<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="6.x">Drupal-Biblio</source-app><ref-type>5</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Bras, Joana L. A.</style></author><author><style face="normal" font="default" size="100%">Carvalho, Ana Luisa</style></author><author><style face="normal" font="default" size="100%">Viegas, Aldino</style></author><author><style face="normal" font="default" size="100%">Najmudin, Shabir</style></author><author><style face="normal" font="default" size="100%">Alves, Victor D.</style></author><author><style face="normal" font="default" size="100%">Prates, Jose A. M.</style></author><author><style face="normal" font="default" size="100%">Ferreira, Luis M. A.</style></author><author><style face="normal" font="default" size="100%">Romao, Maria J.</style></author><author><style face="normal" font="default" size="100%">Gilbert, Harry J.</style></author><author><style face="normal" font="default" size="100%">Fontes, Carlos M. G. A.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Escherichia coli expression, purification, crystallization, and structure determination of bacterial cohesin-dockerin complexes</style></title><secondary-title><style face="normal" font="default" size="100%">Methods in enzymology</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2012</style></year></dates><urls><web-urls><url><style face="normal" font="default" size="100%">&lt;Go to ISI&gt;://MEDLINE:22608738</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">510</style></volume><pages><style face="normal" font="default" size="100%">395-415</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Cellulosomes are highly efficient nanomachines that play a fundamental role during the anaerobic deconstruction of complex plant cell wall carbohydrates. The assembly of these complex nanomachines results from the very tight binding of repetitive cohesin modules, located in a noncatalytic molecular scaffold, and dockerin domains located at the C-terminus of the enzyme components of the cellulosome. The number of enzymes found in a cellulosome varies but may reach more than 100 catalytic subunits if cellulosomes are further organized in polycellulosomes, through a second type of cohesin-dockerin interaction. Structural studies have revealed how the cohesin-dockerin interaction mediates cellulosome assembly and cell-surface attachment, while retaining the flexibility required to potentiate catalytic synergy within the complex. Methods that might be applied for the production, purification, and structure determination of cohesin-dockerin complexes are described here. Copyright 2012 Elsevier Inc. All rights reserved.&lt;/p&gt;
</style></abstract><work-type><style face="normal" font="default" size="100%">Journal Article</style></work-type><notes><style face="normal" font="default" size="100%">&lt;p&gt;22608738&lt;/p&gt;
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