<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="6.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Palma, A. S.</style></author><author><style face="normal" font="default" size="100%">Liu, Y.</style></author><author><style face="normal" font="default" size="100%">Zhang, H.</style></author><author><style face="normal" font="default" size="100%">Zhang, Y</style></author><author><style face="normal" font="default" size="100%">McCleary, B. V.</style></author><author><style face="normal" font="default" size="100%">Yu, G.</style></author><author><style face="normal" font="default" size="100%">Huang, Q.</style></author><author><style face="normal" font="default" size="100%">Guidolin, L. S.</style></author><author><style face="normal" font="default" size="100%">Ciocchini, A. E.</style></author><author><style face="normal" font="default" size="100%">Torosantucci, A.</style></author><author><style face="normal" font="default" size="100%">Wang, D.</style></author><author><style face="normal" font="default" size="100%">Carvalho, AL</style></author><author><style face="normal" font="default" size="100%">Fontes, C. M.</style></author><author><style face="normal" font="default" size="100%">Mulloy, B.</style></author><author><style face="normal" font="default" size="100%">Childs, R. A.</style></author><author><style face="normal" font="default" size="100%">Feizi, T.</style></author><author><style face="normal" font="default" size="100%">Chai, W.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Unravelling glucan recognition systems by glycome microarrays using the designer approach and mass spectrometry</style></title><secondary-title><style face="normal" font="default" size="100%">Mol Cell Proteomics</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2015</style></year></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/25670804</style></url></web-urls></urls><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Glucans are polymers of D-glucose with differing linkages in linear or branched sequences. They are constituents of microbial and plant cell-walls and involved in important bio-recognition processes including immunomodulation, anti-cancer activities, pathogen virulence and plant cell-wall biodegradation. Translational possibilities for these activities in medicine and biotechnology are considerable. High-throughput micro-methods are needed to screen proteins for recognition of specific glucan sequences as a lead to structure-function studies and their exploitation. We describe construction of a glucome microarray, the first sequence-defined glycome-scale microarray, using a designer approach from targeted ligand-bearing glucans in conjunction with a novel high-sensitivity mass spectrometric sequencing method, as a screening tool to assign glucan recognition motifs. The glucome microarray comprises 153 oligosaccharide probes with high purity, representing major sequences in glucans. The negative-ion electrospray tandem mass spectrometry with collision-induced dissociation was used for complete linkage analysis of gluco-oligosaccharides in linear homo and hetero and branched sequences. The system is validated using antibodies and carbohydrate-binding modules known to target α- or β-glucans in different biological contexts, extending knowledge on their specificities, and applied to reveal new information on glucan recognition by two signalling molecules of the immune system against pathogens: Dectin-1 and DC-SIGN. The sequencing of the glucan oligosaccharides by the MS method and their interrogation on the microarrays provides detailed information on linkage, sequence and chain length requirements of glucan-recognizing proteins, and are a sensitive means of revealing unsuspected sequences in the polysaccharides.&lt;/p&gt;
</style></abstract><work-type><style face="normal" font="default" size="100%">Journal Article</style></work-type><notes><style face="normal" font="default" size="100%">&lt;p&gt;n/a&lt;/p&gt;
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