@article {Pandeirada2019,
	title = {{Structural analysis and potential immunostimulatory activity of Nannochloropsis oculata polysaccharides.}},
	journal = {Carbohydrate polymers},
	volume = {222},
	year = {2019},
	note = {n/a},
	month = {oct},
	pages = {114962},
	abstract = {<p>The relevance of microalgae biotechnology for producing high-value compounds with biomedical application, such as polysaccharides, has been increasing. Despite this, the knowledge about the composition and structure of microalgae polysaccharides is still scarce. In this work, water-soluble polysaccharides from Nannochloropsis oculata were extracted, fractionated, structurally analysed, and subsequently tested in terms of immunostimulatory activity. A combination of sugar and methylation analysis with interaction data of carbohydrate-binding proteins using carbohydrate microarrays disclosed the complex structural features of the different polysaccharides. These analyses showed that the water-soluble polysaccharides fractions from N. oculata were rich in ($\beta$1{\textrightarrow}3, $\beta$1{\textrightarrow}4)-glucans, ($\alpha$1{\textrightarrow}3)-, ($\alpha$1{\textrightarrow}4)-mannans, and anionic sulphated heterorhamnans. The immunostimulatory assay highlighted that these fractions could also stimulate murine B-lymphocytes. Thus, the N. oculata water-soluble polysaccharides show potential to be further explored for immune-mediated biomedical applications.</p>
},
	keywords = {analysis, Animals, B-Lymphocytes, chemistry, Deoxy Sugars, drug effects, Glucans, Immunization, immunology, Inbred BALB C, Mannans, Mice, Microalgae, pharmacology, Polysaccharides, Stramenopiles},
	issn = {1879-1344 (Electronic)},
	doi = {10.1016/j.carbpol.2019.06.001},
	author = {Pandeirada, Carolina O and Maricato, {\'E}lia and Ferreira, S{\'o}nia S and Correia, Viviana G. and Pinheiro, Benedita A. and Evtuguin, Dmitry V and Palma, Angelina S. and Correia, Alexandra and Vilanova, Manuel and Coimbra, Manuel A and Nunes, Cl{\'a}udia}
}
