@article { ISI:A1994NB53000038,
	title = {PRIMARY SEQUENCE, OXIDATION-REDUCTION POTENTIALS AND TERTIARY-STRUCTURE PREDICTION OF DESULFOVIBRIO-DESULFURICANS ATCC-27774 FLAVODOXIN},
	journal = {EUROPEAN JOURNAL OF BIOCHEMISTRY},
	volume = {220},
	number = {3},
	year = {1994},
	month = {MAR 15},
	pages = {987-995},
	abstract = {Flavodoxin was isolated and purified from Desulfovibrio desulfuricans ATCC 27774, a sulfatereducing organism that can also utilize nitrate as an alternative electron acceptor. Mid-point oxidation-reduction potentials of this flavodoxin were determined by ultraviolet/visible and EPR methods coupled to potentiometric measurements and their pH dependence studied in detail. The redox potential E(2), for the couple oxidized/semiquinone forms at pH 6.7 and 25 degrees C is -40 mV, while the value for the semiquinone/hydroquinone forms (E(1)), at the same pH, -387 mV. E(2) varies linearly with pH, while E(1) is independent of pH at high values. However, at low pH (<7.0), this value is less negative, compatible with a redox-linked protonation of the flavodoxin hydroquinone. A comparative study is presented for Desulfovibrio salexigens NCIB 8403 flavodoxin {[}Moura, I., Moura, J. J. G., Bruschi, M. and LeGall, J. (1980) Biochim. Biophys. Acta 591, 1-8]. The complete primary amino acid sequence was obtained by automated Edman degradation from peptides obtained by chemical and enzymic procedures. The amino acid sequence was confirmed by FAB/MS. Using the previously determined tridimensional structure of Desulfovibrio vulgaris flavodoxin as a model {[}similarity, 48,6\%; Watenpaugh, K. D., Sieker, L. C., Jensen, L. H., LeGall, J. and Dubourdieu M. (1972) Proc. Natl Acad. Sci. USA 69, 3185-3188], the tridimensional structure of D. desulfuricans ATCC 27774 flavodoxin was predicted using AMBER force-field calculations.},
	issn = {0014-2956},
	doi = {10.1111/j.1432-1033.1994.tb18703.x},
	author = {CALDEIRA, J and PALMA, PN and REGALLA, M and Lampreia, J and Calvete, J and SCHAFER, W and LeGall, J and Moura, I and Moura, JJG}
}
