@article {Rudkin2018,
	title = {Single human B cell-derived monoclonal anti-Candida antibodies enhance phagocytosis and protect against disseminated candidiasis.},
	journal = {Nature communications},
	volume = {9},
	number = {1},
	year = {2018},
	note = {<p>n/a</p>
},
	month = {dec},
	pages = {5288},
	abstract = {<p>The high global burden of over one million annual lethal fungal infections reflects a lack of protective vaccines, late diagnosis and inadequate chemotherapy. Here, we have generated a unique set of fully human anti-Candida monoclonal antibodies (mAbs) with diagnostic and therapeutic potential by expressing recombinant antibodies from genes cloned from the B cells of patients suffering from candidiasis. Single class switched memory B cells isolated from donors serum-positive for anti-Candida IgG were differentiated in vitro and screened against recombinant Candida albicans Hyr1 cell wall protein and whole fungal cell wall preparations. Antibody genes from Candida-reactive B cell cultures were cloned and expressed in Expi293F human embryonic kidney cells to generate a panel of human recombinant anti-Candida mAbs that demonstrate morphology-specific, high avidity binding to the cell wall. The species-specific and pan-Candida mAbs generated through this technology display favourable properties for diagnostics, strong opsono-phagocytic activity of macrophages in vitro, and protection in a murine model of disseminated candidiasis.</p>
},
	keywords = {administration {\&} dosage, Animals, Antibodies, B-Lymphocytes, Candida, Candida albicans, Candidiasis, drug effects, Female, Fungal, genetics, Humans, immunology, Inbred BALB C, Mice, microbiology, Monoclonal, Phagocytosis, physiology, prevention {\&} control},
	issn = {2041-1723 (Electronic)},
	doi = {10.1038/s41467-018-07738-1},
	author = {Rudkin, Fiona M and Raziunaite, Ingrida and Workman, Hillary and Essono, Sosthene and Belmonte, Rodrigo and MacCallum, Donna M and Johnson, Elizabeth M and Silva, Lisete M and Palma, Angelina S. and Feizi, Ten and Jensen, Allan and Erwig, Lars P and Gow, Neil A R}
}
