@article {Costa2007,
	title = {{Supercritical antisolvent precipitation of PHBV microparticles}},
	journal = {International Journal of Pharmaceutics},
	volume = {328},
	number = {1 SPEC. ISS.},
	year = {2007},
	note = {n/a},
	pages = {72{\textendash}77},
	abstract = {<p>The micronization of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) from organic solutions using supercritical antisolvent (SAS) technique has been successfully achieved. SAS experiments were carried out at different operational conditions and microspheres with mean diameters ranging from 3 to 9 $μ$m were obtained. The effect of CO2 and liquid flow, temperature and pressure on particle size and particle size distribution was evaluated. The microspheres were precipitated from a dichloromethane (DCM) solution. The best process conditions for this mixture were, according to our study, 40 {\textdegree}C, 100 bar, 1 mL min-1 liquid flow and 10 L min-1 carbon dioxide flow. Experiments with polymers containing different HV percentages were carried out. The powders obtained became more spherical as the HV content decreased. {\textcopyright} 2006 Elsevier B.V. All rights reserved.</p>
},
	keywords = {Drug delivery, Micronization, natural polymers, PHBV, SAS, Supercritical fluids},
	isbn = {0032-5910},
	issn = {03785173},
	doi = {10.1016/j.ijpharm.2006.08.004},
	author = {Costa, Mariana Sousa and Duarte, Ana Rita C and Cardoso, M. Margarida and Duarte, Catarina M.M.}
}
