Publications

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2015
The Quest for Anticancer Vaccines: Deciphering the Fine-Epitope Specificity of Cancer-Related Monoclonal Antibodies by Combining Microarray Screening and Saturation Transfer Difference NMR, Coelho, Helena, Matsushita T., Artigas G., Hinou H., Cañada FJ, Lo-Man R., Leclerc C., Cabrita E. J., Jiménez-Barbero J., Nishimura S. - I., Garcia-Martín F., and Marcelo F. , J. Am. Chem. Soc., Volume 137, p.12438-12441, (2015)
2012
The Prion-like Protein Doppel Enhances Ovine Spermatozoa Fertilizing Ability, Pimenta, J., Dias FMV, Marques C. C., Baptista M. C., Vasques M. I., Horta A. E. M., Barbas J. P., Soares R., Mesquita P., Cabrita E., Fontes CMGA, Prates J. A., and Pereira R. M. , Reproduction in Domestic Animals, Volume 47, Issue 2, p.196-202, (2012) Abstract

The function of prion-like protein Doppel was suggested to be related to male fertility. In this study, the importance of ovine Doppel polypeptide on spermatozoa capacitation and fertilization was evaluated. After refolding, recombinant Doppel (rDpl) was supplemented with different concentrations (40, 80 or 190 ng/ml) to ovine spermatozoa during the capacitation process. In experiment 1, post-thawed ovine spermatozoa were incubated with different concentrations of rDpl during 1 h for swim-up, and changes in sperm motility, concentration, vigour, viability and capacitation were monitored (10 replicates). In experiment 2, the fertilization ability of post-swim-up spermatozoa incubated as above was tested through heterologous fertilization of bovine in vitro matured oocytes (n = 423, three replicates). Regardless of dosage, rDpl improved (p = 0.03) spermatozoa viability. Sperm individual motility and vigour were the highest (p = 0.04) for the group receiving 190 ng/ml rDpl. Sperm supplemented with the highest doses of rDpl achieved higher (p = 0.02) fertilization rates (56.0 +/- 3.0%) than control (39.1 +/- 2.2%) and 40 ng/ml rDpl (39.8 +/- 2.7%). Preliminary data suggest that Doppel protein may enhance in vitro spermatozoa fertilizing ability.

2007
Residue-specific NH exchange rates studied by NMR diffusion experiments (vol 187, pg 97, 2007), Brand, Torsten, Cabrita Eurico J., Morris Gareth A., Guenther Robert, Hofmann Hans-Joerg, and Berger Stefan , Journal of Magnetic Resonance, OCT 2007, Volume 188, Number 2, p.387-387, (2007) Abstract
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Residue-specific NH exchange rates studied by NMR diffusion experiments, Brand, Torsten, Cabrita Eurico J., Morris Gareth A., Guenther Robert, Hofmann Hans-Jorg, and Berger Stefan , Journal of Magnetic Resonance, JUL 2007, Volume 187, Number 1, p.97-104, (2007) Abstract
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1998
Flexible molecules with defined shape. X. Synthesis and conformational study of 1,5-diaza-cis-decalin, Santos, AGD, Klute W., Torode J., Bohm V. P. W., Cabrita E., Runsink J., and Hoffmann RW , New Journal of Chemistry, SEP 1998, Volume 22, Number 9, p.993-997, (1998) Abstract
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