Decomposição da amostra e estimação em difusões ergódicas - Luís Ramos (Faculdade de Ciências e Tecnologia da Universidade Nova de Lisboa, 2000)
The required results of stochastic calculus are introduced as well as sufficient conditions for a diffusin to be ergodic. Invariant densities are obtained for two families of diffusion. These families belong the Ornstein-Uhlenbeck and Cox-Ingersoll & Ross diffusions. The moments of transition density of the Cox-Ingersoll & Ross diffusion were obtained and it was shown that this density converges to the invariant density. Lastly a technique, based on sample partition, is given for parameter estimation for ergodic diffusion. A numerical application of that technique for the Ornstein-Uhlenbeck diffusion is given. Final remarks and comments are included.

Implémentation de comportements humains pour faciliter le guidage d’un robot mobile téléopéré - Y. Rybarczyk; Mestre D.; Colle E. (Journées des Jeunes Chercheurs en Robotique’00, 2000)

Involvement of Phaeoacremonium spp. and Cylindrocarpon destructans with grapevine decline in Portugal. - Rego, C.; Oliveira, H; Carvalho, A.; Phillips, AJL (Phytopathologia Mediterranea, 2000)

Punching of prestressed flat slabs: Experimental analysis - Ramos, A M P; Lúcio, V J G (International Workshop on Punching Shear Capacity of RC Flat Slabs, 2000)
The experimental analysis of nine prestressed flat slab models under punching is described and the results are compared with the recommendations of EC2 and MC90. The tests were performed on specimens at a scale 1/3, prestressed with unbonded high strength steel. In the first set, the specimens (AR2 to AR5 and AR7) were only subjected to in-plane compression, to evaluate the effect of the in-plane forces on the punching resistance. The second set of tests (AR8 to AR11) intended to study the effect of the vertical component of the tendon forces near the column in the punching resistance. This work aims to improve the understanding of the behaviour of prestressed flat slabs under punching load and the evaluation of the punching resistance.
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Repair and Strengthening Methods of Flat Slabs for Punching - Ramos, Antonio; Lucio, Valter; Regan, Paul E (International Workshop on Punching Shear Capacity of RC Flat Slabs, 2000)
The present work reports experimental research carried out on repair and strengthening methods of flat slabs for punching. The repair and strengthening methods studied are: strengthening using transversal prestress, repair by substitution of the damaged concrete; and strengthening using steel beams as a column head, connected to the column and to the slab with epoxy resin and mechanical expansion anchors. Four experimental test slabs (AR1 to AR4) were produced and tested: two with transversal prestress, one only repaired and one strengthened with a steel column head. The execution process, its efficiency and design, are discussed.
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Keywords: Flat slabs; Punching; repair; Strengthening

Reparação e Reforço do Viaduto do Fonte Nova - Ramos, A. M. P. e Lúcio, V (Encontro Nacional Betão Estrutural 2000, 2000)
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Application of Fuzzy Sets Theory to the Evaluation of Office Chairs - Isabel L. Nunes; Rita A. Ribeiro; Virgílio A. Machado (O Trabalho Humano com Sistemas Informatizados no Sector de Serviços, 2000)
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Flexibility, efficiency, and accountability: Adapting reserve selection algorithms to more complex conservation problems - Rodrigues, A.S.; Cerdeira, J.O.; Gaston, K.J. (Ecography, 2000)
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The Lupinus albus class-III chitinase gene, IF3, is constitutively expressed in vegetative organs and developing seeds - Regalado, AP; Pinheiro, C.; Vidal, S; Chaves, I; Ricardo, CPP; Rodrigues-Pousada, C (Planta, 2000)
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The Lupinus albus class-III chitinase gene, IF3, is constitutively expressed in vegetative organs and developing seeds - Regalado, AP; Pinheiro, C.; Vidal, S; Chaves, I; Ricardo, CPP; Rodrigues-Pousada, C (Planta, 2000)
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Pore dimension of water trees in PE: NMR studies - Judeinstein, P.; Lanca, M. C.; Marat-Mendes, J.; Rault, J. (Polymer, 2000)
In PE films aged under electric field the crystallisation of water (and melting of ice) has been studied by quadrupolar NMR, this technique allows one to determine the concentration of water as low as 10(-4). It is shown that the pore dimensions of the tracks forming the water trees of the order of 2.5 nm, are independent of the ageing time. The mobility of water in these water trees and in porous glass, of similar pore dimensions, are compared. (C) 2000 Elsevier Science Ltd. All rights reserved.
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Pore dimension of water trees in PE: NMR studies - Judeinstein, P.; Lanca, M. C.; Marat-Mendes, J.; Rault, J. (Polymer, 2000)
In PE films aged under electric field the crystallisation of water (and melting of ice) has been studied by quadrupolar NMR, this technique allows one to determine the concentration of water as low as 10(-4). It is shown that the pore dimensions of the tracks forming the water trees of the order of 2.5 nm, are independent of the ageing time. The mobility of water in these water trees and in porous glass, of similar pore dimensions, are compared. (C) 2000 Elsevier Science Ltd. All rights reserved.
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Preliminary results on UV and high temperature exposure effects on the electro-optical properties of cellulose derivatives based PDLC type cells - Almeida, PL; Cidade, M.T.; Godinho, M. H.; Ribeiro, AC; Figueirinhas, J. L. (Molecular Crystals and Liquid Crystals, 2000)
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Purification, characterization, and preliminary crystallographic study of copper-containing nitrous oxide reductase from Pseudomonas nautica 617 - Prudencio, M; Pereira, AS; Tavares, P.; Besson, S; Cabrito, I; Brown, K; Samyn, B; Devreese, B; VanBeeumen, J; Rusnak, F; Fauque, G; Moura, JJG; et al. (Biochemistry, 2000)
The aerobic purification of Pseudomonas nautica 617 nitrous oxide reductase yielded two forms of the enzyme exhibiting different chromatographic behaviors. The protein contains six copper atoms per monomer, arranged in two centers named CUA and Cut. Cut could be neither oxidized nor further reduced under our experimental conditions, and exhibits a 4-line EPR spectrum (g(x)= 2.015, A(x) = 1.5 mT, g(y) = 2.071, A(y) = 2 mT, g(z) = 2.138, A(z) = 7 mT) and a strong absorption at similar to 640 nm. Cu-A can be stabilized in a reduced EPR-silent state and in an oxidized state with a typical 7-line EPR spectrum (g(x) g(y) = 2.021, A(x) = A(y) = 0 T, g(z) =0.178, A(z) = 4 mT) and absorption bands at 480, 540, and similar to 800 nm. The difference between the two purified forms of nitrous oxide reductase is interpreted as a difference in the oxidation state of the CuA center. In form A, CUA is predominantly oxidized (S = 1/2, Cu1.5+-Cu1.5+), while in form B it is mostly in the one-electron reduced state (S = 0, Cu1+-Cu1+). In both forms, Cu-Z remains reduced (S = 1/2). Complete crystallographic data at 2.4 Angstrom indicate that Cu-A is a binuclear site (similar to the site found in cytochrome c oxidase) and Cu-Z is a novel tetracopper cluster [Brown, K., et ai. (2000) Nat. Struct. Biol. (in press)]. The complete amino acid sequence of the enzyme was determined and comparisons made with sequences of other nitrous oxide reductases, emphasizing the coordination of the centers. A 10.3 kDa peptide copurified with both forms of nitrous oxide reductase shows strong homology with proteins of the heat-shock GroES chaperonin family.
Times Cited: 52 Prudencio, M Pereira, AS Tavares, P Besson, S Cabrito, I Brown, K Samyn, B Devreese, B Van Beeumen, J Rusnak, F Fauque, G Moura, JJG Tegoni, M Cambillau, C Moura, I

Redox potential measurements of the Mycobacterium tuberculosis heme protein KatG and the isoniazid-resistant enzyme KatG(S315T): Insights into isoniazid activation - Wengenack, NL; Lopes, H; Kennedy, MJ; Tavares, P.; Pereira, AS; Moura, I; Moura, JJG; Rusnak, F (Biochemistry, 2000)
Mycobacterium tuberculosis KatG is a multifunctional heme enzyme responsible for activation of the antibiotic isoniazid. A KatG(S315T) point mutation is found in >50% of isoniazid-resistant clinical isolates. Since isoniazid activation is thought to involve an oxidation reaction, the redox potential of KatG was determined using cyclic voltammetry, square wave voltammetry, and spectroelectrochemical titrations. Isoniazid activation may proceed via a cytochrome P450-like mechanism. Therefore, the possibility that substrate binding by KatG leads to an increase in the heme redox potential and the possibility that KatG(S315T) confers isoniazid resistance by altering the redox potential were examined. Effects of the heme spin state on the reduction potentials of KatG and KatG(S315T) were also determined. Assessment of the Fe3+/Fe2+ couple gave a midpoint potential of ca. -50 mV for both KatG and KatG(S315T). In contrast to cytochrome P450s, addition of substrate had no significant effect on either the KatG or KatG(S315T) redox potential. Conversion of the heme to a low-spin configuration resulted in a -150 to -200 mV shift of the KatG and KatG(S315T) redox potentials. These results suggest that isoniazid resistance conferred by KatG(S315T) is not mediated through changes in the heme redox potential. The redox potentials of isoniazid were also determined using cyclic and square wave voltammetry, and the results provide evidence that the ferric KatG and KatG(S315T) midpoint potentials are too low to promote isoniazid oxidation without formation of a high-valent enzyme intermediate such as compounds I and IT or oxyferrous KatG.
Times Cited: 28 Wengenack, NL Lopes, H Kennedy, MJ Tavares, P Pereira, AS Moura, I Moura, JJG Rusnak, F

{Purification, characterization, and preliminary crystallographic study of copper-containing nitrous oxide reductase from Pseudomonas nautica 617} - Prudencio, M; Pereira, A.; Tavares, P.; Besson, S; Cabrito, I; Brown, K; Samyn, B; Devreese, B; VanBeeumen, J; Rusnak, F; Fauque, G; Moura, J; et al. (Biochemistry, 2000)

{Redox potential measurements of the Mycobacterium tuberculosis heme protein KatG and the isoniazid-resistant enzyme KatG(S315T): Insights into isoniazid activation} - Wengenack, NL; Lopes, H; Kennedy, MJ; Tavares, P.; Pereira, AS; Moura, I; Moura, JJG; Rusnak, F (Biochemistry, 2000)
Mycobacterium tuberculosis KatG is a multifunctional heme enzyme responsible for activation of the antibiotic isoniazid. A KatG(S315T) point mutation is found in >50% of isoniazid-resistant clinical isolates. Since isoniazid activation is thought to involve an oxidation reaction, the redox potential of KatG was determined using cyclic voltammetry, square wave voltammetry, and spectroelectrochemical titrations. Isoniazid activation may proceed via a cytochrome P450-like mechanism. Therefore, the possibility that substrate binding by KatG leads to an increase in the heme redox potential and the possibility that KatG(S315T) confers isoniazid resistance by altering the redox potential were examined. Effects of the heme spin state on the reduction potentials of KatG and KatG(S315T) were also determined. Assessment of the Fe3+/Fe2+ couple gave a midpoint potential of ca. -50 mV for both KatG and KatG(S315T). In contrast to cytochrome P450s, addition of substrate had no significant effect on either the KatG or KatG(S315T) redox potential. Conversion of the heme to a low-spin configuration resulted in a -150 to -200 mV shift of the KatG and KatG(S315T) redox potentials. These results suggest that isoniazid resistance conferred by KatG(S315T) is not mediated through changes in the heme redox potential. The redox potentials of isoniazid were also determined using cyclic and square wave voltammetry, and the results provide evidence that the ferric KatG and KatG(S315T) midpoint potentials are too low to promote isoniazid oxidation without formation of a high-valent enzyme intermediate such as compounds I and IT or oxyferrous KatG.

Un nouveau genre de dinosaure sauropode de la formation des Gres superieurs (Aptien-Albien) du Laos - Ronan Allain; Philippe Taquet; Bernard Battail; Jean Dejax; Philippe Richir; Monette Veran; Franck Limon-Duparcmeur; R. Vacant; O. Mateus; Phouvong Sayarath; Bounxou Khenthavong; Sitha Phouyavong (Comptes Rendus de l'Academie des Sciences - Series IIA - Earth and Planetary Science, 1999)
The partly-articulated postcranial remains of two sauropod skeletons recently found in Tang Vay (Savannakhet Province, Laos) are assigned to the species Tangvayosaurus hoffeti (nov. gen., nov. sp.). The derived characters present in the new material confirm the presence of titanosaurs in South East Asia at the end of the Early Cretaceous, but are not consistent with its placement within Titanosaurus genus as first done by Hoffet in 1942. All of the material relative to this species is therefore referred to a new genus: Tangvayosaurus. Tangvayosaurus and the Thai genus Phuwiangosaurus have strong affinities and are considered as primitive titanosaurs.
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Un nouveau genre de dinosaure sauropode de la formation des Grès supérieurs (Aptien-Albien) du Laos - Ronan Allain; Philippe Taquet; Bernard Battail; Jean Dejax; Philippe Richir; Monette Véran; Franck Limon-Duparcmeur; Renaud Vacant; Octavio Mateus; Phouvong Sayarath; Bounxou Khenthavong; Sitha Phouyavong (Comptes Rendus de l{\textquotesingle}Académie des Sciences - Series IIA - Earth and Planetary Science, 1999)
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Improving Distributed Intelligence in Complex Innovation Systems - Kuhlmann, Stefan; Boekholt, Patries; Georghiou, Luke; Guy, Ken; Heraud, Jean-Alain; Laredo, Philippe; Lemola, Tarmo; Loveridge, Denis; Luukkonen, Terttu; Moniz, António; Polt, Wolfgang; Ri (University Library of Munich, Germany, 1999)
Science and technology (S&T) are considered to be a central source, or at least a basic medium, of societal and industrial innovation, while innovation is conceived to basically feed the regeneration of our welfare. The suppliers of S&T in Europe as well as the users of their „products“, are confronted with a number of challenges today. We want to stress here that it was not the primary goal of our Advanced Science & Technology Policy Planning (ASTPP) Network to come up with proposals how the strategic character of European S&T policies could be strengthened. The ASTPP-network instead focuses on one aspect: the provision of strategic intelligence necessary to identify and develop strategic choices. The underlying hypothesis is that the existing body of experiences with technology foresight, technology assessment and S/T policy evaluation provides a basis for the development of an advanced S&T policy „planning“ approach by trying to enhance, interlink or even integrate the growing, but still dispersed experience in these three areas of intelligence. By „intelligent“ we mean that the inter-relatedness of S&T, industrial efforts, societal needs and political interventions becomes more transparent so that interactive collaboration between them will be facilitated.
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Keywords: assessment; evaluation; innovation; technology foresight; technology policy

Novel acid catalysed 1,4-addition-type ring-opening polymerisation of cyclic phosphorimidates - Cabrita, E. J.; Candeias, SX; Ramos, A. M.; Afonso, CAM; Santos, AG (Tetrahedron Letters, 1999)
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Integration of planning and control activities for building and construction: Experiencing standards - Jardim-Goncalves R; Sousa P; Pimentao JP; Steiger-Garcao A; Grilo A; Tadeu L (DURABILITY OF BUILDING MATERIALS AND COMPONENTS 8, VOLS 1-4, PROCEEDINGS, 1999)
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Abordagem à análise ergonómica de postos de trabalho através do sistema pericial difuso ERGO_X. [Workstation Ergonomic Analysis approach using the Fuzzy Expert System ERGO_X] - Isabel L. Nunes; Rita A. Ribeiro; Virgílio A. Machado (2º Conferência Internacional sobre Ergonomia, Segurança e Higiene Ocupacionais. [2nd International Conference on Ergonomics, Occupational Safety and Hygiene], 1999)
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Fuzzy evaluation module of ERGO_X - Isabel L. Nunes; Rita A. Ribeiro (Intelligent Systems and Active DSS - IFORS SPC9, 1999)
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ERGO_X - The evaluation process by means of a fuzzy multiple attribute decision making methodology - Isabel L. Nunes; Rita A. Ribeiro (International Conference: Computer-Aided Ergonomics and Safety - CAES'99, 1999)
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Biochemical and spectroscopic characterization of overexpressed fuscoredoxin from Escherichia coli - Pereira, AS; Tavares, P.; Krebs, C; HUYNH, BH; Rusnak, F; Moura, I; Moura, JJG (Biochemical and Biophysical Research Communications, 1999)
Fuscoredoxin is a unique iron containing protein of yet unknown function originally discovered in the sulfate reducers of the genus Desulfovibrio. It contains two iron-sulfur clusters: a cubane [4Fe-4S] and a mixed oxo- and sulfide-bridged 4Fe cluster of unprecedented structure. The recent determination of the genomic sequence of Escherichia coli (E. coli) has revealed a homologue of fuscoredoxin in this facultative microbe. The presence of this gene in E. coli raises interesting questions regarding the function of fuscoredoxin and whether this gene represents a structural homologue of the better-characterized Desulfovibrio proteins. In order to explore the latter, an overexpression system for the E. coli fuscoredoxin gene was devised. The gene was cloned from genomic DNA by use of the polymerase chain reaction into the expression vector pT7-7 and overexpressed in E. coli BL21(DE3) cells. After two chromatographic steps a good yield of recombinant protein was obtained (approximately 4 mg of pure protein per liter of culture). The purified protein exhibits an optical spectrum characteristic of the homologue from D. desulfuricans, indicating that cofactor assembly was accomplished. Iron analysis indicated that the protein contains circa 8 iron atoms/molecule which were shown by EPR and Mossbauer spectroscopies to be present as two multinuclear clusters, albeit with slightly altered spectroscopic features. A comparison of the primary sequences of fuscoredoxins is presented and differences on cluster coordination modes are discussed on the light of the spectroscopic data. (C) 1999 Academic Press.
Times Cited: 8 Pereira, AS Tavares, P Krebs, C Huynh, BH Rusnak, F Moura, I Moura, JJG

Cloning and overexpression of E.Coli fuscoredoxin - Pamplona, A; Pereira, AS; Tavares, P.; Moura, I; Rusnak, F; Moura, JJG (Journal of Inorganic Biochemistry, 1999)
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Times Cited: 0 Pamplona, A Pereira, AS Tavares, P Moura, I Rusnak, F Moura, JJG

III Relatório de Actividades do Protocolo Marinha Portuguesa / INESC - Goulão, Miguel; Monteiro, António Silva; Ribeiro, Nuno Palmeiro; Almeida, Alberto Bigotte; Abreu, Fernando Brito; Pedro Sousa (DAMAG / INESC, 1999)
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A new genus of sauropod dinosaur from the Gres superieurs formation (Aptian-Albian) of Laos - Allain, R.; Taquet, P; Battail, B.; Dejax, J.; Richir, P.; Veran, M.; Limon-Duparcmeur, F.; et al (Comptes Rendus de l'Academie des Sciences Series IIA Earth and Planetary Science, 1999)
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A new genus of sauropod dinosaur from the Gres superieurs formation (Aptian-Albian) of Laos | Un nouveau genre de dinosaure sauropode de la formation des Gres superieurs (Aptien-Albien) du Laos - Allain, R.; Taquet, P; Battail, B.; Dejax, J.; Richir, P.; Véran, M.; Limon-Duparcmeur, F.; et al (Comptes Rendus de l'Academie de Sciences - Serie IIa: Sciences de la Terre et des Planetes, 1999)
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Redox potential of the heme protein KatG from Mycobacterium tuberculosis - Wengenack, N; Lopes, H; Kennedy, M; Tavares, P.; Pereira, AS; Moura, I; Moura, JJG; Rusnak, F (Journal of Inorganic Biochemistry, 1999)
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Times Cited: 0 Wengenack, N Lopes, H Kennedy, M Tavares, P Pereira, AS Moura, I Moura, JJG Rusnak, F

SECTION IV: STRUCTURES-PHASE TRANSITIONS-A Small Angle Neutron Scattering Study of the Effect of Molecular Weight on the Conformation of Side Chain Liquid Crystal Polymers in a Smectic Phase - Richardson, RM; Barmatov, EB; Whitehouse, IJ; Shibaev, VP; Yongjie, T; Godinho, MHF (Molecular Crystals and Liquid Crystals, 1999)
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Sistemas periciais difusos em Ergonomia: O caso do ERGO_X - Isabel L. Nunes; Rita A. Ribeiro (Revista Portuguesa de Ergonomia, 1999)
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A small angle neutron scattering study of the effect of molecular weight on the conformation of side chain liquid crystal polymers in a smectic phase - Richardson, RM; Barmatov, EB; Whitehouse, IJ; Shibaev, VP; Yongjie, T; Godinho, MHF (Molecular Crystals and Liquid Crystals, 1999)
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Structural studies by X-ray diffraction on metal substituted desulforedoxin, a rubredoxin-type protein - Archer, M; Carvalho, AL; Teixeira, S; Moura, I; Moura, JJG; Rusnak, F; Romao, MJ (Protein Science, 1999)
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Un nouveau genre de dinosaure sauropode de la formation des Grès supérieurs (Aptien-Albien) du Laos - Allain, R.; Taquet, P; Battail, B.; Dejax, J.; Richir, P.; Véran, M.; Limon-Duparcmeur, F.; et al (Comptes Rendus de l'Académie des Sciences-Series IIA-Earth and Planetary Science, 1999)
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{Biochemical and spectroscopic characterization of overexpressed fuscoredoxin from Escherichia coli} - Pereira, AS; Tavares, P.; Krebs, C; HUYNH, BH; Rusnak, F; Moura, I; Moura, JJG (Biochemical And Biophysical Research Communications, 1999)
Fuscoredoxin is a unique iron containing protein of yet unknown function originally discovered in the sulfate reducers of the genus Desulfovibrio. It contains two iron-sulfur clusters: a cubane [4Fe-4S] and a mixed oxo- and sulfide-bridged 4Fe cluster of unprecedented structure. The recent determination of the genomic sequence of Escherichia coli (E. coli) has revealed a homologue of fuscoredoxin in this facultative microbe. The presence of this gene in E. coli raises interesting questions regarding the function of fuscoredoxin and whether this gene represents a structural homologue of the better-characterized Desulfovibrio proteins. In order to explore the latter, an overexpression system for the E. coli fuscoredoxin gene was devised. The gene was cloned from genomic DNA by use of the polymerase chain reaction into the expression vector pT7-7 and overexpressed in E. coli BL21(DE3) cells. After two chromatographic steps a good yield of recombinant protein was obtained (approximately 4 mg of pure protein per liter of culture). The purified protein exhibits an optical spectrum characteristic of the homologue from D. desulfuricans, indicating that cofactor assembly was accomplished. Iron analysis indicated that the protein contains circa 8 iron atoms/molecule which were shown by EPR and Mossbauer spectroscopies to be present as two multinuclear clusters, albeit with slightly altered spectroscopic features. A comparison of the primary sequences of fuscoredoxins is presented and differences on cluster coordination modes are discussed on the light of the spectroscopic data. (C) 1999 Academic Press.

{Cloning and overexpression of E.Coli fuscoredoxin} - Pamplona, A; Pereira, AS; Tavares, P.; Moura, I; Rusnak, F; Moura, JJG (Journal Of Inorganic Biochemistry, 1999)

{Redox potential of the heme protein KatG from Mycobacterium tuberculosis} - Wengenack, N; Lopes, H; Kennedy, M; Tavares, P.; Pereira, AS; Moura, I; Moura, JJG; Rusnak, F (Journal Of Inorganic Biochemistry, 1999)

Flexible molecules with defined shape. X. Synthesis and conformational study of 1,5-diaza-cis-decalin - Santos, AGD; W. Klute; J. Torode; V. P. W. Bohm; E. Cabrita; Runsink, J; Hoffmann, RW (New Journal of Chemistry, 1998)
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Electroinduced and spontaneous metal-halide bond dissociation in [Co(eta(5)-C5H5)(eta(3)-2-MeC3H4)I] - Teixeira, M. G.; Paolucci, F.; Marcaccio, M.; Aviles, T.; Paradisi, C.; Maran, F.; Roffia, S. (Organometallics, 1998)
The electrochemical behavior of the species [Co(eta(5)-C5H5)(eta(3)-2-MeC3H4)I] and [Co(eta(5)-C5H5)(eta(3)-2-MeC3H4)(ACN)](+) in ACN solutions, at 25 degrees C, is described. The kinetic analysis of the cyclic voltammetry curves indicates that the introduction of one electron in the former complex is concerted with the dissociation of the Co-I bond. The ensuing radical undergoes fast solvation to yield the solvato complex [Co(eta(5)-C5H5)(eta(3)-2-MeC3H4)(ACN)](.), which then acts as an efficient electron donor toward the starting material with the formation of[Co(eta(5)-C5H5)(eta(3)-2-MeC3H4)(ACN)](+); finally, the cation is electroreduced at the working potentials to conclude an overall autocatalytic sequence. The solvato complex [Co(eta(5)-C5H5)(eta(3)-2-MeC3H4)(ACN)](.), formed as a product of the above reduction process, can be reversibly reduced to the corresponding anion at more negative potentials. Confirmation of the above mechanism and of the fact that the solvato complex can act as a solution electron donor toward the starting material was obtained by studying the electrochemical behavior of the solvato complex itself and through calculations aimed to better define the dissociative electron-transfer process to [Co(eta(5)-C5H5)(eta(3)-2-MeC3H4)I]. The dissociation of the metal-halide bond in the neutral complex [Co(eta(5)-C5H5)(eta(3)-2-MeC3H4)I], with the formation of[Co(eta(5)-C5H5)(eta(3)-2-MeC3H4)(ACN)](+), was also found to occur spontaneously, in the bulk, through the observation of a progressive change of the cyclic voltammetric pattern. Support for the occurrence of the reaction between the starting complex and the solvent was confirmed by conductivity and spectroscopic measurements, which allowed the rate constant for the homogeneous solvolysis to be determined.
Zg590Times Cited:3Cited References Count:37
Keywords: breaking; Cations; cobalt; complexes; electron-transfer; Kinetics; potential sweep voltammetry; reduction; viii

ERGO_X - A fuzzy expert system for the design of workstations - Isabel L. Nunes; Rita A. Ribeiro; Virgílio A. Machado (First World Congress on Ergonomics for Global Quality and Productivity (ERGON-AXIA '98), 1998)
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ERGO_X - A system to deal with risk factors related with cumulative trauma disorders - Isabel L. Nunes; Rita A. Ribeiro; Virgílio A. Machado (II European Symposium of Ergonomics: Working Time Changes in Work and New Challenges, 1998)
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Phytochemical analysis and antimicrobial evaluation of Detarium microcarpum bark extracts - Abreu, P.M.; Rosa, V.S.; Araujo, E.M.; Canda, A.B.; Kayser, O.; Bindseil, K.-U.; Siems, K.; Seemann, A.; Seemann, A. (Pharmaceutical and Pharmacological Letters, 1998)
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Cited By :12Export Date: 17 June 2020

Theropod dinosaur nest from Lourinhã, Portugal - O. Mateus; Taquet, P; Antunes, MT; H Mateus; V Ribeiro (Journal of Vertebrate Paleontology, 1998)
More than 100 well preserved dinosaur eggs have been discovered in the Upper Jurassic levels (Tithonian) of Lourinhã, Portugal. The eggshels dispersed in a big area with 11 meters in the highest diameter with high concentration in the middle. [...............]
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Upper Jurassic Theropod Dinosaur embryos from Lourinhã (Portugal) - I Mateus; Mateus, Horácio; Antunes, MT; O. Mateus; Taquet, P; V Ribeiro; G Manuppella (Mem. Acad. Ciências de Lisboa, 1998)
Upper Jurassic nesting site from Paimogo (Lourinhã, Portugal) yielded the oldest dinosaur theropod embryos ever found. Numerous bones, including skull bones, from the skeleton of these embryos have been collected. The study of bones and embryos offers the possibility to learn more on the early life of theropod dinosaurs.
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Keywords: Eggs; Embryos; Portugal

Aplicação da Teoria dos Conjuntos Difusos em Ergonomia - Isabel L. Nunes; Rita A. Ribeiro; Virgílio A. Machado (Ingenium, 1998)
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II Relatório de Actividades do Protocolo Marinha Portuguesa / INESC - Goulão, Miguel; Monteiro, António Silva; Martins, José Furtado; Ribeiro, Nuno Palmeiro; Almeida, Alberto Bigotte; Abreu, Fernando Brito; Pedro Sousa (DAMAG / INESC, 1998)
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Spectroscopic characterization of a novel tetranuclear Fe cluster in an iron-sulfur protein isolated from Desulfovibrio desulfuricans - Tavares, P.; Pereira, AS; Krebs, C; Ravi, N; Moura, JJG; Moura, I; HUYNH, BH (Biochemistry, 1998)
Mossbauer and EPR spectroscopies were used to characterize the Fe clusters in an Fe-S protein isolated from Desulfovibrio desulfuricans (ATCC 27774). This protein was previously thought to contain hexanuclear Fe clusters, but a recent X-ray crystallographic measurement on a similar protein isolated from Desulfovibrio vulgaris showed that the protein contains two tetranuclear clusters, a cubane-type [4Fe-4S] cluster and a mixed-ligand cluster of novel structure [Lindley et al. (1997) Abstract, Chemistry of Metals in Biological Systems, European Research Conference, Tomar, Portugal]. Three protein samples poised at different redox potentials (as-purified, 40 and 320 mV) were investigated. In all three samples, the [4Fe-4S] cluster was found to be present in the diamagnetic 2+ oxidation state and exhibited typical Mossbauer spectra. The novel-structure cluster was found to be redox active. In the 320-mV and as-purified samples, the cluster is at a redox equilibrium between its fully oxidized and one-electron reduced states. In the 40-mV sample, the cluster is in a two-electron reduced state. Distinct spectral components associated with the four Fe sites of cluster 2 in the three oxidation states were identified. The spectroscopic parameters obtained for the Fe sites reflect different ligand environments, making it possible to assign the spectral components to individual Fe sites. In the fully oxidized state, all four iron ions are high-spin ferric and antiferromagnetically coupled to form a diamagnetic S = 0 state. In the one-electron and two-electron reduced states, the reducing electrons were found to localize, consecutively, onto two Fe sites that are rich in oxygen/nitrogen ligands. Based on the X-ray structure and the Mossbauer parameters, attempts could be made to identify the reduced Fe sites. For the two-electron reduced cluster, EPR and Mossbauer data indicate that the cluster is paramagnetic with a nonzero interger spin. For the one-electron reduced cluster, the data suggest a half-integer spin of 9/2 Characteristic fine and hyperfine parameters for all four Fe sites were obtained. Structural implications and the nature of the spin-coupling interactions are discussed.
Times Cited: 12 Tavares, P Pereira, AS Krebs, C Ravi, N Moura, JJG Moura, I Huynh, BH

Theropod dinosaur nest from Lourinhã, Portugal - O. Mateus; Taquet, P; Antunes, MT; H Mateus; V Ribeiro (Journal of Vertebrate Paleontology, 1998)
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Upper Jurassic Theropod Dinosaur embryos from Lourinhã (Portugal) - I Mateus; H Mateus; Antunes, MT; O. Mateus; Taquet, P; V Ribeiro; G Manuppella (Upper Jurassic paleoenvironments in Portugal, Mem. Acad. Ciências de Lisboa, 1998)
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{Spectroscopic characterization of a novel tetranuclear Fe cluster in an iron-sulfur protein isolated from Desulfovibrio desulfuricans} - Tavares, P.; Pereira, AS; Krebs, C; Ravi, N; Moura, JJG; Moura, I; HUYNH, BH (Biochemistry, 1998)
Mossbauer and EPR spectroscopies were used to characterize the Fe clusters in an Fe-S protein isolated from Desulfovibrio desulfuricans (ATCC 27774). This protein was previously thought to contain hexanuclear Fe clusters, but a recent X-ray crystallographic measurement on a similar protein isolated from Desulfovibrio vulgaris showed that the protein contains two tetranuclear clusters, a cubane-type [4Fe-4S] cluster and a mixed-ligand cluster of novel structure [Lindley et al. (1997) Abstract, Chemistry of Metals in Biological Systems, European Research Conference, Tomar, Portugal]. Three protein samples poised at different redox potentials (as-purified, 40 and 320 mV) were investigated. In all three samples, the [4Fe-4S] cluster was found to be present in the diamagnetic 2+ oxidation state and exhibited typical Mossbauer spectra. The novel-structure cluster was found to be redox active. In the 320-mV and as-purified samples, the cluster is at a redox equilibrium between its fully oxidized and one-electron reduced states. In the 40-mV sample, the cluster is in a two-electron reduced state. Distinct spectral components associated with the four Fe sites of cluster 2 in the three oxidation states were identified. The spectroscopic parameters obtained for the Fe sites reflect different ligand environments, making it possible to assign the spectral components to individual Fe sites. In the fully oxidized state, all four iron ions are high-spin ferric and antiferromagnetically coupled to form a diamagnetic S = 0 state. In the one-electron and two-electron reduced states, the reducing electrons were found to localize, consecutively, onto two Fe sites that are rich in oxygen/nitrogen ligands. Based on the X-ray structure and the Mossbauer parameters, attempts could be made to identify the reduced Fe sites. For the two-electron reduced cluster, EPR and Mossbauer data indicate that the cluster is paramagnetic with a nonzero interger spin. For the one-electron reduced cluster, the data suggest a half-integer spin of 9/2 Characteristic fine and hyperfine parameters for all four Fe sites were obtained. Structural implications and the nature of the spin-coupling interactions are discussed.

Couvée, oeufs et embryons d'un dinosaure théropode du Jurassique supérieur de Lourinhã (Portugal) - I Mateus; H Mateus; Antunes, MT; O. Mateus; Taquet, P; V Ribeiro; G Manuppella (C.R Acad. Sci. Paris, Sciences de la terre et des planetes, 1997)
Several well preserved clutches of dinosaurs have been discovered in the upper Kimmeridgian/ Tithonian of Lourinhã (Estramadur Province, Portugal). Some eggs of one clutch contained embryo elements of a theropod dinosaur. The egg-shell resembles that of eggs which have been discovered in the Upper Jurassic of Colorado

Myofibrillar and sarcoplasmic proteins and cathepsin D activity - Correia, JHRD; Lampreia, J; Matta, A; Mateus, C; Prates, J; Rosa, J; Correia, M; Correia, AD; Peixoto, C (FASEB JOURNAL, 1997)

Myofibrillar proteins and cathepsin D activity during different technologies and variations with time - Correia, AD; Correia, JHRD; Prates, J; Mateus, C; Matta, A; Rosa, J; Correia, M; Lampreia, J; Peixoto, C (FASEB JOURNAL, 1997)

Couvée, øe}ufs et embryons d{\textquotesingle}un Dinosaure Théropode du Jurassique supérieur de Lourinha (Portugal) - Isabel Mateus; Mateus, Horácio; Miguel Telles Antunes; Mateus,Octávio; Philippe Taquet; Vasco Ribeiro; Giuseppe Manuppella (Comptes Rendus de l{\textquotesingle}Académie des Sciences - Series IIA - Earth and Planetary Science, 1997)
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Ranking ergonomic office chairs via fuzzy sets - Isabel L. Nunes; Rita A. Ribeiro; Virgílio A. Machado (2º Congress International Franco-Quebecois- Le Genie Industriel dans un Monde sans Frontières, 1997)
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Acoustic determination of the critical surfaces in the ternary systems CO2 + CH2F2 + CF3CH2F and CO + C2H4 + CH3CHCH2 and in their binary subsystems - Kordikowski, A.; Robertson, D.G.; Poliakoff, M.; DiNoia, T.D.; McHugh, M.; Aguiar-Ricardo, A. (Journal of Physical Chemistry B, 1997)
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Cited By (since 1996):28Export Date: 19 November 2013Source: Scopus

Shear induced textures of thermotropic acetoxypropylcellulose - Riti, Jean-Bernard; Cidade, M.T.; Godinho, M. H.; Martins, AF; Navard, Patrick (SOR, 1997)
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Sistemas Periciais em Ergonomia - Isabel L. Nunes; Rita A. Ribeiro; Virgílio A. Machado (1ª Conferência Internacional sobre Ergonomia, Segurança e Higiene Ocupacionais, 1997)
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O tratamento da incerteza em Ergonomia via Conjuntos Difusos - Isabel L. Nunes; Rita A. Ribeiro; Virgílio A. Machado (1º Encontro Nacional de Engenharia e Gestão Industrial, 1997)
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Cascade control of a distributed collector solar field - Neves-Silva, R.; L. Rato; Lemos, J. M.; Coito, F. (Journal of Process Control, 1997)

Cascade control of a distributed collector solar field - Silva, RN; L. M. Rato; Lemos, J. M.; Coito, F. (Journal of Process Control, 1997)
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Couvée, oeufs et embryons d'un dinosaure théropode du Jurassique supérieur de Lourinhã (Portugal) - I Mateus; H Mateus; Antunes, MT; O. Mateus; Taquet, P; V Ribeiro; G Manuppella (Comptes Rendus de l'Académie des Sciences-Series IIA-Earth and Planetary Science, 1997)
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Couvée, œufs et embryons d'un Dinosaure Théropode du Jurassique supérieur de Lourinhã (Portugal) - I Mateus; H Mateus; Antunes, MT; O. Mateus; Taquet, P; V Ribeiro; G Manuppella (Comptes Rendus de l'Academie de Sciences - Serie IIa: Sciences de la Terre et des Planetes, 1997)
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Crystal structure of acidic seminal fluid protein (aSFP) at 1.9 angstrom resolution: a bovine polypeptide of the spermadhesin family - Romao, MJ; Kolln, I.; Dias, JM; Carvalho, AL; Romero, A.; Varela, P. F.; Sanz, L.; Topfer-Petersen, E.; Calvete, JJ (Journal of Molecular Biology, 1997)
We report the three-dimensional crystal structure of acidic seminal fluid protein (aSFP), a 12.9 kDa poly-peptide of the spermadhesin family isolated from bovine seminal plasma, solved by the multiple isomorphous replacement method and refined with data to 1.9 Angstrom resolution with a final R-factor of 17.3%. aSFP is built by a single CUB domain architecture, a 100 to 110 amino-acid-residue extracellular module found in 16 functionally diverse proteins. The structure of aSFP reveals that the CUB domain displays a beta-sandwich topology organised into two 5-stranded beta-sheets, each of which contain two parallel and four antiparallel strands. The structure of aSFP is almost identical to that of porcine spermadhesins PSP-I and PSP-II, which in turn show limited structural similarity with jellyroll topologies of certain virus capsid proteins. Essentially, topologically conserved residues in these proteins are those internal amino acids forming the hydrophobic core of the CUB and the jellyroll domains, suggesting their importance in maintaining the integrity of these protein folds, On the other hand, the structure of aSFP shows structural features that are unique to this protein and which may provide a structural ground for understanding the distinct biological properties of different members of the spermadhesin protein family. (C) 1997 Academic Press Limited.
ISI Document Delivery No.: YP198 Times Cited: 33 Cited Reference Count: 32 Romao, MJ Kolln, I Dias, JM Carvalho, AL Romero, A Varela, PF Sanz, L Topfer-Petersen, E Calvete, JJ ACADEMIC PRESS LTD LONDON
Keywords: bovine seminal plasma protein acidic seminal fluid protein aSFP spermadhesins CUB domain PELLUCIDA BINDING CHARACTERISTICS BOAR SPERMADHESINS PLASMA OLIGOSACCHARIDE LOCALIZATION LOCATION

The crystal structures of two spermadhesins reveal the CUB domain fold - Romero, A.; Romao, MJ; Varela, P. F.; Kolln, I.; Dias, JM; Carvalho, AL; Sanz, L.; TopferPetersen, E.; Calvete, JJ (Nature Structural Biology, 1997)
Spermadhesins, 12,000-14,000 M-r mammalian proteins, include lectins involved in sperm-egg binding and display a single CUB domain architecture. We report the crystal structures of porcine seminal plasma PSP-I/PSP-II, a heterodimer of two glycosylated spermadhesins. and bovine aSFP at 2.4 Angstrom and 1.9 Angstrom resolution respectively.
ISI Document Delivery No.: YA203 Times Cited: 108 Cited Reference Count: 32 Romero, A Romao, MJ Varela, PF Kolln, I Dias, JM Carvalho, AL Sanz, L TopferPetersen, E Calvete, JJ NATURE PUBLISHING CO NEW YORK
Keywords: SEMINAL FLUID PROTEIN BOAR SPERMADHESINS PSP-II PLASMA HETERODIMER LOCATION CLONING RNA

Crystallization and preliminary x-ray diffraction studies of aSFP, a bovine seminal plasma protein with a single CUB domain architecture - Dias, JM; Carvalho, AL; Kolln, I.; Calvete, JJ; TopferPetersen, E.; Varela, P. F.; Romero, A.; Urbanke, C.; Romao, MJ (Protein Science, 1997)
{Bovine acidic seminal fluid protein (aSFP) is a 12.9 kDa polypeptide of the spermadhesin family built by a single CUB domain architecture. The CUB domain is an extracellular module present in 16 functionally diverse proteins. To determine the three-dimensional structure of aSFP, the protein was crystallized at 21 degrees C by vapor diffusion in hanging drops, using ammonium sulfate, pH 4.7, and polyethyleneglycol 4000 as precipitants, containing 10% dioxane to avoid the formation of clustered crystals. Elongated prismatic crystals with maximal size of 0.6 x 0.3 x 0.2 mm(3) diffract to beyond 1.9 Angstrom resolution and belong to space group P2(1)2(1)2, with cell parameters a = 52.4 Angstrom
ISI Document Delivery No.: WM740 Times Cited: 7 Cited Reference Count: 22 Dias, JM Carvalho, AL Kolln, I Calvete, JJ TopferPetersen, E Varela, PF Romero, A Urbanke, C Romao, MJ CAMBRIDGE UNIV PRESS NEW YORK
Keywords: acidic seminal fluid protein analytical ultracentrifugation aSFP bovine seminal plasma crystallization CUB domain spermadhesin protein family X-ray diffraction analysis FLUID PROTEIN BOAR SPERMADHESINS ZONA-PELLUCIDA LOCALIZATION AWN-1

I Relatório de Actividades do Protocolo Marinha Portuguesa / INESC - Goulão, Miguel; Monteiro, António Silva; Ribeiro, Nuno Palmeiro; Almeida, Alberto Bigotte; Abreu, Fernando Brito; Pedro Sousa (DAMAG / INESC, 1997)
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Multirate MUSMAR cascade control of a distributed solar field - L. Rato; Silva, RN; Lemos, J. M.; Coito, F. (Proc. of the European Control Conference ECC97. Brussels, Belgium, 1997)
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Shear induced textures of thermotropic acetoxypropylcellulose - Riti, Jean-Bernard; Cidade, M.T.; Godinho, M. H.; Martins, AF; Navard, Patrick (Journal of Rheology, 1997)
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Uma introdução à aplicação da Teoria dos Conjuntos Difusos à Ergonomia - Isabel L. Nunes; Rita A. Ribeiro (I Congresso Nacional de Ergonomia, 1997)
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Characterization of representative enzymes from a sulfate reducing bacterium implicated in the corrosion of steel - Pereira, AS; Franco, R; Feio, MJ; Pinto, C; Lampreia, J; Reis, MA; Calvete, J; Moura, I; Beech, I; Lino, AR; Moura, JJG (BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1996)
This communication reports the isolation, purification and characterization of key enzymes involved in dissimilatory sulfate reduction of a sulfate reducing bacterium classified as Desulfovibrio desulfuricans subspecies desulfuricans New Jersey (NCIMB 8313) (Ddd NJ). The chosen strain, originally recovered from a corroding cast iron heat exchanger, was grown in large scale batch cultures. Physico-chemical and spectroscopic studies of the purified enzymes were carried out. These analyses revealed a high degree of similarity between proteins isolated from the DddNJ strain and the homologous proteins obtained from Desulfomicrobium baculatus Norway 4. In view of the results obtained, taxonomic reclassification of Desulfovibrio desulfuricans subspecies desulfuricans New Jersey (NCIMB 8313) into Desulfomicrobium baculatus (New Jersey) is proposed. (C) 1996 Academic Press, Inc.

Probing vapor/liquid equilibria of near-critical binary gas mixtures by acoustic measurements - Kordikowski, A.; Robertson, D.G.; Aguiar-Ricardo, A.I.; Popov, V.K.; Howdle, S.M.; Poliakoff, M. (Journal of Physical Chemistry, 1996)
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Cited By (since 1996):44Export Date: 19 November 2013Source: Scopus

Second virial coefficients of mixtures of xenon and lower hydrocarbons. 1. Experimental apparatus and results for Xe + C2He - Ricardo, A. A. (Journal of Physical Chemistry, 1996)
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Cited By (since 1996):9Export Date: 19 November 2013Source: Scopus

Second virial coefficients of mixtures of xenon and lower hydrocarbons. 2. Results for Xe + C2Ü4 and theoretical calculations - Ricardo, A. A. (Journal of Physical Chemistry, 1996)
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Cited By (since 1996):8Export Date: 19 November 2013Source: Scopus

A New Method for the Chiral HRGC Assay of L-2-Hydroxyglutaric Acid in Urine - H. J. Chaves das Neves; Noronha, J. P.; H. Rufino (J. High Resol. Chromatogr., 1996)

Reforço de Lajes Fungiformes ao Punçoamento com Perfis Metálicos - Ramos, A. M. P. e Lúcio, V (6º Encontro Nacional sobre Estruturas Pré-esforçadas, 1996)
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Characterization of representative enzymes from a sulfate reducing bacterium implicated in the corrosion of steel - Pereira, AS; Franco, R; Feio, MJ; Pinto, C; Lampreia, J; Reis, MA; Calvete, J; Moura, I; Beech, I; Lino, AR; Moura, JJG (Biochemical and Biophysical Research Communications, 1996)
This communication reports the isolation, purification and characterization of key enzymes involved in dissimilatory sulfate reduction of a sulfate reducing bacterium classified as Desulfovibrio desulfuricans subspecies desulfuricans New Jersey (NCIMB 8313) (Ddd NJ). The chosen strain, originally recovered from a corroding cast iron heat exchanger, was grown in large scale batch cultures. Physico-chemical and spectroscopic studies of the purified enzymes were carried out. These analyses revealed a high degree of similarity between proteins isolated from the DddNJ strain and the homologous proteins obtained from Desulfomicrobium baculatus Norway 4. In view of the results obtained, taxonomic reclassification of Desulfovibrio desulfuricans subspecies desulfuricans New Jersey (NCIMB 8313) into Desulfomicrobium baculatus (New Jersey) is proposed. (C) 1996 Academic Press, Inc.
Times Cited: 12 Pereira, AS Franco, R Feio, MJ Pinto, C Lampreia, J Reis, MA Calvete, J Moura, I Beech, I Lino, AR Moura, JJG

Em busca da Autonomia: Em torno dos movimentos sociais autonómicos dos Açores (1895-1975) - António B. Moniz; Gilberta Pavão Nunes Rocha (Congresso do 1º Centenário da Autonomia dos Açores, 1995)

Reparação e Reforço de Lajes Fungiformes ao Punçoamento - António Ramos (IST-UTL, 1995)
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Optical polarizing studies of cellulose acetate membranes prepared by phase-inversion - Rosa, MJ; M.N. de Pinho; Godinho, M. H.; Martins, AF (Molecular Crystals and Liquid Crystals, 1995)
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PRIMARY SEQUENCE, OXIDATION-REDUCTION POTENTIALS AND TERTIARY-STRUCTURE PREDICTION OF DESULFOVIBRIO-DESULFURICANS ATCC-27774 FLAVODOXIN - CALDEIRA, J; PALMA, PN; REGALLA, M; Lampreia, J; Calvete, J; SCHAFER, W; LeGall, J; Moura, I; Moura, JJG (EUROPEAN JOURNAL OF BIOCHEMISTRY, 1994)
Flavodoxin was isolated and purified from Desulfovibrio desulfuricans ATCC 27774, a sulfatereducing organism that can also utilize nitrate as an alternative electron acceptor. Mid-point oxidation-reduction potentials of this flavodoxin were determined by ultraviolet/visible and EPR methods coupled to potentiometric measurements and their pH dependence studied in detail. The redox potential E(2), for the couple oxidized/semiquinone forms at pH 6.7 and 25 degrees C is -40 mV, while the value for the semiquinone/hydroquinone forms (E(1)), at the same pH, -387 mV. E(2) varies linearly with pH, while E(1) is independent of pH at high values. However, at low pH (<7.0), this value is less negative, compatible with a redox-linked protonation of the flavodoxin hydroquinone. A comparative study is presented for Desulfovibrio salexigens NCIB 8403 flavodoxin {[}Moura, I., Moura, J. J. G., Bruschi, M. and LeGall, J. (1980) Biochim. Biophys. Acta 591, 1-8]. The complete primary amino acid sequence was obtained by automated Edman degradation from peptides obtained by chemical and enzymic procedures. The amino acid sequence was confirmed by FAB/MS. Using the previously determined tridimensional structure of Desulfovibrio vulgaris flavodoxin as a model {[}similarity, 48,6%; Watenpaugh, K. D., Sieker, L. C., Jensen, L. H., LeGall, J. and Dubourdieu M. (1972) Proc. Natl Acad. Sci. USA 69, 3185-3188], the tridimensional structure of D. desulfuricans ATCC 27774 flavodoxin was predicted using AMBER force-field calculations.

{Spectroscopic properties of desulfoferrodoxin from Desulfovibrio desulfuricans (ATCC 27774).} - Tavares, P.; Ravi, N; Moura, J J; LeGall, J; Huang, Y H; Crouse, B R; Johnson, M K; HUYNH, BH; Moura, I (Journal Of Biochemistry, 1994)
Desulfoferrodoxin, a non-heme iron protein, was purified previously from extracts of Desulfovibrio desulfuricans (ATCC 27774) (Moura, I., Tavares, P., Moura, J. J. G., Ravi, N., Huynh, B. H., Liu, M.-Y., and LeGall, J. (1990) J. Biol. Chem. 265, 21596-21602). The as-isolated protein displays a pink color (pink form) and contains two mononuclear iron sites in different oxidation states: a ferric site (center I) with a distorted tetrahedral sulfur coordination similar to that found in desulforedoxin from Desulfovibrio gigas and a ferrous site (center II) octahedrally coordinated with predominantly nitrogen/oxygen-containing ligands. A new form of desulfoferrodoxin which displays a gray color (gray form) has now been purified. Optical, electron paramagnetic resonance (EPR), and Mössbauer data of the gray desulfoferrodoxin indicate that both iron centers are in the high-spin ferric states. In addition to the EPR signals originating from center I at g = 7.7, 5.7, 4.1, and 1.8, the gray form of desulfoferrodoxin exhibits a signal at g = 4.3 and a shoulder at g = 9.6, indicating a high-spin ferric state with E/D approximately 1/3 for the oxidized center II. Redox titrations of the gray form of the protein monitored by optical spectroscopy indicate midpoint potentials of +4 +/- 10 and +240 +/- 10 mV for centers I and II, respectively. Mössbauer spectra of the gray form of the protein are consistent with the EPR finding that both centers are high-spin ferric and can be analyzed in terms of the EPR-determined spin Hamiltonian parameters. The Mössbauer parameters for both the ferric and ferrous forms of center II are indicative of a mononuclear high spin iron site with octahedral coordination and predominantly nitrogen/oxygen-containing ligands. Resonance Raman studies confirm the structural similarity of center I and the distorted tetrahedral FeS4 center in desulforedoxin and provide evidence for one or two cysteinyl-S ligands for center II. On the basis of the resonance Raman results, the 635 nm absorption band that is responsible for the gray color of the oxidized protein is assigned to a cysteinyl-S–>Fe(III) charge transfer transition localized on center II. The novel properties and possible function of center II are discussed in relation to those of mononuclear iron centers in other enzymes.
0021-9258 (Print) Journal Article

RHEOLOGY OF LIQUID-CRYSTALLINE CELLULOSE SOLUTIONS - RITI, JB; Cidade, M.T.; PATLAZHAN, S; Navard, P (AMER CHEMICAL SOC 1155 16TH ST, NW, WASHINGTON, DC 20036, 1994)
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Análise Experimental de Técnicas de Reparação e Reforço ao Punçoamento - Ramos, A. M. P. e Lúcio, V (5º Encontro Nacional sobre Estruturas Pré-esforçadas, 1994)
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Optical properties of cholesteric (2‐hydroxypropyl) cellulose (HPC) esters - Rusig, I; Godinho, M. H.; Varichon, L; Sixou, P; Dedier, J; Filliatre, C; Martins, AF (Journal of Polymer Science Part B: Polymer Physics, 1994)
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{Characterization of three proteins containing multiple iron sites: rubrerythrin, desulfoferrodoxin, and a protein containing a six-iron cluster} - Moura, I; Tavares, P.; Ravi, N (Methods Enzymol, 1994)
0076-6879 (Print) Journal Article

(p, Vm, T) of (0.476Ar + 0.524N2)(I) and the calculation of thermodynamic properties of liquid air - Ricardo, A. A.; Barreiros, S.F.; Nunes Da Ponte, M.; Albuquerque, G.M.N.; Calado, J.C.G. (The Journal of Chemical Thermodynamics, 1992)
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Cited By (since 1996):9Export Date: 19 November 2013Source: Scopus

Effect of Degradation on Thermotropic Cholesteric Optical Properties of (2-Hydroxypropyl)cellulose (HPC) Esters - Rusig, I; Dedier, J; Filliatre, C; Godinho, M. H.; Varichon, L; Sixou, P (Journal of Polymer Science: Part A: Polymer Chemistry, 1992)
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NMR study of molecular dynamics in a mixture of two polar liquid crystals (CBOOA and DOBCA) - Sebastião, PJ; Godinho, M. H.; Ribeiro, AC; Guillon, D; Vilfan, M (Liquid Crystals, 1992)
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{Purification and characterization of desulfoferrodoxin. A novel protein from Desulfovibrio desulfuricans (ATCC 27774) and from Desulfovibrio vulgaris (strain Hildenborough) that contains a distorted rubredoxin center and a mononuclear ferrous center} - Moura, I; Tavares, P.; Moura, J; Ravi, N; Huynh, B; Liu, M; LeGall, J (Journal Of Biological Chemistry, 1990)
A new type of non-heme iron protein was purified to homogeneity from extracts of Desulfovibrio desulfuricans (ATCC 27774) and Desulfovibrio vulgaris (strain Hildenborough). This protein is a monomer of 16-kDa containing two iron atoms per molecule. The visible spectrum has maxima at 495, 368, and 279 nm and the EPR spectrum of the native form shows resonances at g = 7.7, 5.7, 4.1 and 1.8 characteristic of a high-spin ferric ion (S = 5/2) with E/D = 0.08. Mossbauer data indicates the presence of two types of iron: an FeS4 site very similar to that found in desulforedoxin from Desulfovibrio gigas and an octahedral coordinated high-spin ferrous site most probably with nitrogen/oxygen-containing ligands. Due to this rather unusual combination of active centers, this novel protein is named desulfoferrodoxin. Based on NH2-terminal amino acid sequence determined so far, the desulfoferrodoxin isolated from D. desulfuricans (ATCC 27774) appears to be a close analogue to a recently discovered gene product from D. vulgaris (Brumlik, M.J., and Voordouw, G. (1989) J. Bacteriol. 171, 49996-50004), which was suggested to be a rubredoxin oxidoreductase. However, reduced pyridine nucleotides failed to reduce the desulforedoxin-like center of this new protein.
0021-9258 (Print) Journal Article

New Neutral and Cationic Cyclopentadienylcobalt Complexes - Aviles, T.; Barroso, F.; Royo, P. (Journal of Organometallic Chemistry, 1987)
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H8200Times Cited:2Cited References Count:12

New Eta-Allyl Eta-Cyclopentadienylcobalt Cations - Aviles, T.; Barroso, F.; Royo, P.; Noordik, J. H. (Journal of Organometallic Chemistry, 1982)
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Pj423Times Cited:7Cited References Count:9

Reactions of Monocyclopentadienyl-Cobalt Cations with Nucleophiles - Aviles, T.; Royo, P. (Journal of Organometallic Chemistry, 1981)
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Mu204Times Cited:5Cited References Count:5

Mono(Cyclopentadienyl)Molybdenum Chemistry - Hydrido-Derivatives, Halogeno-Derivatives, Allylic, and Butadiene Derivatives of [1,2-Bis(Diphenylphosphino)Ethane](Eta-Cyclopentadienyl)Molybdenum and Related Compounds - Aviles, T.; Green, M. L. H.; Dias, A. R.; Romao, C. (Journal of the Chemical Society-Dalton Transactions, 1979)
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Hm863Times Cited:41Cited References Count:11