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Zhang, H., A. S. Palma, Y. Zhang, R. A. Childs, Y. Liu, D. A. Mitchell, L. S. Guidolin, W. Weigel, B. Mulloy, A. E. Ciocchini, T. Feizi, and W. Chai. "Generation and characterization of β1,2-gluco-oligosaccharide probes from Brucella abortus cyclic β-glucan and their recognition by C-type lectins of the immune system." Glycobiology (2016). AbstractWebsite
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Watson, A. A., A. A. Lebedev, B. A. Hall, A. E. Fenton-May, A. A. Vagin, W. Dejnirattisai, J. Felce, J. Mongkolsapaya, A. S. Palma, Y. Liu, T. Feizi, G. R. Screaton, G. N. Murshudov, and C. A. O'Callaghan. "Structural flexibility and ligand-binding characteristics of the macrophage dengue virus receptor CLEC5A." Immunology. 135 (2011): 101. Abstract
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Watson, Aleksandra A., Andrey A. Lebedev, Benjamin A. Hall, Angharad E. Fenton-May, Alexei A. Vagin, Wanwisa Dejnirattisai, James Felce, Juthathip Mongkolsapaya, Angelina S. Palma, Yan Liu, Ten Feizi, Gavin R. Screaton, Garib N. Murshudov, and Christopher A. O'Callaghan. "Structural Flexibility of the Macrophage Dengue Virus Receptor CLEC5A IMPLICATIONS FOR LIGAND BINDING AND SIGNALING." Journal of Biological Chemistry. 286 (2011): 24208-24218. Abstract
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Vendele, Ingrida, Janet A. Willment, Lisete M. Silva, Angelina S. Palma, Wengang Chai, Yan Liu, Ten Feizi, Maria Spyrou, Mark H. T. Stappers, Gordon D. Brown, and Neil A. R. Gow. "{Mannan detecting C-type lectin receptor probes recognise immune epitopes with diverse chemical, spatial and phylogenetic heterogeneity in fungal cell walls.}." PLoS pathogens. 16 (2020): e1007927. Abstract

During the course of fungal infection, pathogen recognition by the innate immune system is critical to initiate efficient protective immune responses. The primary event that triggers immune responses is the binding of Pattern Recognition Receptors (PRRs), which are expressed at the surface of host immune cells, to Pathogen-Associated Molecular Patterns (PAMPs) located predominantly in the fungal cell wall. Most fungi have mannosylated PAMPs in their cell walls and these are recognized by a range of C-type lectin receptors (CTLs). However, the precise spatial distribution of the ligands that induce immune responses within the cell walls of fungi are not well defined. We used recombinant IgG Fc-CTLs fusions of three murine mannan detecting CTLs, including dectin-2, the mannose receptor (MR) carbohydrate recognition domains (CRDs) 4-7 (CRD4-7), and human DC-SIGN (hDC-SIGN) and of the $\beta$-1,3 glucan-binding lectin dectin-1 to map PRR ligands in the fungal cell wall of fungi grown in vitro in rich and minimal media. We show that epitopes of mannan-specific CTL receptors can be clustered or diffuse, superficial or buried in the inner cell wall. We demonstrate that PRR ligands do not correlate well with phylogenetic relationships between fungi, and that Fc-lectin binding discriminated between mannosides expressed on different cell morphologies of the same fungus. We also demonstrate CTL epitope differentiation during different phases of the growth cycle of Candida albicans and that MR and DC-SIGN labelled outer chain N-mannans whilst dectin-2 labelled core N-mannans displayed deeper in the cell wall. These immune receptor maps of fungal walls of in vitro grown cells therefore reveal remarkable spatial, temporal and chemical diversity, indicating that the triggering of immune recognition events originates from multiple physical origins at the fungal cell surface.

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Torosantucci, Antonella, Paola Chiani, Carla Bromuro, Flavia De Bernardis, Angelina S. Palma, Yan Liu, Giuseppina Mignogna, Bruno Maras, Marisa Colone, Annarita Stringaro, Silvia Zamboni, Ten Feizi, and Antonio Cassone. "Protection by Anti-beta-Glucan Antibodies Is Associated with Restricted beta-1,3 Glucan Binding Specificity and Inhibition of Fungal Growth and Adherence." Plos One. 4 (2009). Abstract
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Sousa, VL, MT Costa, A. S. Palma, F. Enguita, and J. Costa. "Localization, purification and specificity of the full-length membrane-bound form of human recombinant alpha 1,3/4-fucosyltransferase from BHK-21B cells." Biochemical Journal. 357 (2001): 803-810. Abstract
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Silva, Lisete, Robert A. Childs, Angelina S. Palma, Wengang Chai, Ten Feizi, and Yan Liu. "Influence of carrier lipid composition on glycan recognition in NGL-based microarrays." Glycobiology. 25 (2015): 1260. Abstract
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Schallus, Thomas, Christine Jaeckh, Krisztina Feher, Angelina S. Palma, Yan Liu, Jeremy C. Simpson, Mukram Mackeen, Gunter Stier, Toby J. Gibson, Ten Feizi, Tomas Pieler, and Claudia Muhle-Goll. "Malectin: A novel carbohydrate-binding protein of the endoplasmic reticulum and a candidate player in the early steps of protein N-glycosylation." Molecular Biology of the Cell. 19 (2008): 3404-3414. Abstract
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Rudkin, Fiona M., Ingrida Raziunaite, Hillary Workman, Sosthene Essono, Rodrigo Belmonte, Donna M. MacCallum, Elizabeth M. Johnson, Lisete M. Silva, Angelina S. Palma, Ten Feizi, Allan Jensen, Lars P. Erwig, and Neil A. R. Gow. "Single human B cell-derived monoclonal anti-Candida antibodies enhance phagocytosis and protect against disseminated candidiasis." Nature communications. 9 (2018): 5288. Abstract

The high global burden of over one million annual lethal fungal infections reflects a lack of protective vaccines, late diagnosis and inadequate chemotherapy. Here, we have generated a unique set of fully human anti-Candida monoclonal antibodies (mAbs) with diagnostic and therapeutic potential by expressing recombinant antibodies from genes cloned from the B cells of patients suffering from candidiasis. Single class switched memory B cells isolated from donors serum-positive for anti-Candida IgG were differentiated in vitro and screened against recombinant Candida albicans Hyr1 cell wall protein and whole fungal cell wall preparations. Antibody genes from Candida-reactive B cell cultures were cloned and expressed in Expi293F human embryonic kidney cells to generate a panel of human recombinant anti-Candida mAbs that demonstrate morphology-specific, high avidity binding to the cell wall. The species-specific and pan-Candida mAbs generated through this technology display favourable properties for diagnostics, strong opsono-phagocytic activity of macrophages in vitro, and protection in a murine model of disseminated candidiasis.

Ribeiro, Diana O., Aldino Viegas, Virgínia M. R. Pires, João Medeiros-Silva, Pedro Bule, Wengang Chai, Filipa Marcelo, Carlos M. G. A. Fontes, Eurico J. Cabrita, Angelina S. Palma, and Ana Luísa Carvalho. "Molecular basis for the preferential recognition of beta 1,3-1,4-glucans by the family 11 carbohydrate-binding module from Clostridium thermocellum." The FEBS journal. 287 (2020): 2723-2743. Abstract

Understanding the specific molecular interactions between proteins and $\beta$1,3-1,4-mixed-linked d-glucans is fundamental to harvest the full biological and biotechnological potential of these carbohydrates and of proteins that specifically recognize them. The family 11 carbohydrate-binding module from Clostridium thermocellum (CtCBM11) is known for its binding preference for $\beta$1,3-1,4-mixed-linked over $\beta$1,4-linked glucans. Despite the growing industrial interest of this protein for the biotransformation of lignocellulosic biomass, the molecular determinants of its ligand specificity are not well defined. In this report, a combined approach of methodologies was used to unravel, at a molecular level, the ligand recognition of CtCBM11. The analysis of the interaction by carbohydrate microarrays and NMR and the crystal structures of CtCBM11 bound to $\beta$1,3-1,4-linked glucose oligosaccharides showed that both the chain length and the position of the $\beta$1,3-linkage are important for recognition, and identified the tetrasaccharide Glc$\beta$1,4Glc$\beta$1,4Glc$\beta$1,3Glc sequence as a minimum epitope required for binding. The structural data, along with site-directed mutagenesis and ITC studies, demonstrated the specificity of CtCBM11 for the twisted conformation of $\beta$1,3-1,4-mixed-linked glucans. This is mediated by a conformation-selection mechanism of the ligand in the binding cleft through CH-$π$ stacking and a hydrogen bonding network, which is dependent not only on ligand chain length, but also on the presence of a $\beta$1,3-linkage at the reducing end and at specific positions along the $\beta$1,4-linked glucan chain. The understanding of the detailed mechanism by which CtCBM11 can distinguish between linear and mixed-linked $\beta$-glucans strengthens its exploitation for the design of new biomolecules with improved capabilities and applications in health and agriculture. DATABASE: Structural data are available in the Protein Data Bank under the accession codes 6R3M and 6R31.

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Pandeirada, Carolina O., Élia Maricato, Sónia S. Ferreira, Viviana G. Correia, Benedita A. Pinheiro, Dmitry V. Evtuguin, Angelina S. Palma, Alexandra Correia, Manuel Vilanova, Manuel A. Coimbra, and Cláudia Nunes. "{Structural analysis and potential immunostimulatory activity of Nannochloropsis oculata polysaccharides.}." Carbohydrate polymers. 222 (2019): 114962. Abstract

The relevance of microalgae biotechnology for producing high-value compounds with biomedical application, such as polysaccharides, has been increasing. Despite this, the knowledge about the composition and structure of microalgae polysaccharides is still scarce. In this work, water-soluble polysaccharides from Nannochloropsis oculata were extracted, fractionated, structurally analysed, and subsequently tested in terms of immunostimulatory activity. A combination of sugar and methylation analysis with interaction data of carbohydrate-binding proteins using carbohydrate microarrays disclosed the complex structural features of the different polysaccharides. These analyses showed that the water-soluble polysaccharides fractions from N. oculata were rich in ($\beta$1→3, $\beta$1→4)-glucans, ($\alpha$1→3)-, ($\alpha$1→4)-mannans, and anionic sulphated heterorhamnans. The immunostimulatory assay highlighted that these fractions could also stimulate murine B-lymphocytes. Thus, the N. oculata water-soluble polysaccharides show potential to be further explored for immune-mediated biomedical applications.

Palma, A. S., VA Morais, AV Coelho, and J. Costa. "Effect of the manganese ion on human alpha 3/4 fucosyltransferase III activity." Biometals. 17 (2004): 35-43. Abstract
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Palma, Angelina S., Yan Liu, Maria A. Campanero-Rhodes, Robert A. Childs, Mark S. Stoll, Thomas Schallus, Claudia Muhle-Goll, Mukram Mackeen, Wengang Chai, and Ten Feizi. "Malectin-a Novel Lectin of the Endoplasmic Reticulum and a Candidate New Player in the Early Steps Of Protein N-Glycosylation." Glycobiology. 18 (2008): 948-949. Abstract
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Palma, Angelina S., Yibing Zhang, Robert A. Childs, Maria A. Campanero-Rhodes, Yan Liu, Ten Feizi, and Wengang Chai. "Neoglycolipid-based "designer" oligosaccharide microarrays to define beta-glucan ligands for Dectin-1." Methods in molecular biology (Clifton, N.J.). 808 (2012): 337-59. Abstract
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Palma, Angelina S., Benedita Pinheiro, Yan Liu, Yoichi Takeda, Wengang Chai, Yukishige Ito, Maria Joao Romao, Ana Luisa Carvalho, and Ten Feizi. "The Structural Basis of the Recognition of Di-glucosylated N-glycans by the ER Lectin Malectin." Glycobiology. 23 (2013): 1368-1369. Abstract
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Palma, A. S., C. Vila-Verde, AS Pires, PS Fevereiro, and J. Costa. "A novel plant alpha 4-fucosyltransferase (Vaccinium myrtillus L.) synthesises the Lewis(a) adhesion determinant." Febs Letters. 499 (2001): 235-238. Abstract
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Palma, Angelina S., Yan Liu, Robert A. Childs, Colin Herbert, Denong Wang, Wengang Chai, and Ten Feizi. "The human epithelial carcinoma antigen recognized by monoclonal antibody AE3 is expressed on a sulfoglycolipid in addition to neoplastic mucins." Biochemical and Biophysical Research Communications. 408 (2011): 548-552. Abstract
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Palma, Angelina S., Mamede De Carvalho, Nicolas Grammel, Susana Pinto, Nuno Barata, Harald S. Conradt, and Julia Costa. "Proteomic analysis of plasma from Portuguese patients with familial amyotrophic lateral sclerosis." Amyotrophic Lateral Sclerosis. 9 (2008): 339-349. Abstract
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Palma, A. S., T. Feizi, YB Zhang, MS Stoll, AM Lawson, E. Diaz-Rodriguez, MA Campanero-Rhodes, J. Costa, S. Gordon, GD Brown, and WG Chai. "Ligands for the beta-glucan receptor, Dectin-1, assigned using "designer" microarrays of oligosaccharide probes (neoglycolipids) generated from glucan polysaccharides." Journal of Biological Chemistry. 281 (2006): 5771-5779. Abstract
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Palma, Angelina S., Yan Liu, Claudia Muhle-Goll, Terry D. Butters, Yibing Zhang, Robert Childs, Wengang Chai, Ten Feizi, and M. Fukuda. "MULTIFACETED APPROACHES INCLUDING NEOGLYCOLIPID OLIGOSACCHARIDE MICROARRAYS TO LIGAND DISCOVERY FOR MALECTIN." Methods in Enzymology, Vol 478: Glycomics. 478 (2010): 265-286. Abstract
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Palma, Angelina S., Yan Liu, Hongtao Zhang, Yibing Zhang, Barry V. McCleary, Guangli Yu, Qilin Huang, Leticia S. Guidolin, Andres E. Ciocchini, Antonella Torosantucci, Denong Wang, Ana Luisa Carvalho, Carlos M. G. A. Fontes, Barbara Mulloy, Robert A. Childs, Ten Feizi, and Wengang Chai. "Unravelling Glucan Recognition Systems by Glycome Microarrays Using the Designer Approach and Mass Spectrometry." Molecular & Cellular Proteomics. 14 (2015): 974-988. Abstract
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Palma, Angelina S., Ten Feizi, Robert A. Childs, Wengang Chai, and Yan Liu. "The neoglycolipid (NGL)-based oligosaccharide microarray system poised to decipher the meta-glycome." Current Opinion in Chemical Biology. 18 (2014): 87-94. Abstract
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Palma, A., M. De Carvalho, N. Barata, T. Evangelista, P. Chicau, M. REGALLA, and J. Costa. "Biochemical characterization of plasma in amyotrophic lateral sclerosis: Amino acid and protein composition." Amyotrophic Lateral Sclerosis and Other Motor Neuron Disorders. 6 (2005): 104-110. Abstract
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Palma, Angelina S., Yan Liu, Yibing Zhang, Hongtao Zhang, Ana S. Luis, Ana Luisa Carvalho, Harry J. Gilbert, Alisdair Boraston, Carlos M. G. A. Fontes, Wengang Chai, and Ten Feizi. "Designer-oligosaccharide microarrays to decipher ligands in mammalian and prokaryotic glucan-recognition systems." Glycobiology. 22 (2012): 1612-1613. Abstract
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