Publications

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2015
Synthesis and evaluation of new benzimidazole-based COX inhibitors: a naproxen-like interaction detected by STD-NMR, Carvalho, L. C. R., Ribeiro D., Seixas R. S. G. R., Silva A. M. S., Nave M., Martins A. C., Erhardt S., Fernandes E., Cabrita E. J., and Marques M. M. B. , RSC Advances, Volume 5, p.49098-49109, (2015) AbstractWebsite

Non-steroidal anti-inflammatory drugs exert their pharmacological activity through inhibition of cyclooxygenase 1 and 2 (COX-1 and COX-2). Recent research suggests that a balanced inhibition of both COX-1 and COX-2 is the key to reduce the side-effects exhibited by COX inhibitors. We developed new benzimidazole-based compounds that showed a balanced COX inhibition, supported by molecular docking screening. The human whole blood assays demonstrated that the ester derivatives were potent inhibitors. Competitive saturation transfer difference (STD)-NMR experiments, in the presence of COX-2, using naproxen and diclofenac demonstrated that ester derivatives do not compete with diclofenac for the same binding site, but compete with the allosteric inhibitor naproxen. Combination of NMR spectroscopy with molecular docking has permitted us to detect a new naproxen-like inhibitor, which could be used for future drug development.

2013
Shaping the molecular assemblies of native and alkali- modified agars in dilute and concentrated aqueous media via microwave-assisted extraction, Sousa, Ana M. M., Borges João, Silva Fernando, Ramos Ana M., Cabrita Eurico J., and Gonçalves Maria Pilar , Soft Matter, Volume 9, p.3131-3139, (2013) AbstractWebsite

The use of agar-based biomaterials for the development of emerging areas, such as tissue engineering or ‘smart materials’ production has recently gained great interest. Understanding how these gel-forming polysaccharides self-organise in aqueous media and how these associations can be tuned to meet the specific needs of each application is thus of great relevance. As an extension of previous pioneering research concerning the application of the microwave-assisted extraction (MAE) technique in the recovery of native (NA) and alkali-modified (AA) agars, this article focuses on the different molecular assemblies assumed by these novel NA and AA when using different MAE routes. The molecular architectures in dilute (5, 10, 50 and 100 mg mL1) and concentrated (1.5% (w/w)) aqueous media were imaged by AFM and cryoSEM, respectively. Relevant structural and physicochemical properties were investigated to support the microscopic data. Different extraction routes led to polysaccharides with unique properties, which in turn resulted in different molecular assemblies. Even at 5 mg mL1, AFM images included individual fibers, cyclic segments, aggregates and local networks. At higher polymer concentrations, the structures further aggregated forming multilayer polymeric networks for AA. The more compact and denser 3D networks of AA, imaged by cryoSEM, and their higher resistance to large deformations matched the 2D-shapes observed by AFM. Depending on the nature of the AA chains, homogeneous or heterogeneous growth of assemblies was seen during network formation. The obtained results support well the view of double helix formation followed by intensive double helix association proposed for agar gelation.

Solution Structure, Dynamics and Binding Studies of a Family 11 Carbohydrate-Binding Module from Clostridium thermocellum (CtCBM11), Viegas, Aldino, Sardinha João, Duarte Daniel F., Carvalho Ana Luisa, Fontes Carlos M. G. A., Romao Maria Joao, Macedo Anjos L., and Cabrita Eurico J. , Biochemical Journal, Volume 451, p.289-300, (2013) AbstractWebsite

Non-catalytic cellulosomal carbohydrate-binding modules (CBMs) are responsible for increasing the catalytic efficiency of cellulosic enzymes by selectively putting the substrate (a wide range of poly- and oligosaccharides) and enzyme into close contact. In the present work we carried out an atomistic rationalization of the molecular determinants of ligand specificity of a family 11 CBM from thermophilic C. thermocellum (CtCBM11), based on a NMR and molecular modeling approach. We have determined the NMR solution structure of CtCBM11 at 25 and 50 ºC and derived information on the residues of the protein involved in ligand recognition and on the influence of the length of the saccharide chain on binding. We obtained models of the CtCBM11/cellohexaose and CtCBM11/cellotetraose complexes by docking in accordance with the NMR experimental data. Specific ligand/protein CH-π and Van der Waals interactions were found to be determinant for the stability of the complexes and for defining specificity. Using the order parameters derived from backbone dynamics analysis in the presence and absence of ligand and at 25 and 50 ºC, we determined that the protein’s backbone conformational entropy is slightly positive. This data in combination with the negative binding entropy calculated from ITC studies supports a selection mechanism where a rigid protein selects a defined oligosaccharide conformation.

Solvation of CO2 in [C4mim][BF4] and [C4mim][PF6] ionic liquids revealed by High Pressure NMR, Corvo, Marta C., Sardinha João, Menezes Sonia C., Einloft Sandra, Seferin Marcus, Dupont Jairton, Casimiro Teresa, and Cabrita Eurico J. , Angewandte Chemie International Edition, Volume 49, Issue 52, p.13024-13027, (2013) Website
Synthesis and characterization of novel γ-induced porous PHEMA–IL composites, Casimiro, MH, Corvo Marta C., Ramos A. M., Cabrita E. J., Ramos A. M., and Ferreira LM , Materials Chemistry and Physics, Volume 138, Issue 1, p.11-16, (2013) AbstractWebsite

A novel porous polymer-ionic liquid composite with poly(2-hydroxyethyl methacrylate) (PHEMA) and 1-butyl-3-methylimidazolium hexafluorophosphate (BMIPF6) has been synthesized by γ-irradiation without heat or chemical initiators. The products can be reversibly converted into organogels. The composites are potential candidates for electrochemical applications. The use of γ-radiation can be a simple and versatile alternative way to obtain these materials.

2012
Structural, Physical, and Chemical Modifications Induced by Microwave Heating on Native Agar-like Galactans, Sousa, Ana M. M., Morais Simone, Abreu Maria H., Pereira Rui, Sousa-Pinto Isabel, Cabrita Eurico J., Delerue-Matos Cristina, and Gonca̧lves Maria Pilar , Jornal of Agricultural and Food Chemistry , Volume 60, p.4977-4985, (2012) Abstract

Native agars from Gracilaria vermiculophylla produced in sustainable aquaculture systems (IMTA) were extracted under conventional (TWE) and microwave (MAE) heating. The optimal extracts from both processes were compared in terms of their properties. The agars’ structure was further investigated through Fourier transform infrared and NMR spectroscopy. Both samples showed a regular structure with an identical backbone, β-D-galactose (G) and 3,6-anhydro-α-L-galactose (LA) units; a considerable degree of methylation was found at C6 of the G units and, to a lesser extent, at C2 of the LA residues. The methylation degree in the G units was lower for MAEopt agar; the sulfate content was also reduced. MAE led to higher agar recoveries with drastic extraction time and solvent volume reductions. Two times lower values of [η] and Mv obtained for the MAEopt sample indicate substantial depolymerization of the polysaccharide backbone; this was reflected in its gelling properties; yet it was clearly appropriate for commercial application in soft-texture food products.

2011
Synthesis of a new pyranoanthocyanin dimer linked through a methyl-methine bridge, Oliveira, Joana, Mateus Nuno, Rodriguez-borges Jose E., Cabrita Eurico J., Silva Artur M. S., and de Freitas Victor , Tetrahedron Letters, JUN 8 2011, Volume 52, Number 23, p.2957-2960, (2011) Abstract
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Saturation-Transfer Difference (STD) NMR: A Simple and Fast Method for Ligand Screening and Characterization of Protein Binding, Viegas, Aldino, Manso Joao, Nobrega Franklin L., and Cabrita Eurico J. , Journal of Chemical Education, JUL 2011, Volume 88, Number 7, p.990-994, (2011) AbstractWebsite

Saturation transfer difference (STD) NMR has emerged as one of the most popular ligand-based NMR techniques for the study of protein−ligand interactions. The success of this technique is a consequence of its robustness and the fact that it is focused on the signals of the ligand, without any need of processing NMR information about the receptor and only using small quantities of nonlabeled macromolecule. Moreover, the attractiveness of this experiment is also extendable to the classroom. In the context of a practical NMR class, this experiment is ideal to illustrate some fundamental NMR concepts, such as the nuclear Overhauser effect and relaxation in a multidisciplinary context, bridging chemistry and biochemistry with a taste of medicinal chemistry.

We use the readily available human serum albumin (HSA), 6-d,l-methyl-tryptophan (6-CH3-Trp), and 7- d,l-methyl-tryptophan (7-CH3-Trp) to introduce the STD-NMR experiment and to illustrate its applicability for ligand screening, mapping of binding moieties, and determination of the dissociation constant, in a context that can be explored or adapted to the student’s course level and topic (chemistry or biochemistry). We also cover the most important theoretical aspects of the STD experiment, calling attention to some of its limitations and drawbacks without a complex theoretical approach.

Screening nucleotide binding to amino acid-coated supports by surface plasmon resonance and nuclear magnetic resonance, Cruz, Carla, Cabrita Eurico J., and Queiroz Joao A. , Analytical and Bioanalytical Chemistry, AUG 2011, Volume 401, Number 3, p.983-993, (2011) Abstract
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2003
Studies on the preparation of 4-ethoxyalkyliden and 4-aminoalkyliden-5(4H)-oxazolones, Matos, MRPN, Gois PMP, Mata MLEN, Cabrita E. J., and Afonso CAM , Synthetic Communications, 2003, Volume 33, Number 8, p.1285-1299, (2003) Abstract
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