time monitoring of PCR amplification of proto-oncogene c-MYC using a Ta2O5 electrolyte-insulator-semiconductor sensor},
	journal = {Biosensors and Bioelectronics},
	volume = {28},
	number = {1},
	year = {2011},
	note = {<p>cited By 8</p>
},
	pages = {44-49},
	abstract = {<p>We present a new approach for real-time monitoring of PCR amplification of a specific sequence from the human c-MYC proto-oncogene using a Ta 2O 5 electrolyte-insulator-semiconductor (EIS) sensor. The response of the fabricated EIS sensor to cycle DNA amplification was evaluated and compared to standard SYBR-green fluorescence incorporation, showing it was possible to detect DNA concentration variations with 30mV/μM sensitivity. The sensor{\textquoteright}s response was then optimized to follow in real-time the PCR amplification of c-MYC sequence from a genomic DNA sample attaining an amplification profile comparable to that of a standard real-time PCR. Owing to the small size, ease of fabrication and low-cost, the developed Ta 2O 5 sensor may be incorporated onto a microfluidic device and then used for real-time PCR. Our approach may circumvent the practical and economical obstacles posed by current platforms that require an external fluorescence detector difficult to miniaturize and incorporate into a lab-on-chip system. {\textcopyright} 2011 Elsevier B.V.</p>
},
	doi = {10.1016/j.bios.2011.06.039},
	url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-80052377197\&partnerID=40\&md5=15054a62c7e299130f0507574dd2403d},
	author = {Branquinho, R.a b , Veigas, B.c , Pinto, J.V.a , Martins, R.a , Fortunato, E.a , Baptista, P.V.c}
}
