@article {Murugesan2020, title = {Siglec-15 recognition of sialoglycans on tumor cell lines can occur independently of sialyl Tn antigen expression.}, journal = {Glycobiology}, year = {2020}, note = {

n/a

}, month = {may}, abstract = {

Siglec-15 is a conserved sialic acid-binding Ig-like lectin expressed on osteoclast progenitors that plays an important role in osteoclast development and function. It is also expressed by tumor-associated macrophages and by some tumors, where it is thought to contribute to the immunosuppressive microenvironment. It was shown previously that engagement of macrophage-expressed Siglec-15 with tumor cells expressing its ligand, sialyl Tn (sTn), triggered production of TGF-$\beta$. In the present study, we have further investigated the interaction between Siglec-15 and sTn on tumor cells and its functional consequences. Based on binding assays with lung and breast cancer cell lines and glycan-modified cells, we failed to see evidence for recognition of sTn by Siglec-15. However, using a microarray of diverse, structurally-defined glycans, we show that Siglec-15 binds with higher avidity to sialylated glycans other than sTn or related antigen sequences. In addition, we were unable to demonstrate enhanced TGF-$\beta$ secretion following co-culture of Siglec-15-expressing monocytic cells lines with tumor cells expressing sTn, or following Siglec-15 cross-linking with monoclonal antibodies. However, we did observe activation of the SYK/MAPK signaling pathway following antibody cross-linking of Siglec-15 that may modulate the functional activity of macrophages.

}, issn = {1460-2423 (Electronic)}, doi = {10.1093/glycob/cwaa048}, author = {Murugesan, Gavuthami and Correia, Viviana G. and Palma, Angelina S. and Chai, Wengang and Li, Chunxia and Feizi, Ten and Martin, Eva and Laux, Brigitte and Franz, Alexandra and Fuchs, Klaus and Weigle, Bernd and Crocker, Paul R} } @article {Ribeiro2020, title = {Molecular basis for the preferential recognition of beta 1,3-1,4-glucans by the family 11 carbohydrate-binding module from Clostridium~thermocellum.}, journal = {The FEBS journal}, volume = {287}, number = {13}, year = {2020}, note = {

n/a

}, month = {jul}, pages = {2723{\textendash}2743}, abstract = {

Understanding the specific molecular interactions between proteins and $\beta$1,3-1,4-mixed-linked d-glucans is fundamental to harvest the full biological and biotechnological potential of these carbohydrates and of proteins that specifically recognize them. The family 11 carbohydrate-binding module from Clostridium~thermocellum (CtCBM11) is known for its binding preference for $\beta$1,3-1,4-mixed-linked over $\beta$1,4-linked glucans. Despite the growing industrial interest of this protein for the biotransformation of lignocellulosic biomass, the molecular determinants of its ligand specificity are not well defined. In this report, a combined approach of methodologies was used to unravel, at a molecular level, the ligand recognition of CtCBM11. The analysis of the interaction by carbohydrate microarrays and NMR and the crystal structures of CtCBM11 bound to $\beta$1,3-1,4-linked glucose oligosaccharides showed that both the chain length and the position of the $\beta$1,3-linkage are important for recognition, and identified the tetrasaccharide Glc$\beta$1,4Glc$\beta$1,4Glc$\beta$1,3Glc sequence as a minimum epitope required for binding. The structural data, along with site-directed mutagenesis and ITC studies, demonstrated the specificity of CtCBM11 for the twisted conformation of $\beta$1,3-1,4-mixed-linked glucans. This is mediated by a conformation-selection mechanism of the ligand in the binding cleft through CH-$π$ stacking and a hydrogen bonding network, which is dependent not only on ligand chain length, but also on the presence of a $\beta$1,3-linkage at the reducing end and at specific positions along the $\beta$1,4-linked glucan chain. The understanding of the detailed mechanism by which CtCBM11 can distinguish between linear and mixed-linked $\beta$-glucans strengthens its exploitation for the design of new biomolecules with improved capabilities and applications in health and agriculture. DATABASE: Structural data are available in the Protein Data Bank under the accession codes 6R3M and 6R31.

}, issn = {1742-4658 (Electronic)}, doi = {10.1111/febs.15162}, author = {Ribeiro, Diana O. and Viegas, Aldino and Pires, Virg{\'\i}nia M R and Medeiros-Silva, Jo{\~a}o and Bule, Pedro and Chai, Wengang and Marcelo, Filipa and Fontes, Carlos M. G. A. and Cabrita, Eurico J. and Palma, Angelina S. and Carvalho, Ana Lu{\'\i}sa} } @article {Vendele2020, title = {{Mannan detecting C-type lectin receptor probes recognise immune epitopes with diverse chemical, spatial and phylogenetic heterogeneity in fungal cell walls.}}, journal = {PLoS pathogens}, volume = {16}, number = {1}, year = {2020}, note = {n/a}, month = {jan}, pages = {e1007927}, abstract = {

During the course of fungal infection, pathogen recognition by the innate immune system is critical to initiate efficient protective immune responses. The primary event that triggers immune responses is the binding of Pattern Recognition Receptors (PRRs), which are expressed at the surface of host immune cells, to Pathogen-Associated Molecular Patterns (PAMPs) located predominantly in the fungal cell wall. Most fungi have mannosylated PAMPs in their cell walls and these are recognized by a range of C-type lectin receptors (CTLs). However, the precise spatial distribution of the ligands that induce immune responses within the cell walls of fungi are not well defined. We used recombinant IgG Fc-CTLs fusions of three murine mannan detecting CTLs, including dectin-2, the mannose receptor (MR) carbohydrate recognition domains (CRDs) 4-7 (CRD4-7), and human DC-SIGN (hDC-SIGN) and of the $\beta$-1,3 glucan-binding lectin dectin-1 to map PRR ligands in the fungal cell wall of fungi grown in vitro in rich and minimal media. We show that epitopes of mannan-specific CTL receptors can be clustered or diffuse, superficial or buried in the inner cell wall. We demonstrate that PRR ligands do not correlate well with phylogenetic relationships between fungi, and that Fc-lectin binding discriminated between mannosides expressed on different cell morphologies of the same fungus. We also demonstrate CTL epitope differentiation during different phases of the growth cycle of Candida albicans and that MR and DC-SIGN labelled outer chain N-mannans whilst dectin-2 labelled core N-mannans displayed deeper in the cell wall. These immune receptor maps of fungal walls of in vitro grown cells therefore reveal remarkable spatial, temporal and chemical diversity, indicating that the triggering of immune recognition events originates from multiple physical origins at the fungal cell surface.

}, keywords = {analysis, C-Type, Cell Adhesion Molecules, Cell Surface, Cell Wall, chemistry, classification, Fungi, genetics, Humans, immunology, Lectins, Mannans, microbiology, Mycoses, phylogeny, receptors}, issn = {1553-7374 (Electronic)}, doi = {10.1371/journal.ppat.1007927}, author = {Vendele, Ingrida and Willment, Janet A. and Silva, Lisete M and Palma, Angelina S. and Chai, Wengang and Liu, Yan and Feizi, Ten and Spyrou, Maria and Stappers, Mark H T and Brown, Gordon D. and Gow, Neil A R} } @article {Pandeirada2019, title = {{Structural analysis and potential immunostimulatory activity of Nannochloropsis oculata polysaccharides.}}, journal = {Carbohydrate polymers}, volume = {222}, year = {2019}, note = {n/a}, month = {oct}, pages = {114962}, abstract = {

The relevance of microalgae biotechnology for producing high-value compounds with biomedical application, such as polysaccharides, has been increasing. Despite this, the knowledge about the composition and structure of microalgae polysaccharides is still scarce. In this work, water-soluble polysaccharides from Nannochloropsis oculata were extracted, fractionated, structurally analysed, and subsequently tested in terms of immunostimulatory activity. A combination of sugar and methylation analysis with interaction data of carbohydrate-binding proteins using carbohydrate microarrays disclosed the complex structural features of the different polysaccharides. These analyses showed that the water-soluble polysaccharides fractions from N. oculata were rich in ($\beta$1{\textrightarrow}3, $\beta$1{\textrightarrow}4)-glucans, ($\alpha$1{\textrightarrow}3)-, ($\alpha$1{\textrightarrow}4)-mannans, and anionic sulphated heterorhamnans. The immunostimulatory assay highlighted that these fractions could also stimulate murine B-lymphocytes. Thus, the N. oculata water-soluble polysaccharides show potential to be further explored for immune-mediated biomedical applications.

}, keywords = {analysis, Animals, B-Lymphocytes, chemistry, Deoxy Sugars, drug effects, Glucans, Immunization, immunology, Inbred BALB C, Mannans, Mice, Microalgae, pharmacology, Polysaccharides, Stramenopiles}, issn = {1879-1344 (Electronic)}, doi = {10.1016/j.carbpol.2019.06.001}, author = {Pandeirada, Carolina O and Maricato, {\'E}lia and Ferreira, S{\'o}nia S and Correia, Viviana G. and Pinheiro, Benedita A. and Evtuguin, Dmitry V and Palma, Angelina S. and Correia, Alexandra and Vilanova, Manuel and Coimbra, Manuel A and Nunes, Cl{\'a}udia} } @article {Gao2019, title = {{Chemoenzymatic Synthesis of O-Mannose Glycans Containing Sulfated or Nonsulfated HNK-1 Epitope.}}, journal = {Journal of the American Chemical Society}, volume = {141}, number = {49}, year = {2019}, note = {n/a}, month = {dec}, pages = {19351{\textendash}19359}, abstract = {

The human natural killer-1 (HNK-1) epitope is a unique sulfated trisaccharide sequence presented on O- and N-glycans of various glycoproteins and on glycolipids. It is overexpressed in the nervous system and plays crucial roles in nerve regeneration, synaptic plasticity, and neuronal diseases. However, the investigation of functional roles of HNK-1 in a more complex glycan context at the molecular level remains a big challenge due to lack of access to related structurally well-defined complex glycans. Herein, we describe a highly efficient chemoenzymatic approach for the first collective synthesis of HNK-1-bearing O-mannose glycans with different branching patterns, and for their nonsulfated counterparts. The successful strategy relies on both chemical glycosylation of a trisaccharide lactone donor for the introduction of sulfated HNK-1 branch and substrate promiscuities of bacterial glycosyltransferases that can tolerate sulfated substrates for enzymatic diversification. Glycan microarray analysis with the resulting complex synthetic glycans demonstrated their recognition by two HNK-1-specific antibodies including anti-HNK-1/N-CAM (CD57) and Cat-315, which provided further evidence for the recognition epitopes of these antibodies and the essential roles of the sulfate group for HNK-1 glycan-antibody recognition.

}, issn = {1520-5126 (Electronic)}, doi = {10.1021/jacs.9b08964}, author = {Gao, Tian and Yan, Jingyu and Liu, Chang-Cheng and Palma, Angelina S. and Guo, Zhimou and Xiao, Min and Chen, Xi and Liang, Xinmiao and Chai, Wengang and Cao, Hongzhi} } @article {Campanero-Rhodes2019, title = {{Microarray Strategies for Exploring Bacterial Surface Glycans and Their Interactions With Glycan-Binding Proteins.}}, journal = {Frontiers in microbiology}, volume = {10}, year = {2019}, note = {n/a}, pages = {2909}, abstract = {

Bacterial surfaces are decorated with distinct carbohydrate structures that may substantially differ among species and strains. These structures can be recognized by a variety of glycan-binding proteins, playing an important role in the bacteria cross-talk with the host and invading bacteriophages, and also in the formation of bacterial microcolonies and biofilms. In recent years, different microarray approaches for exploring bacterial surface glycans and their recognition by proteins have been developed. A main advantage of the microarray format is the inherent miniaturization of the method, which allows sensitive and high-throughput analyses with very small amounts of sample. Antibody and lectin microarrays have been used for examining bacterial glycosignatures, enabling bacteria identification and differentiation among strains. In addition, microarrays incorporating bacterial carbohydrate structures have served to evaluate their recognition by diverse host/phage/bacterial glycan-binding proteins, such as lectins, effectors of the immune system, or bacterial and phagic cell wall lysins, and to identify antigenic determinants for vaccine development. The list of samples printed in the arrays includes polysaccharides, lipopoly/lipooligosaccharides, (lipo)teichoic acids, and peptidoglycans, as well as sequence-defined oligosaccharide fragments. Moreover, microarrays of cell wall fragments and entire bacterial cells have been developed, which also allow to study bacterial glycosylation patterns. In this review, examples of the different microarray platforms and applications are presented with a view to give the current state-of-the-art and future prospects in this field.

}, issn = {1664-302X (Print)}, doi = {10.3389/fmicb.2019.02909}, author = {Campanero-Rhodes, Mar{\'\i}a Asunci{\'o}n and Palma, Angelina Sa and Men{\'e}ndez, Margarita and Sol{\'\i}s, Dolores} } @article {Rudkin2018, title = {Single human B cell-derived monoclonal anti-Candida antibodies enhance phagocytosis and protect against disseminated candidiasis.}, journal = {Nature communications}, volume = {9}, number = {1}, year = {2018}, note = {

n/a

}, month = {dec}, pages = {5288}, abstract = {

The high global burden of over one million annual lethal fungal infections reflects a lack of protective vaccines, late diagnosis and inadequate chemotherapy. Here, we have generated a unique set of fully human anti-Candida monoclonal antibodies (mAbs) with diagnostic and therapeutic potential by expressing recombinant antibodies from genes cloned from the B cells of patients suffering from candidiasis. Single class switched memory B cells isolated from donors serum-positive for anti-Candida IgG were differentiated in vitro and screened against recombinant Candida albicans Hyr1 cell wall protein and whole fungal cell wall preparations. Antibody genes from Candida-reactive B cell cultures were cloned and expressed in Expi293F human embryonic kidney cells to generate a panel of human recombinant anti-Candida mAbs that demonstrate morphology-specific, high avidity binding to the cell wall. The species-specific and pan-Candida mAbs generated through this technology display favourable properties for diagnostics, strong opsono-phagocytic activity of macrophages in vitro, and protection in a murine model of disseminated candidiasis.

}, keywords = {administration {\&} dosage, Animals, Antibodies, B-Lymphocytes, Candida, Candida albicans, Candidiasis, drug effects, Female, Fungal, genetics, Humans, immunology, Inbred BALB C, Mice, microbiology, Monoclonal, Phagocytosis, physiology, prevention {\&} control}, issn = {2041-1723 (Electronic)}, doi = {10.1038/s41467-018-07738-1}, author = {Rudkin, Fiona M and Raziunaite, Ingrida and Workman, Hillary and Essono, Sosthene and Belmonte, Rodrigo and MacCallum, Donna M and Johnson, Elizabeth M and Silva, Lisete M and Palma, Angelina S. and Feizi, Ten and Jensen, Allan and Erwig, Lars P and Gow, Neil A R} } @article {14908, title = {Insights Into Glucan Polysaccharide Recognition Using Glucooligosaccharide Microarrays With Oxime-Linked Neoglycolipid Probes}, journal = {Methods Enzymol.}, volume = {598}, year = {2018}, pages = {139-167}, author = {Liu, Y. and Palma, A. S. and Feizi, T. and Chai, W.} } @article {14909, title = {Novel Monoclonal Antibody L2A5 Specifically Targeting sialyl-Tn and Short Glycans Terminated by alpha-2-6 Sialic Acids}, journal = {Sci Rep.}, volume = {8}, year = {2018}, pages = {12196}, author = {Loureiro, LR and Sousa, DP and Ferreira, D and Chai, W. and Lima, L and Pereira, C and Lopes, CB and Correia, VG and Silva, LM and Li, C and Santos, LL and Ferreira, JA and Barbas, A and Palma, A. S. and Novo, C and Videira, PA} } @article {14907, title = {O-Glycome Beam Search Arrays for Carbohydrate Ligand Discovery}, journal = {Mol Cell Proteomics}, volume = {17}, year = {2018}, pages = {121-133}, author = {Li, Z and Gao, C and Zhang, Y and Palma, A. S. and et al} } @article {14906, title = {The minimum information required for a glycomics experiment (MIRAGE) project: improving the standards for reporting glycan microarray-based data.}, journal = {Glycobiology}, volume = {27}, year = {2017}, pages = {280-284}, author = {Liu Y and McBride R and Stoll M and Palma AS and et al} } @article {RN135, title = {Generation and characterization of β1,2-gluco-oligosaccharide probes from Brucella abortus cyclic β-glucan and their recognition by C-type lectins of the immune system}, journal = {Glycobiology}, year = {2016}, note = {n/a}, type = {Journal Article}, abstract = {n/a}, issn = {1460-2423}, doi = {10.1093/glycob/cww041}, url = {https://www.ncbi.nlm.nih.gov/pubmed/27053576}, author = {Zhang, H. and Palma, A. S. and Zhang, Y and Childs, R. A. and Liu, Y. and Mitchell, D. A. and Guidolin, L. S. and Weigel, W. and Mulloy, B. and Ciocchini, A. E. and Feizi, T. and Chai, W.} } @article {RN133, title = {Influence of carrier lipid composition on glycan recognition in NGL-based microarrays}, journal = {Glycobiology}, volume = {25}, number = {11}, year = {2015}, note = {n/a}, pages = {1260}, type = {Journal Article}, abstract = {n/a}, issn = {0959-6658;1460-2423}, author = {Silva, Lisete and Childs, Robert A. and Palma, Angelina S. and Chai, Wengang and Feizi, Ten and Liu, Yan} } @article {RN130, title = {Unravelling Glucan Recognition Systems by Glycome Microarrays Using the Designer Approach and Mass Spectrometry}, journal = {Molecular \& Cellular Proteomics}, volume = {14}, number = {4}, year = {2015}, note = {n/a}, pages = {974-988}, type = {Journal Article}, abstract = {n/a}, issn = {1535-9476;1535-9484}, doi = {10.1074/mcp.M115.048272}, author = {Palma, Angelina S. and Liu, Yan and Zhang, Hongtao and Zhang, Yibing and McCleary, Barry V. and Yu, Guangli and Huang, Qilin and Guidolin, Leticia S. and Ciocchini, Andres E. and Torosantucci, Antonella and Wang, Denong and Carvalho, Ana Luisa and Fontes, Carlos M. G. A. and Mulloy, Barbara and Childs, Robert A. and Feizi, Ten and Chai, Wengang} } @article {RN129, title = {Conformational analysis of the Streptococcus pneumoniae hyaluronate lyase and characterization of its hyaluronan-specific carbohydrate-binding module.}, journal = {J Biol Chem}, year = {2014}, note = {

n/a

}, type = {Journal Article}, abstract = {

n/a

}, doi = {10.1074/jbc.M114.578435} } @article {RN125, title = {Carbohydrate Sequence of the Prostate Cancer-associated Antigen F77 Assigned by a Mucin O-Glycome Designer Array}, journal = {Journal of Biological Chemistry}, volume = {289}, number = {23}, year = {2014}, note = {n/a}, pages = {16462-16477}, type = {Journal Article}, abstract = {n/a}, issn = {0021-9258;1083-351X}, doi = {10.1074/jbc.M114.558932}, author = {Gao, Chao and Liu, Yan and Zhang, Hongtao and Zhang, Yibing and Fukuda, Michiko N. and Palma, Angelina S. and Kozak, Radoslaw P. and Childs, Robert A. and Nonaka, Motohiro and Li, Zhen and Siegel, Don L. and Hanfland, Peter and Peehl, Donna M. and Chai, Wengang and Greene, Mark I. and Feizi, Ten} } @article {RN126, title = {The neoglycolipid (NGL)-based oligosaccharide microarray system poised to decipher the meta-glycome}, journal = {Current Opinion in Chemical Biology}, volume = {18}, year = {2014}, note = {n/a}, pages = {87-94}, type = {Journal Article}, abstract = {n/a}, issn = {1367-5931;1879-0402}, doi = {10.1016/j.cbpa.2014.01.007}, author = {Palma, Angelina S. and Feizi, Ten and Childs, Robert A. and Chai, Wengang and Liu, Yan} } @article {RN123, title = {Changes in the hemagglutinin of H5N1 viruses during human infection - Influence on receptor binding}, journal = {Virology}, volume = {447}, number = {1-2}, year = {2013}, note = {n/a}, pages = {326-337}, type = {Journal Article}, abstract = {n/a}, issn = {0042-6822}, doi = {10.1016/j.virol.2013.08.010}, author = {Crusat, Martin and Liu, Junfeng and Palma, Angelina S. and Childs, Robert A. and Liu, Yan and Wharton, Stephen A. and Lin, Yi Pu and Coombs, Peter J. and Martin, Stephen R. and Matrosovich, Mikhail and Chen, Zi and Stevens, David J. and Hien, Vo Minh and Thanh, Tran Tan and Nhu, Le Nguyen Truc and Nguyet, Lam Anh and Ha, Do Quang and van Doorn, H. Rogier and Hien, Tran Tinh and Conradt, Harald S. and Kiso, Makoto and Gamblin, Steve J. and Chai, Wengang and Skehel, John J. and Hay, Alan J. and Farrar, Jeremy and de Jong, Menno D. and Feizi, Ten} } @article {RN127, title = {The Structural Basis of the Recognition of Di-glucosylated N-glycans by the ER Lectin Malectin}, journal = {Glycobiology}, volume = {23}, number = {11}, year = {2013}, note = {n/a}, pages = {1368-1369}, type = {Journal Article}, abstract = {n/a}, issn = {0959-6658;1460-2423}, author = {Palma, Angelina S. and Pinheiro, Benedita and Liu, Yan and Takeda, Yoichi and Chai, Wengang and Ito, Yukishige and Romao, Maria Joao and Carvalho, Ana Luisa and Feizi, Ten} } @article {RN122, title = {A Structure-Guided Mutation in the Major Capsid Protein Retargets BK Polyomavirus.}, journal = {PLoS pathogens}, volume = {9}, number = {10}, year = {2013}, note = {n/a}, pages = {e1003688}, type = {Journal Article}, abstract = {n/a}, issn = {1553-7374}, doi = {10.1371/journal.ppat.1003688}, author = {Neu, Ursula and Allen, Stacy-Ann A and Blaum, Barbel S and Liu, Yan and Frank, Martin and Palma, Angelina S. and Stroh, Luisa J and Feizi, Ten and Peters, Thomas and Atwood, Walter J and Stehle, Thilo} } @article {RN124, title = {Structures of B-Lymphotropic Polyomavirus VP1 in Complex with Oligosaccharide Ligands.}, journal = {PLoS pathogens}, volume = {9}, number = {10}, year = {2013}, note = {n/a}, pages = {e1003714}, type = {Journal Article}, abstract = {n/a}, issn = {1553-7374}, doi = {10.1371/journal.ppat.1003714}, author = {Neu, Ursula and Khan, Zaigham Mahmood and Schuch, Benjamin and Palma, Angelina S. and Liu, Yan and Pawlita, Michael and Feizi, Ten and Stehle, Thilo} } @article {RN93, title = {The C-type Lectin Receptor CLECSF8 (CLEC4D) Is Expressed by Myeloid Cells and Triggers Cellular Activation through Syk Kinase}, journal = {Journal of Biological Chemistry}, volume = {287}, number = {31}, year = {2012}, note = {n/a}, pages = {25964-25974}, type = {Journal Article}, abstract = {n/a}, issn = {0021-9258}, doi = {10.1074/jbc.M112.384164}, author = {Graham, Lisa M. and Gupta, Vandana and Schafer, Georgia and Reid, Delyth M. and Kimberg, Matti and Dennehy, Kevin M. and Hornsell, William G. and Guler, Reto and Campanero-Rhodes, Maria A. and Palma, Angelina S. and Feizi, Ten and Kim, Stella K. and Sobieszczuk, Peter and Willment, Janet A. and Brown, Gordon D.} } @article {RN92, title = {Designer-oligosaccharide microarrays to decipher ligands in mammalian and prokaryotic glucan-recognition systems}, journal = {Glycobiology}, volume = {22}, number = {11}, year = {2012}, note = {n/a}, pages = {1612-1613}, type = {Journal Article}, abstract = {n/a}, issn = {0959-6658}, author = {Palma, Angelina S. and Liu, Yan and Zhang, Yibing and Zhang, Hongtao and Luis, Ana S. and Carvalho, Ana Luisa and Gilbert, Harry J. and Boraston, Alisdair and Fontes, Carlos M. G. A. and Chai, Wengang and Feizi, Ten} } @article {RN131, title = {Neoglycolipid-based "designer" oligosaccharide microarrays to define beta-glucan ligands for Dectin-1.}, journal = {Methods in molecular biology (Clifton, N.J.)}, volume = {808}, year = {2012}, note = {n/a}, pages = {337-59}, type = {Journal Article}, abstract = {n/a}, issn = {1940-6029}, doi = {10.1007/978-1-61779-373-8_23}, author = {Palma, Angelina S. and Zhang, Yibing and Childs, Robert A. and Campanero-Rhodes, Maria A. and Liu, Yan and Feizi, Ten and Chai, Wengang} } @article {RN132, title = {Neoglycolipid-based oligosaccharide microarray system: preparation of NGLs and their noncovalent immobilization on nitrocellulose-coated glass slides for microarray analyses.}, journal = {Methods in molecular biology (Clifton, N.J.)}, volume = {808}, year = {2012}, note = {n/a}, pages = {117-36}, type = {Journal Article}, abstract = {n/a}, issn = {1940-6029}, doi = {10.1007/978-1-61779-373-8_8}, author = {Liu, Yan and Childs, Robert A. and Palma, Angelina S. and Campanero-Rhodes, Maria A. and Stoll, Mark S and Chai, Wengang and Feizi, Ten} } @article {RN97, title = {Bacterial, Fungal, and Algal Lectins: Combatants in Tug of War against HIV}, journal = {Structure}, volume = {19}, number = {8}, year = {2011}, note = {n/a}, pages = {1035-1037}, type = {Journal Article}, abstract = {n/a}, issn = {0969-2126}, doi = {10.1016/j.str.2011.07.006}, author = {Feizi, Ten and Liu, Yan and Palma, Angelina S.} } @article {RN100, title = {The human epithelial carcinoma antigen recognized by monoclonal antibody AE3 is expressed on a sulfoglycolipid in addition to neoplastic mucins}, journal = {Biochemical and Biophysical Research Communications}, volume = {408}, number = {4}, year = {2011}, note = {n/a}, pages = {548-552}, type = {Journal Article}, abstract = {n/a}, issn = {0006-291X}, doi = {10.1016/j.bbrc.2011.04.055}, author = {Palma, Angelina S. and Liu, Yan and Childs, Robert A. and Herbert, Colin and Wang, Denong and Chai, Wengang and Feizi, Ten} } @article {RN99, title = {The interactions of calreticulin with immunoglobulin G and immunoglobulin Y}, journal = {Biochimica Et Biophysica Acta-Proteins and Proteomics}, volume = {1814}, number = {7}, year = {2011}, note = {n/a}, pages = {889-899}, type = {Journal Article}, abstract = {n/a}, issn = {1570-9639}, doi = {10.1016/j.bbapap.2011.03.015}, author = {Mollegaard, Karen Mai and Duus, Karen and Traeholt, Sofie Dietz and Thaysen-Andersen, Morten and Liu, Yan and Palma, Angelina S. and Feizi, Ten and Hansen, Paul R. and Hojrup, Peter and Houen, Gunnar} } @article {RN96, title = {Plant production of anti-beta-glucan antibodies for immunotherapy of fungal infections in humans}, journal = {Plant Biotechnology Journal}, volume = {9}, number = {7}, year = {2011}, note = {n/a}, pages = {776-787}, type = {Journal Article}, abstract = {n/a}, issn = {1467-7644}, doi = {10.1111/j.1467-7652.2010.00586.x}, author = {Capodicasa, Cristina and Chiani, Paola and Bromuro, Carla and De Bernardis, Flavia and Catellani, Marcello and Palma, Angelina S. and Liu, Yan and Feizi, Ten and Cassone, Antonio and Benvenuto, Eugenio and Torosantucci, Antonella} } @article {RN95, title = {The Role of Sialyl Glycan Recognition in Host Tissue Tropism of the Avian Parasite Eimeria tenella}, journal = {Plos Pathogens}, volume = {7}, number = {10}, year = {2011}, note = {n/a}, type = {Journal Article}, abstract = {n/a}, issn = {1553-7366}, doi = {10.1371/journal.ppat.1002296}, author = {Lai, Livia and Bumstead, Janene and Liu, Yan and Garnett, James and Campanero-Rhodes, Maria A. and Blake, Damer P. and Palma, Angelina S. and Chai, Wengang and Ferguson, David J. P. and Simpson, Peter and Feizi, Ten and Tomley, Fiona M. and Matthews, Stephen} } @article {RN94, title = {Structural flexibility and ligand-binding characteristics of the macrophage dengue virus receptor CLEC5A}, journal = {Immunology}, volume = {135}, year = {2011}, note = {n/a}, pages = {101}, type = {Journal Article}, abstract = {n/a}, issn = {0019-2805}, author = {Watson, A. A. and Lebedev, A. A. and Hall, B. A. and Fenton-May, A. E. and Vagin, A. A. and Dejnirattisai, W. and Felce, J. and Mongkolsapaya, J. and Palma, A. S. and Liu, Y. and Feizi, T. and Screaton, G. R. and Murshudov, G. N. and O{\textquoteright}Callaghan, C. A.} } @article {RN98, title = {Structural Flexibility of the Macrophage Dengue Virus Receptor CLEC5A IMPLICATIONS FOR LIGAND BINDING AND SIGNALING}, journal = {Journal of Biological Chemistry}, volume = {286}, number = {27}, year = {2011}, note = {n/a}, pages = {24208-24218}, type = {Journal Article}, abstract = {n/a}, issn = {0021-9258}, doi = {10.1074/jbc.M111.226142}, author = {Watson, Aleksandra A. and Lebedev, Andrey A. and Hall, Benjamin A. and Fenton-May, Angharad E. and Vagin, Alexei A. and Dejnirattisai, Wanwisa and Felce, James and Mongkolsapaya, Juthathip and Palma, Angelina S. and Liu, Yan and Feizi, Ten and Screaton, Gavin R. and Murshudov, Garib N. and O{\textquoteright}Callaghan, Christopher A.} } @article {RN102, title = {Altered Receptor Specificity and Cell Tropism of D222G Hemagglutinin Mutants Isolated from Fatal Cases of Pandemic A(H1N1) 2009 Influenza Virus}, journal = {Journal of Virology}, volume = {84}, number = {22}, year = {2010}, note = {n/a}, pages = {12069-12074}, type = {Journal Article}, abstract = {n/a}, issn = {0022-538X}, doi = {10.1128/JVI.01639-10}, author = {Liu, Yan and Childs, Robert A. and Matrosovich, Tatyana and Wharton, Stephen and Palma, Angelina S. and Chai, Wengang and Daniels, Rodney and Gregory, Victoria and Uhlendorff, Jennifer and Kiso, Makoto and Klenk, Hans-Dieter and Hay, Alan and Feizi, Ten and Matrosovich, Mikhail} } @article {RN106, title = {Members of a Novel Protein Family Containing Microneme Adhesive Repeat Domains Act as Sialic Acid-binding Lectins during Host Cell Invasion by Apicomplexan Parasites}, journal = {Journal of Biological Chemistry}, volume = {285}, number = {3}, year = {2010}, note = {n/a}, pages = {2064-2076}, type = {Journal Article}, abstract = {n/a}, issn = {0021-9258}, doi = {10.1074/jbc.M109.060988}, author = {Friedrich, Nikolas and Santos, Joana M. and Liu, Yan and Palma, Angelina S. and Leon, Ester and Saouros, Savvas and Kiso, Makoto and Blackman, Michael J. and Matthews, Stephen and Feizi, Ten and Soldati-Favre, Dominique} } @article {RN107, title = {MULTIFACETED APPROACHES INCLUDING NEOGLYCOLIPID OLIGOSACCHARIDE MICROARRAYS TO LIGAND DISCOVERY FOR MALECTIN}, journal = {Methods in Enzymology, Vol 478: Glycomics}, volume = {478}, year = {2010}, note = {n/a}, pages = {265-286}, type = {Journal Article}, abstract = {n/a}, issn = {0076-6879;978-0-12-381001-4}, doi = {10.1016/S0076-6879(10)78013-7}, author = {Palma, Angelina S. and Liu, Yan and Muhle-Goll, Claudia and Butters, Terry D. and Zhang, Yibing and Childs, Robert and Chai, Wengang and Feizi, Ten and Fukuda, M} } @article {RN104, title = {Polysaccharide mimicry of the epitope of the broadly neutralizing anti-HIV antibody, 2G12, induces enhanced antibody responses to self oligomannose glycans}, journal = {Glycobiology}, volume = {20}, number = {7}, year = {2010}, note = {n/a}, pages = {812-823}, type = {Journal Article}, abstract = {n/a}, issn = {0959-6658}, doi = {10.1093/glycob/cwq020}, author = {Dunlop, D. Cameron and Bonomelli, Camille and Mansab, Fatma and Vasiljevic, Snezana and Doores, Katie J. and Wormald, Mark R. and Palma, Angelina S. and Feizi, Ten and Harvey, David J. and Dwek, Raymond A. and Crispin, Max and Scanlan, Christopher N.} } @article {RN103, title = {Structure-Function Analysis of the Human JC Polyomavirus Establishes the LSTc Pentasaccharide as a Functional Receptor Motif}, journal = {Cell Host \& Microbe}, volume = {8}, number = {4}, year = {2010}, note = {n/a}, pages = {309-319}, type = {Journal Article}, abstract = {n/a}, issn = {1931-3128}, doi = {10.1016/j.chom.2010.09.004}, author = {Neu, Ursula and Maginnis, Melissa S. and Palma, Angelina S. and Stroeh, Luisa J. and Nelson, Christian D. S. and Feizi, Ten and Atwood, Walter J and Stehle, Thilo} } @article {RN110, title = {Carbohydrate microarrays: key developments in glycobiology}, journal = {Biological Chemistry}, volume = {390}, number = {7}, year = {2009}, note = {n/a}, pages = {647-656}, type = {Journal Article}, abstract = {n/a}, issn = {1431-6730}, doi = {10.1515/BC.2009.071}, author = {Liu, Yan and Palma, Angelina S. and Feizi, Ten} } @article {RN108, title = {The Latest on Malectin - A Lectin of the Endoplasmic Reticulum}, journal = {Glycobiology}, volume = {19}, number = {11}, year = {2009}, note = {n/a}, pages = {1301-1302}, type = {Journal Article}, abstract = {n/a}, issn = {0959-6658}, author = {Liu, Yan and Palma, Angelina S. and Chai, Wengang and Muhle-Goll, Claudia and Ito, Yukishige and Feizi, Ten} } @article {RN111, title = {Protection by Anti-beta-Glucan Antibodies Is Associated with Restricted beta-1,3 Glucan Binding Specificity and Inhibition of Fungal Growth and Adherence}, journal = {Plos One}, volume = {4}, number = {4}, year = {2009}, note = {n/a}, type = {Journal Article}, abstract = {n/a}, issn = {1932-6203}, doi = {10.1371/journal.pone.0005392}, author = {Torosantucci, Antonella and Chiani, Paola and Bromuro, Carla and De Bernardis, Flavia and Palma, Angelina S. and Liu, Yan and Mignogna, Giuseppina and Maras, Bruno and Colone, Marisa and Stringaro, Annarita and Zamboni, Silvia and Feizi, Ten and Cassone, Antonio} } @article {RN109, title = {Receptor-binding specificity of pandemic influenza A (H1N1) 2009 virus determined by carbohydrate microarray}, journal = {Nature Biotechnology}, volume = {27}, number = {9}, year = {2009}, note = {n/a}, pages = {797-799}, type = {Journal Article}, abstract = {n/a}, issn = {1087-0156}, doi = {10.1038/nbt0909-797}, author = {Childs, Robert A. and Palma, Angelina S. and Wharton, Steve and Matrosovich, Tatyana and Liu, Yan and Chai, Wengang and Campanero-Rhodes, Maria A. and Zhang, Yibing and Eickmann, Markus and Kiso, Makoto and Hay, Alan and Matrosovich, Mikhail and Feizi, Ten} } @article {RN114, title = {Establishment of a cell model of ALS disease: Golgi apparatus disruption occurs independently from apoptosis}, journal = {Biotechnology Letters}, volume = {30}, number = {4}, year = {2008}, note = {n/a}, pages = {603-610}, type = {Journal Article}, abstract = {n/a}, issn = {0141-5492}, doi = {10.1007/s10529-007-9595-z}, author = {Gomes, Catarina and Palma, Angelina S. and Almeida, Rui and Regalla, Manuela and McCluskey, Leo F. and Trojanowski, John Q. and Costa, Julia} } @article {RN112, title = {Malectin-a Novel Lectin of the Endoplasmic Reticulum and a Candidate New Player in the Early Steps Of Protein N-Glycosylation}, journal = {Glycobiology}, volume = {18}, number = {11}, year = {2008}, note = {n/a}, pages = {948-949}, type = {Journal Article}, abstract = {n/a}, issn = {0959-6658}, author = {Palma, Angelina S. and Liu, Yan and Campanero-Rhodes, Maria A. and Childs, Robert A. and Stoll, Mark S and Schallus, Thomas and Muhle-Goll, Claudia and Mackeen, Mukram and Chai, Wengang and Feizi, Ten} } @article {RN113, title = {Malectin: A novel carbohydrate-binding protein of the endoplasmic reticulum and a candidate player in the early steps of protein N-glycosylation}, journal = {Molecular Biology of the Cell}, volume = {19}, number = {8}, year = {2008}, note = {n/a}, pages = {3404-3414}, type = {Journal Article}, abstract = {n/a}, issn = {1059-1524}, doi = {10.1091/mbc.E08-04-0354}, author = {Schallus, Thomas and Jaeckh, Christine and Feher, Krisztina and Palma, Angelina S. and Liu, Yan and Simpson, Jeremy C. and Mackeen, Mukram and Stier, Gunter and Gibson, Toby J. and Feizi, Ten and Pieler, Tomas and Muhle-Goll, Claudia} } @article {RN115, title = {Proteomic analysis of plasma from Portuguese patients with familial amyotrophic lateral sclerosis}, journal = {Amyotrophic Lateral Sclerosis}, volume = {9}, number = {6}, year = {2008}, note = {n/a}, pages = {339-349}, type = {Journal Article}, abstract = {n/a}, issn = {1748-2968}, doi = {10.1080/17482960801934239}, author = {Palma, Angelina S. and De Carvalho, Mamede and Grammel, Nicolas and Pinto, Susana and Barata, Nuno and Conradt, Harald S. and Costa, Julia} } @article {RN116, title = {Ligands for the beta-glucan receptor, Dectin-1, assigned using "designer" microarrays of oligosaccharide probes (neoglycolipids) generated from glucan polysaccharides}, journal = {Journal of Biological Chemistry}, volume = {281}, number = {9}, year = {2006}, note = {n/a}, pages = {5771-5779}, type = {Journal Article}, abstract = {n/a}, issn = {0021-9258}, doi = {10.1074/jbc.M511461200}, author = {Palma, A. S. and Feizi, T. and Zhang, YB and Stoll, MS and Lawson, AM and Diaz-Rodriguez, E and Campanero-Rhodes, MA and Costa, J. and Gordon, S and Brown, GD and Chai, WG} } @article {RN121, title = {Biochemical characterization of plasma in amyotrophic lateral sclerosis: Amino acid and protein composition}, journal = {Amyotrophic Lateral Sclerosis and Other Motor Neuron Disorders}, volume = {6}, number = {2}, year = {2005}, note = {n/a}, pages = {104-110}, type = {Journal Article}, abstract = {n/a}, issn = {1466-0822}, doi = {10.1080/14660820410021320}, author = {Palma, A. and De Carvalho, M and Barata, N and Evangelista, T and Chicau, P. and REGALLA, M and Costa, J.} } @article {RN117, title = {Effect of the manganese ion on human alpha 3/4 fucosyltransferase III activity}, journal = {Biometals}, volume = {17}, number = {1}, year = {2004}, note = {n/a}, pages = {35-43}, type = {Journal Article}, abstract = {n/a}, issn = {0966-0844}, doi = {10.1023/A:1024485718843}, author = {Palma, A. S. and Morais, VA and Coelho, AV and Costa, J.} } @article {RN120, title = {Expression and characterization of recombinant human alpha-3/4-fucosyltransferase III from Spodoptera frugiperda (Sf9) and Trichoplusia ni (Tn) cells using the baculovirus expression system}, journal = {Biochemical Journal}, volume = {353}, year = {2001}, note = {n/a}, pages = {719-725}, type = {Journal Article}, abstract = {n/a}, issn = {0264-6021}, doi = {10.1042/0264-6021:3530719}, author = {Morais, VA and Serpa, J and Palma, A. S. and Costa, T. and Maranga, L and Costa, J.} } @article {RN118, title = {Localization, purification and specificity of the full-length membrane-bound form of human recombinant alpha 1,3/4-fucosyltransferase from BHK-21B cells}, journal = {Biochemical Journal}, volume = {357}, year = {2001}, note = {n/a}, pages = {803-810}, type = {Journal Article}, abstract = {n/a}, issn = {0264-6021}, doi = {10.1042/0264-6021:3570803}, author = {Sousa, VL and Costa, MT and Palma, A. S. and Enguita, F and Costa, J.} } @article {RN119, title = {A novel plant alpha 4-fucosyltransferase (Vaccinium myrtillus L.) synthesises the Lewis(a) adhesion determinant}, journal = {Febs Letters}, volume = {499}, number = {3}, year = {2001}, note = {n/a}, pages = {235-238}, type = {Journal Article}, abstract = {n/a}, issn = {0014-5793}, doi = {10.1016/S0014-5793(01)02568-6}, author = {Palma, A. S. and Vila-Verde, C and Pires, AS and Fevereiro, PS and Costa, J.} }