@article {7410, title = {Synthesis and evaluation of new benzimidazole-based COX inhibitors: a naproxen-like interaction detected by STD-NMR}, journal = {RSC Advances}, volume = {5}, year = {2015}, pages = {49098-49109}, abstract = {

Non-steroidal anti-inflammatory drugs exert their pharmacological activity through inhibition of cyclooxygenase 1 and 2 (COX-1 and COX-2). Recent research suggests that a balanced inhibition of both COX-1 and COX-2 is the key to reduce the side-effects exhibited by COX inhibitors. We developed new benzimidazole-based compounds that showed a balanced COX inhibition, supported by molecular docking screening. The human whole blood assays demonstrated that the ester derivatives were potent inhibitors. Competitive saturation transfer difference (STD)-NMR experiments, in the presence of COX-2, using naproxen and diclofenac demonstrated that ester derivatives do not compete with diclofenac for the same binding site, but compete with the allosteric inhibitor naproxen. Combination of NMR spectroscopy with molecular docking has permitted us to detect a new naproxen-like inhibitor, which could be used for future drug development.

}, url = {http://pubs.rsc.org/en/content/articlelanding/2015/ra/c5ra04984a$\#$!divAbstract}, author = {L. C. R. Carvalho and Ribeiro, D and R. S. G. R. Seixas and A. M .S. Silva and M. Nave and A. C. Martins and S. Erhardt and Fernandes, E and Cabrita, E. J. and M. M. B. Marques} } @article {5838, title = {Assessing diffusion in enzyme loaded sol{\textendash}gel matrices}, journal = {RSC Advances}, volume = {4}, year = {2014}, pages = {25099-25105}, abstract = {

Pulsed field gradient spin echo high resolution magic angle spinning nuclear magnetic resonance spectroscopy is a powerful technique to characterize confined biosystems. We used this approach to assess the diffusion of solvent and reaction species within sol{\textendash}gel matrices differing in enzyme loading.

}, url = {http://pubs.rsc.org/en/content/articlelanding/2014/ra/c4ra01620c$\#$!divAbstract}, author = {Barreira, Gustavo and Ferreira, Ana S. D. and Pedro Vidinha and Cabral, Joaquim M. S. and Martinho, Jos{\'e} M. G. and Lima, Jo{\~a}o Carlos and Cabrita, Eurico J. and Barreiros, Susana} } @article {6484, title = {Epitope mapping of imidazolium cations in ionic liquid{\textendash}protein interactions unveils the balance between hydrophobicity and electrostatics towards protein destabilisation}, journal = {Phys. Chem. Chem. Phys. }, volume = {in press}, year = {2014}, abstract = {

We investigated imidazolium-based ionic liquid (IL) interactions with human serum albumin (HSA) to discern the level of cation interactions towards protein stability. STD-NMR spectroscopy was used to observe the imidazolium IL protons involved in direct binding and to identify the interactions responsible for changes in Tm as accessed by differential scanning calorimetry (DSC). Cations influence protein stability less than anions but still significantly. It was found that longer alkyl side chains of imidazolium- based ILs (more hydrophobic) are associated with a higher destabilisation effect on HSA than short-alkyl groups (less hydrophobic). The reason for such destabilisation lies on the increased surface contact area of the cation with the protein, particularly on the hydrophobic contacts promoted by the terminus of the alkyl chain. The relevance of the hydrophobic contacts is clearly demonstrated by the introduction of a polar moiety in the alkyl chain: a methoxy or alcohol group. Such structural modification reduces the degree of hydrophobic contacts with HSA explaining the lesser extent of protein destabilisation when compared to longer alkyl side chain groups: above [C2mim]+. Competition STD-NMR experiments using [C2mim]+, [C4mim]+ and [C2OHmim]+ also validate the importance of the hydrophobic interactions. The combined effect of cation and anion interactions was explored using 35Cl NMR. Such experiments show that the nature of the cation has no influence on the anion{\textendash}protein contacts, still the nature of the anion modulates the cation{\textendash}protein interaction. Herein we propose that more destabilising anions are likely to be a result of a partial contribution from the cation as a direct consequence of the different levels of interaction (cation{\textendash}anion pair and cation{\textendash}protein).

}, author = {Micael Silva and Angelo Miguel Figueiredo and Cabrita, Eurico J.} } @article {5698, title = {Inhibition of LOX by flavonoids: a structure-activity relationship study.}, journal = {European Journal of Medicinal Chemistry}, volume = {72}, year = {2014}, pages = {137-145}, abstract = {

The lipoxygenase (LOX) products have been identified as mediators of a series of inflammatory diseases, namely rheumatoid arthritis, inflammatory bowel disease, psoriasis, allergic rhinitis, atherosclerosis and certain types of cancer. Hence, LOX inhibitors are of interest for the modulation of these phenomena and resolution of the inflammatory processes. During LOX activity, peroxyl radical complexes are part of the reaction and may function as sources of free radicals. Thus antioxidants, such as flavonoids, capable of inhibiting lipid peroxidation and scavenging free radicals, may act as LOX inhibitors. The aim of this work was to assess the structure{\textendash}activity relationship among a series of flavonoids concerning 5-LOX inhibition, through a systematic study of the inhibition of the formation of LTB4 in human neutrophils. The type of inhibition of the flavonoids was further studied using soybean LOX, type I, and Saturation Transfer Difference 1H NMR (STD-1H NMR) was used to characterize the binding epitopes of the compounds to LOX-1. The obtained results reinforce flavonoids as effective inhibitors of LTB4 production in human neutrophils. It was also possible to establish a structure/activity relationship for the inhibitory activity and the type of inhibition.

}, url = {http://www.sciencedirect.com/science/article/pii/S0223523413007721}, author = {Ribeiro, D and Freitas, M and Tom{\'e}, SM and Silva, AM and Porto, G and Cabrita, E. J. and Marques, M. M. and Fernandes, E} } @article {3739, title = {Molecular Recognition of Rosmarinic Acid from Salvia sclareoides Extracts by Acetylcholinesterase: A New Binding Site Detected by NMR Spectroscopy}, journal = {Chemistry: A European Journal}, volume = {19}, year = {2013}, pages = {6641-6649}, abstract = {

Acetylcholinesterase (AChE) inhibition is one of the most currently available therapies for the management of Alzheimer{\textquoteright}s disease (AD) symptoms. In this context, NMR spectroscopy binding studies were accomplished to explain the inhibition of AChE activity by Salvia sclareoides extracts. HPLC-MS analyses of the acetone, butanol and water extracts eluted with methanol and acidified water showed that rosmarinic acid is present in all the studied samples and is a major constituent of butanol and water extracts. Moreover, luteolin 4'-O-glucoside, luteolin 3',7-di-O-glucoside and luteolin 7-O-(6''-O-acetylglucoside) were identified by MS2 and MS3 data acquired during the LC-MSn runs. Quantification of rosmarinic acid by HPLC with diode-array detection (DAD) showed that the butanol extract is the richest one in this component (134 μg mg-1 extract). Saturation transfer difference (STD) NMR spectroscopy binding experiments of S. sclareoides crude extracts in the presence of AChE in buffer solution determined rosmarinic acid as the only explicit binder for AChE. Furthermore, the binding epitope and the AChE-bound conformation of rosmarinic acid were further elucidated by STD and transferred NOE effect (trNOESY) experiments. As a control, NMR spectroscopy binding experiments were also carried out with pure rosmarinic acid, thus confirming the specific interaction and inhibition of this compound against AChE. The binding site of AChE for rosmarinic acid was also investigated by STD-based competition binding experiments using Donepezil, a drug currently used to treat AD, as a reference. These competition experiments demonstrated that rosmarinic acid does not compete with Donepezil for the same binding site. A 3D model of the molecular complex has been proposed. Therefore, the combination of the NMR spectroscopy based data with molecular modelling has permitted us to detect a new binding site in AChE, which could be used for future drug development.

}, url = {http://doi.org/10.1002/chem.201203966}, author = {Marcelo, Filipa and Dias, Catarina and Madeira, Paulo J. and Jorge, Tiago and Flor{\^e}ncio, M. Helena and Canada, F. Javier and Cabrita, Eurico J. and Jim{\'e}nez-Barbero, J{\'e}sus and Rauter, Amelia P.} } @article {4595, title = {NMR Solution Structure and SRP54M predicted interaction of the N-Terminal sequence (1-30) of the ovine Doppel protein}, journal = {Peptides}, volume = {49}, year = {2013}, pages = {32-40}, abstract = {

Prion protein (PrPC) biosynthesis involves a multi-step process that includes translation and post-translational modifications. While PrP has been widely investigated, for the homolog Doppel (Dpl), limited knowledge is available. In this study, we focused on a vital step of eukaryotic protein biosynthesis: targeting by the signal recognition particle (SRP). Taking the ovine Dpl (OvDpl(1-30)) peptide as a template, we studied its behavior in two different hydrophobic environments using CD and NMR spectroscopy. In both trifluoroethanol (TFE) and dihexanoyl-sn-glycero-3-phosphatidylcholine (DHPC), the OvDpl(1-30) peptide revealed to fold in an alpha-helical conformation with a well-defined central region extending from residue Cys8 until Ser22. The NMR structure was subsequently included in a computational docking complex with the conserved M-domain of SRP54 protein (SRP54M), and further compared with the N-terminal structures of mouse Dpl and bovine PrPC proteins. This allowed the determination of (i) common predicted N-terminal/SRP54M polar contacts (Asp331, Gln335, Glu365 and Lys432) and (ii) different N{\textendash}C orientations between prion and Dpl peptides at the SRP54M hydrophobic groove, that are in agreement with each peptide electrostatic potential. Together, these findings provide new insights into the biosynthesis of prion-like proteins. Besides they also show the role of protein conformational switches in signalization toward the endoplasmic membrane, a key event of major significance in the cell cycle. They are thus of general applicability to the study of the biological function of prion-like as well as other proteins.

}, url = {http://dx.doi.org/10.1016/j.peptides.2013.08.013}, author = {Jorge Pimenta and Viegas, Aldino and Sardinha, Jo{\~a}o and Martins, Ivo C and Cabrita, Eurico J. and Fontes, Carlos M. G. A. and Prates, Jose A. M. and Pereira, Rosa M. L. N.} } @article {4998, title = {Protein destabilisation in ionic liquids: the role of preferential interactions in denaturation}, journal = {Phys. Chem. Chem. Phys.}, year = {2013}, pages = {19632-19643}, abstract = {

The preferential binding of anions and cations in aqueous solutions of the ionic liquids (ILs) 1-butyl- 3-methylimidazolium ([C4mim]+) and 1-ethyl-3-methylimidazolium ([C2mim]+) chloride and dicyanamide (dca-) with the small alpha-helical protein Im7 was investigated using a combination of differential scanning calorimetry, NMR spectroscopy and molecular dynamics (MD) simulations. Our results show that direct ion interactions are crucial to understand the effects of ILs on the stability of proteins and that an anion effect is dominant. We show that the binding of weakly hydrated anions to positively charged or polar residues leads to the partial dehydration of the backbone groups, and is critical to control stability, explaining why dca- is more denaturing than Cl-. Direct cation{\textendash}protein interactions also mediate stability; cation size and hydrophobicity are relevant to account for destabilisation as shown by the effect of [C4mim]+ compared to [C2mim]+. The specificity in the interaction of IL ions with protein residues established by weak favourable interactions is confirmed by NMR chemical shift perturbation, amide hydrogen exchange data and MD simulations. Differences in specificity are due to the balance of interaction established between ion pairs and ion-solvent that determine the type of residues affected. When the interaction of both cation and anion with the protein is strong the net result is similar to a non-specific interaction, leading ultimately to unfolding. Since the nature of the ions is a determinant of the level of interaction with the protein towards denaturation or stabilisation, ILs offer a unique possibility to modulate protein stabilisation or even folding events.

}, url = {http://pubs.rsc.org/en/Content/ArticleLanding/2013/CP/c3cp53395f$\#$!divAbstract}, author = {Angelo Miguel Figueiredo and Sardinha, Jo{\~a}o and Moore, Geoffrey R. and Cabrita, Eurico J.} } @article {3233, title = {Solution Structure, Dynamics and Binding Studies of a Family 11 Carbohydrate-Binding Module from Clostridium thermocellum (CtCBM11)}, journal = {Biochemical Journal}, volume = {451}, year = {2013}, pages = {289-300}, abstract = {

Non-catalytic cellulosomal carbohydrate-binding modules (CBMs) are responsible for increasing the catalytic efficiency of cellulosic enzymes by selectively putting the substrate (a wide range of poly- and oligosaccharides) and enzyme into close contact. In the present work we carried out an atomistic rationalization of the molecular determinants of ligand specificity of a family 11 CBM from thermophilic C. thermocellum (CtCBM11), based on a NMR and molecular modeling approach. We have determined the NMR solution structure of CtCBM11 at 25 and 50 {\textordmasculine}C and derived information on the residues of the protein involved in ligand recognition and on the influence of the length of the saccharide chain on binding. We obtained models of the CtCBM11/cellohexaose and CtCBM11/cellotetraose complexes by docking in accordance with the NMR experimental data. Specific ligand/protein CH-π and Van der Waals interactions were found to be determinant for the stability of the complexes and for defining specificity. Using the order parameters derived from backbone dynamics analysis in the presence and absence of ligand and at 25 and 50 {\textordmasculine}C, we determined that the protein{\textquoteright}s backbone conformational entropy is slightly positive. This data in combination with the negative binding entropy calculated from ITC studies supports a selection mechanism where a rigid protein selects a defined oligosaccharide conformation.

}, url = {http://dx.doi.org/10.1042/BJ20120627}, author = {Viegas, Aldino and Sardinha, Jo{\~a}o and Duarte, Daniel F and Carvalho, Ana Luisa and Fontes, Carlos M. G. A. and Romao, Maria Joao and Macedo, Anjos L and Cabrita, Eurico J.} } @article {3238, title = {Synthesis and characterization of novel γ-induced porous PHEMA{\textendash}IL composites}, journal = {Materials Chemistry and Physics}, volume = {138}, year = {2013}, pages = {11-16}, abstract = {

A novel porous polymer-ionic liquid composite with poly(2-hydroxyethyl methacrylate) (PHEMA) and 1-butyl-3-methylimidazolium hexafluorophosphate (BMIPF6) has been synthesized by γ-irradiation without heat or chemical initiators. The products can be reversibly converted into organogels. The composites are potential candidates for electrochemical applications. The use of γ-radiation can be a simple and versatile alternative way to obtain these materials.

}, url = {http://dx.doi.org/10.1016/j.matchemphys.2012.11.061 }, author = {Casimiro, MH and Corvo, Marta C. and Ramos, A. M. and Cabrita, E. J. and Ramos, A. M. and Ferreira, LM} } @article {3113, title = { Thymus mastichina: Chemical Constituents and their Anti-cancer Activity }, journal = {Natural Product Communications}, volume = {7}, year = {2012}, pages = {1491-1494}, author = {Joana Gordo and Patr{\'\i}cia M{\'a}ximo and Eurico Cabrita and Ana Louren{\c c}o and Abel Oliva and Joana Almeida and Mariana Filipe and Pedro Cruz and Rita Barcia and Miguel Santos and Helder Cruz} } @article {2272, title = {Indole based cyclooxygenase inhibitors: synthesis, biological evaluation, docking and NMR screening}, journal = {European Journal of Medicinal Chemistry}, volume = {54}, year = {2012}, pages = {823-833}, abstract = {

The close structural similarity between the two cyclooxygenase (COXs) isoforms and the absence of selective inhibitors without side effects continues to stimulate the development of novel approaches towards selective anti-inflammatory drugs. In the present study a small library of new indolic compounds involving two different substitutions patterns at the indole scaffold was synthesized. In order to establish a relation between the spatial distribution of known functional groups related with inhibitory activity, two substitution patterns were explored: one with substituents at N-1, C-3, C-5 positions and another at C-2, C-3 and C5 positions. Accordingly, indole positions C-5, C-3 and N-1 were substituted with: sulfonamide or methylsulfone at C-5, p-halo-benzyl group at C-3, and an alkyl chain with a trifluoromethyl group at N-1. Alternatively, a p-halo-benzyl group was introduced at C-2, leaving the indolic nitrogen free. Inhibitory studies were performed and the activity results obtained against both COXs isoforms were rationalized based on docking and NMR studies. Docking studies show that dialkyation at C-2 and C-3 favors a binding with an orientation similar to that of the known selective inhibitor SC-558. From the tested compounds, this substitution pattern is correlated with the highest inhibitory activity and selectivity: 70\% COX-2 inhibition at 50 M, and low COX-1 inhibition (18{\textpm}9\%). Additionally, Saturation Transfer Difference NMR experiments reveal different interaction patterns with both COXs isoforms that may be related with different orientations of the sulfonamide group in the binding pocket. Despite the moderated inhibitory activities found, this study represents an innovative approach towards COXs inhibitory activity rationalization and to the design of anti-inflammatory drugs.

}, author = {M{\'o}nica S. Estev{\~a}o and Lu{\'\i}sa C. R. Carvalho and Marisa Freitas and Ana Gomes and Viegas, Aldino and Jo{\~a}o Manso and Stefan Erhadrt and Cabrita, Eurico J. and Marques, Maria Manuel B.} } @article {1427, title = {Platinated DNA Affects Zinc Finger Conformation. The Interaction of a Platinated Single-Stranded Oligonucleotide and the C-terminal Zinc Finger of the Nucleocapsid Protein HIVNCp7}, journal = {Biochemistry}, volume = {51}, year = {2012}, pages = {1752-1761}, abstract = {

This paper describes for the first time the intimate molecular details of the association between a platinated oligonucleotide and a zinc-finger peptide. Site-specific platination of the guanine in a ss hexanucleotide gave {[Pt(dien)d(5{\textquoteright}-TACGCC-3{\textquoteright})], Pt(dien)(6-mer)}, II, characterized by mass spectrometry and 1H-NMR spectroscopy. The work extends the study of platinum-nucleobase complex-zinc finger interactions using small molecules such as [Pt(dien)(9-EtGua)]2+, I . The structure of the (34-52) C-terminal finger of the HIV nucleocapsid protein HIVNCp7 (ZF1) was characterized by 1H-NMR spectroscopy and compared with that of the N-terminal single finger and the 2-finger {\textquotedblleft}intact{\textquotedblright} NCp7. Interaction of II with ZF1 results in significant changes in comparison to the {\textquotedblleft}free{\textquotedblright} uncomplexed hexanucleotide {\textendash} the major shifts occur for Trp37 resonances are broadened and shifted upfield and other major shifts are for Gln45 (H21, H3, Q), Met46 (NH, H2), Lys47 (NH, Q) and Glu50 (H2, H3). The Zn-Cys/His chemical shifts show only marginal deviations. The solution structure of ZF1, the 6-mer/ZF1 and II/ZF1 adducts were calculated from the NOESY-derived distance constraints. The DNA position in II/ZF1 is completely different than in the absence of platinum. Major differences are the appearance of new Met46-Cyt6H5 and Trp37-Cyt5H5 contacts but severe weakening of the Trp37-Gua4 contact, attributed to the steric effects caused by Gua4 platination, accompanied by a change in the position of the aromatic ring. The results demonstrate the feasibility of targetting specific ZF motifs with DNA-tethered coordination compounds, such as Pt compounds and Co-macrocycles {\textendash} with implications for drug targetting and indeed the intimate mechansims of DNA repair of platinated DNA.

}, url = {http://pubs.acs.org/doi/abs/10.1021/bi201834g}, author = {Susana Maria Quintal and Viegas, Aldino and Stefan Erhardt and Cabrita, Eurico J. and Nicholas P. Farrell} } @article {3115, title = {The Prion-like Protein Doppel Enhances Ovine Spermatozoa Fertilizing Ability}, journal = {Reproduction in Domestic Animals}, volume = {47}, year = {2012}, pages = {196-202}, abstract = {

The function of prion-like protein Doppel was suggested to be related to male fertility. In this study, the importance of ovine Doppel polypeptide on spermatozoa capacitation and fertilization was evaluated. After refolding, recombinant Doppel (rDpl) was supplemented with different concentrations (40, 80 or 190 ng/ml) to ovine spermatozoa during the capacitation process. In experiment 1, post-thawed ovine spermatozoa were incubated with different concentrations of rDpl during 1 h for swim-up, and changes in sperm motility, concentration, vigour, viability and capacitation were monitored (10 replicates). In experiment 2, the fertilization ability of post-swim-up spermatozoa incubated as above was tested through heterologous fertilization of bovine in vitro matured oocytes (n = 423, three replicates). Regardless of dosage, rDpl improved (p = 0.03) spermatozoa viability. Sperm individual motility and vigour were the highest (p = 0.04) for the group receiving 190 ng/ml rDpl. Sperm supplemented with the highest doses of rDpl achieved higher (p = 0.02) fertilization rates (56.0 +/- 3.0\%) than control (39.1 +/- 2.2\%) and 40 ng/ml rDpl (39.8 +/- 2.7\%). Preliminary data suggest that Doppel protein may enhance in vitro spermatozoa fertilizing ability.

}, author = {J. Pimenta and Dias, FMV and C. C. Marques and M. C. Baptista and M. I. Vasques and A. E. M. Horta and J. P. Barbas and R. Soares and P. Mesquita and E. Cabrita and Fontes, CMGA and J. A. Prates and R. M. Pereira} } @article {1002, title = {Synthesis of a new pyranoanthocyanin dimer linked through a methyl-methine bridge}, journal = {Tetrahedron Letters}, volume = {52}, number = {23}, year = {2011}, note = {n/a}, month = {JUN 8 2011}, pages = {2957-2960}, abstract = {n/a}, author = {Oliveira, Joana and Mateus, Nuno and Rodriguez-borges, Jose E. and Cabrita, Eurico J. and Silva, Artur M. S. and de Freitas, Victor} } @article {1007, title = {Quantification of organic acids in beer by nuclear magnetic resonance (NMR)-based methods}, journal = {Analytica Chimica Acta}, volume = {674}, number = {2}, year = {2010}, note = {n/a}, month = {AUG 3 2010}, pages = {166-175}, abstract = {n/a}, author = {Rodrigues, J. E. A. and Erny, G. L. and Barros, A. S. and Esteves, V. I. and Brandao, T. and Ferreira, A. A. and E. Cabrita and Gil, A. M.} } @article {1008, title = {Asymmetric Intramolecular Aldol Reactions of Substituted 1,7-Dicarbonylic Compounds. A Mechanistic Study}, journal = {Journal of Organic Chemistry}, volume = {75}, number = {8}, year = {2010}, note = {n/a}, month = {APR 16 2010}, pages = {2546-2555}, abstract = {n/a}, author = {Duarte, F. J. S. and Cabrita, E. J. and Frenking, G. and A. Gil Santos} } @article {1014, title = {Density Functional Study of Proline-Catalyzed Intramolecular Baylis-Hillman Reactions}, journal = {Chemistry-a European Journal}, volume = {15}, number = {7}, year = {2009}, note = {n/a}, month = {2009}, pages = {1734-1746}, abstract = {n/a}, author = {Duarte, Filipe J. S. and Cabrita, Eurico J. and Frenking, Gernot and A. Gil Santos} } @article {1016, title = {Molecular determinants of ligand specificity in family 11 carbohydrate binding modules - an NMR, X-ray crystallography and computational chemistry approach}, journal = {Febs Journal}, volume = {275}, number = {10}, year = {2008}, note = {n/a}, month = {MAY 2008}, pages = {2524-2535}, abstract = {n/a}, author = {Viegas, Aldino and Bras, Natercia F. and Cerqueira, Nuno M. F. S. A. and Fernandes, Pedro Alexandrino and Prates, Jose A. M. and Fontes, Carlos M. G. A. and Bruix, Marta and Romao, Maria Joao and Carvalho, Ana Luisa and Ramos, Maria Joao and Macedo, Anjos L and Cabrita, Eurico J.} } @article {1018, title = {Mechanistic study of intramolecular aldol reactions of dialdehydes}, journal = {European Journal of Organic Chemistry}, number = {19}, year = {2008}, note = {n/a}, month = {JUL 2008}, pages = {3397-3402}, abstract = {n/a}, author = {Duarte, Filipe J. S. and Cabrita, Eurico J. and Frenking, Gernot and Santos, Gil} }