@article {Silva2016,
	title = {{Extraction of Collagen/Gelatin from the Marine Demosponge Chondrosia reniformis (Nardo, 1847) Using Water Acidified with Carbon Dioxide - Process Optimization}},
	journal = {Industrial and Engineering Chemistry Research},
	volume = {55},
	number = {25},
	year = {2016},
	note = {n/a},
	pages = {6922{\textendash}6930},
	abstract = {<p>Marine sponges are a rich source of natural bioactive compounds. One of the most abundant valuable products is collagen/gelatin, which presents an interesting alternative source for pharmaceutical and biomedical applications. We have previously proposed an innovative green technology for the extraction of collagen/gelatin from marine sponges based in water acidified with carbon dioxide. In this work, we have optimized the process operating conditions toward high yields and collagen quality as well as to reduce extraction procedure duration and energy consumption. The process extraction efficiency is higher than 50{\%}, corresponding to a yield of approximately 10{\%} of the sponge dry mass, obtained for mild operating conditions of 10 bar and 3 h. The extracted material was characterized by scanning electron microscopy (SEM), rheology, Fourier transformed infrared spectroscopy (FTIR), circular dichroism (CD), amino acid analysis, and SDS-PAGE. The extracts were found to be composed of highly pure mixtures of co...</p>
},
	issn = {15205045},
	doi = {10.1021/acs.iecr.6b00523},
	author = {Silva, Jo{\~a}o C. and Barros, Alexandre A. and Aroso, Ivo M and Fassini, Dario and Silva, Tiago H. and Reis, Rui L and Duarte, Ana Rita C}
}
